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Showing: 661 - 680 of 925 results

  1. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type
    Blog Post
    ...companies and start-ups choose to locate there. I made time to go into the city of Guangzhou and see some of...surprising” small RNAs can be and posited that “we underestimate living things.” He further cautioned the distinction...

  3. Engaging with science and society at pgEd

    Type
    Blog Post
    ...for a year, I’ve since returned to pgEd as a full-time staff member for the past three years. pgEd is a...courses in social, health or policy-related topics, as time permits. Also seek out opportunities to communicate...

  4. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    ...the mutants, analyse their motor behaviour, and ultimately submit our new strains back to the CGC for other...differs from that of wild cats with the goal of optimizing the health of domesticated cats and learning ...

  5. Antibodies 101: Conventional vs Spectral Flow Cytometry

    Type
    Blog Post
    ...be distinguished using conventional flow can (sometimes) be distinguished using spectral flow since the...signal, even for rare cellular markers, beads can sometimes distort the fluorescent signature upon antibody...

  6. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type
    Blog Post
    ...we have analyzed hundreds of viral vectors and estimate that recombinase-independent recombination occurs...that users titrate their AAV vectors to find the optimal dose that allows for sufficient transgene expression...

  9. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    ...recombination, but with longer homology arms comes more time spent cloning. In contrast, the short homology required...the risk of off-target effects, Sakuma et al. optimized the PITCh-gRNA for minimal off-target binding ...

  11. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    ...acquire one image, thereby significantly reducing the time it takes to image through a large z-stack (8).  ...compatible with LSFM, a method termed COLM (clarity-optimized light-sheet microscopy) (16).  Both 3DISCO and...

  12. MXS Chaining

    Type
    Blog Post
    ...cloning site (MCS). Modules are assembled one at a time by standard restriction enzyme digestion followed...DNA sequences, no restriction enzymes needed Not optimal for joining sequences with a high degree of identity...

  15. Tips for Screening with Yeast Two Hybrid Systems

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    Blog Post
    ... of bait and prey against each other at the same time in order to try and access the entire interactome...channel interactions detected by a genetic system optimized for systematic studies of membrane protein interactions...

  16. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    ...restriction enzyme cloning, were more prohibitive at the time. With these limitations no longer an issue, bacterial...your life Rather than making up your PCR mix every time, pre-made aliquots of PCR master-mix, containing...

  17. Plasmids 101: Stringent Regulation of Replication

    Type
    Blog Post
    ...regulated to ensure that it occurs at the appropriate time—for example, the cell must have enough nutrients... its job — although ATP does get hydrolyzed over time, inactivating the protein binding it (Grimwade et...

  18. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    ...notebooks in the ELN, without having to add at the time of deposit, makes it far more likely that deposits...commercialization community in greater Boston, while at the same time keeping one foot on the other side of the pond through...

  19. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    ... at low concentrations and/or are too expensive, time-consuming, and complex to use outside of standard...RNase activity of the Cas13 protein to develop and optimize the method termed Specific High Sensitivity Enzymatic...

  20. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    ...initial assumption. Eugene Koonin and Kira Makarova, longtime collaborators of Omar and Jonathan and experts...integration activity in human cells. Moreover, they optimized the specific attachment sites via sequence engineering...
Showing: 661 - 680 of 925 results