We narrowed to 771 results for: VARS

1. The time and cost required to make a plasmid

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   ...designing and performing the experiments. This cost varies hugely based on a number of factors, but we can...

2. Plasmids 101: The protein expression toolbox

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   ...a minimal promoter. These systems come in two varieties: tet-on and tet-off. As the names imply, tet-off...

3. All in a Twist: dsRNA

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   ...exists within the R-loop that is now vulnerable to various forms of DNA damage, and these structures can in...

4. Supporting Rare Disease Research with the 2020 BeHEARD Awards

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   .... So far, there have been thirty GNB1 missense variants identified from 58 individuals with GNB1 disorder...

5. CRISPRainbow and Genome Visualization

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   ... other orthologs. Ma et al. tested their dCas9 variants using gRNAs specific for telomeric sequences and...

6. Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

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   ...for Science Symposium and Workshop Learn about a Variety of Science Careers Resources on Addgene.org Find...

7. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

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   ...Cloning Technique page and the Addgene Blog for a variety of useful resources and protocols! We invite feedback...

8. Gendered Innovations: Why Does Sex of the Cell Matter?

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   ... requires that sex be included as a biological variable in all NIH-funded research. Janine Clayton, Director...

9. Plasmids 101: Golden Gate Cloning

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   ...Addgene’s plasmids are used with a wide variety of restriction enzyme-based cloning methods. Each method...

10. CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

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    ...Our paper shows we can easily make orthogonal variation. The first one worked in each case. Sometimes ...

11. Working with Nuclear Receptors

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    ...‘core’ complex is composed of a tightly-bound invariant group of proteins, whereas the more loosely bound...

12. Design Tips for Prime Editing

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    ...rereading many of the original papers reporting various prime editing tools. These papers are chock full...

13. Plasmids 101: The Wide World of Natural Plasmids

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    ...natural plasmids Natural plasmids often convey a variety of functions that allow their host organisms to...

14. Making CRISPR Plasmids Using Fragmid

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    ...Schematic depicting available destination vectors for various delivery methods, along with compatible inserts...

15. Rabies and Neuronal Tracing

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    ...ability to efficiently enter cells and deliver a variety of cargoes, genetically engineered neurotropic ...

16. Educational Planarian Tools for Teaching Developmental Biology Techniques

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    ... A. Elliott, Kimberly C. Tu, Alejandro Sánchez Alvarado, The American Biology Teacher, Vol. 79 No. 3, ...

17. Split-BioID: An Improved Method for Studying Protein-Protein Interactions

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    ...Find the BioID Plasmids Here! Macneill, S., & Varnaitė, R. (2016). Meet the neighbors: Mapping local ...

18. Lab Automation at Addgene

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    ...a table top and can be easily programmed for a variety of lab needs. Addgene uses it to fill as many vials...

19. CRISPR in the Clinic

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    ...transthyretin amyloidosis (hATTR) hATTR is caused by a variety of single nucleotide polymorphisms (SNPs) in the...

20. Viral Vectors 101: Virus Safety

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    ...accident-prone researchers. Note that safety policies will vary slightly from organization to organization, so you...