We narrowed to 931 results for: TIM;

...modifying these tools for COVID-19 detection with the ultimate goal to develop low cost and quick point-of-care...SARS-CoV-2 ORFs and include plasmids that are codon optimized. Browse the collection or learn more about it ...

...companies and start-ups choose to locate there. I made time to go into the city of Guangzhou and see some of...surprising” small RNAs can be and posited that “we underestimate living things.” He further cautioned the distinction...

...modifications in real-time, posing a huge challenge to studying epigenetic changes over time.  This challenge...

...the mutants, analyse their motor behaviour, and ultimately submit our new strains back to the CGC for other...differs from that of wild cats with the goal of optimizing the health of domesticated cats and learning ...

...we have analyzed hundreds of viral vectors and estimate that recombinase-independent recombination occurs...that users titrate their AAV vectors to find the optimal dose that allows for sufficient transgene expression...

...eclosion at the start of the day and throughout daytime. They mostly stop emerging at night. An incubator...recombine chromosomes during meiosis. This can sometimes be a handy feature when setting up genetic crosses...

...interest. Scientists are continuously updating and optimizing FP tools to avoid these problems. See, for example...example, the recently developed FPs optimized for diverse cellular environments. Since colocalization ...

...without the need to find a llama farm! To find an optimally binding sybody, you want to start out with a large...protocol goes through 3 major steps to select for optimal sybody candidates. Figure 2: Outline of the...

...be distinguished using conventional flow can (sometimes) be distinguished using spectral flow since the...signal, even for rare cellular markers, beads can sometimes distort the fluorescent signature upon antibody...

...recombination, but with longer homology arms comes more time spent cloning. In contrast, the short homology required...the risk of off-target effects, Sakuma et al. optimized the PITCh-gRNA for minimal off-target binding ...

...acquire one image, thereby significantly reducing the time it takes to image through a large z-stack (8).  ...compatible with LSFM, a method termed COLM (clarity-optimized light-sheet microscopy) (16).  Both 3DISCO and...

...restriction enzyme cloning, were more prohibitive at the time. With these limitations no longer an issue, bacterial...your life Rather than making up your PCR mix every time, pre-made aliquots of PCR master-mix, containing...

...cloning site (MCS). Modules are assembled one at a time by standard restriction enzyme digestion followed...DNA sequences, no restriction enzymes needed Not optimal for joining sequences with a high degree of identity...

...like FRET, BiFC experiments must be carefully optimized to be successful. A BiFC experiment generally ...inflammation and neurodegeneration. He now spends his time learning and writing about science whenever he's...

...can try re-purifying the sample you have, but if time and resources allow, you might be better off starting...flow-through solutions and use clean tubes? How about spin times, were these long enough?  There are, of course, ...

...disrupted. As our ESCs die and differentiate over time, we periodically use next-generation sequencing ...CRISPR. Therefore if a gRNA drops or disappears over time, we infer that the enhancer it targets is a “pillar...

... of bait and prey against each other at the same time in order to try and access the entire interactome...channel interactions detected by a genetic system optimized for systematic studies of membrane protein interactions...

...regulated to ensure that it occurs at the appropriate time—for example, the cell must have enough nutrients... its job — although ATP does get hydrolyzed over time, inactivating the protein binding it (Grimwade et...

...initial assumption. Eugene Koonin and Kira Makarova, longtime collaborators of Omar and Jonathan and experts...integration activity in human cells. Moreover, they optimized the specific attachment sites via sequence engineering...

... at low concentrations and/or are too expensive, time-consuming, and complex to use outside of standard...RNase activity of the Cas13 protein to develop and optimize the method termed Specific High Sensitivity Enzymatic...