We narrowed to 747 results for: UTY
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TypeBlog Post...vector Promoter driving Cas9 expression can be constitutive or inducible. U6 promoter is typically used ...
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CRISPR 101: Making a Knock-In Cell Line
TypeBlog Post...easiest way to make a knock-in cell line is to utilize the built-in repair pathway cells already have ... -
Uncovering Ancestral Bias in CRISPR Research
TypeBlog Post...deprioritize genes that could be good targets for therapeutic development, particularly in cancers that are... -
Capturing a Moment with Marc Zimmer’s “The State of Science”
TypeBlog Post...that “Addgene, with its easy, fast, and cheap distribution of the newest CRISPR-Cas plasmids, has been ... -
A Guide to Starting Your Own Journal Club
TypeBlog Post...failing in many academic journal clubs where institutional inertia drives the meeting rather than interest... -
Simplify Cloning with in vivo Assembly
TypeBlog Post...template DNA to minimize false positive colonies. We routinely screen just two colonies for simple cloning procedures... -
Negative Can Be Positive: Open AAV Data with Addgene
TypeBlog Post...information out into the public where it can be utilized by others. Addgene’s AAV Data Hub is one resource... -
Better Dyeing Through Chemistry & Small Molecule Fluorophores
TypeBlog Post... ligand exhibits low fluorescence when free in solution, but high fluorescence upon binding to its cognate... -
CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics
TypeBlog Post... the foundation of modern drug discovery and therapeutics. We’ll have more to say about clinical applications... -
CRISPR Antimicrobials
TypeBlog Post...Beisel et al., licensed under a Creative Commons Attribution 4.0 International License. Improving CRISPR... -
Cpf1: A New Tool for CRISPR Genome Editing
TypeBlog Post...Addgene. Makarova, Kira S., et al. "An updated evolutionary classification of CRISPR-Cas systems." Nat Rev... -
A Career in Grants: How To Become a Grant Professional
TypeBlog Post...federal regulations. A grant professional’s job duties can range from coordinating the grant application... -
Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More
TypeBlog Post...specific DNA sequences. Thus, we've made some contributions in technology development in biology and hope...involvement in the scientific community is a kind of duty for researchers when considering the peer-review... -
Fluorescent Proteins 101: Green Fluorescent Protein (GFP)
TypeBlog Post...mainstay in molecular biology. Scientists can easily utilize GFP-containing plasmids as a means to many functional... -
Your Lentiviral Plasmid FAQs Answered
TypeBlog Post... for using such reagents as outlined by your institution or country. Have any other questions? Feel free... -
PITChing MMEJ as an Alternative Route for Gene Editing
TypeBlog Post...About Other Genome Engineering Tools like Sleeping Beauty Mutase Find the Ideal CRISPR Software for Your ... -
Transfection for Recombinant Antibodies
TypeProtocol...ensure that you are in compliance with your institution’s biosafety regulations. Last Update: February...Leupeptin, Millipore Sigma L2884 Aprotinin saline solution, Millipore Sigma A6279 Before Starting Warm the...µm filter. Prepare 10 mL aliquots and store the solution at -20 °C. 1 mg/mL PEI-MAX Add 1 g of PEI-MAX ...but check for the presence of particles still in solution. Continue to stir until all particles have dissolved... to ensure that it has not drifted. Filter the solution through a 0.22 µm filter. Prepare aliquots and...at 4 °C until use. We suggest preparing fresh solutions after one month. BCD Feed 500 mL BalanCD HEK293...at 4 °C until use. We suggest preparing fresh solutions after one month. 1000X protease inhibitor cocktail... -
Harnessing TnpB for Genome Editing: A Compact and Versatile Tool for Your Research
TypeBlog Post...TAMs, and ωRNA (reRNA) TnpBs are thought to be evolutionary ancestors of Cas12 proteins. Functionally, while... -
Hot Plasmids: Fall 2024
TypeBlog Post...PILATeS suitable for live imaging with a time resolution of minutes. Find PILATeS plasmids here! Zhong... -
Twenty Years of Sharing: Addgene's Viral Vector Service
TypeBlog Post... AAVs preps from 2016–2023. B) Inset showing distribution of only GCaMP6, jGCaMP7, and jGCaMP8 per year...