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... posts about viral vectors Subscribe to the Addgene blog Resources on Addgene.org Learn about our ready-to-use...

...used to target complementary DNA sequences. In laboratory settings, an sgRNA includes the crRNA and tracrRNA...itself, simplifying the CRISPR-Cas9 system for laboratory applications. At Addgene, we sometimes refer ...

...Additional resources on the Addgene blog Read more about optogenetics New to viral vectors? Browse our Viral...Viral Vectors 101 blog posts Learn more about precise targeted manipulation Resources on Addgene.org ...

...phagocytosis.  Other functions We’re still learning about Fc effector functions and all that antibodies can...isotypes and Fc receptors that we don’t know much about yet. Who knows what kinds of functions they may ...

...particular tasks, but they tend to be scattered about the internet. To help you keep your virtual molecular...other applications Primer design tools Many of the above-listed plasmid mapping tools have built-in primer...

...These days, it hardly seems like we finish writing about one dual CRISPR-transposon system before another...Programmable gene insertion in human cells with a laboratory-evolved CRISPR-associated transposase. Science...

...Molecular Biology Reference includes information about molecular cloning, how to choose a plasmid vector...straightforward as possible. If you have a question about a specific plasmid element that you would like answered...

...resources on the Addgene blog Read this blog post about genome-wide screening using CRISPR Find all of our...on Addgene.org Read our CRISPR guide Learn more about plasmid pooled libraries Find CRISPR plasmids for...

...Flame awardee Sophien Kamoun of the Sainsbury Laboratory in Norwich, UK: “We’re very pleased to distribute...Plasmid in 2015 Find Hot Plasmid Technologies Write about Your Favorite Plasmid Technology Resources on Addgene.org...

...on a treadmill. After being with the company for about six months, I learned there was a Genzyme-based ...Additional Resources on the Addgene Blog Learn about Addgene's Previous Work with Rare Disease Researchers...

...the same as not being a bad manager (see cartoon above – what type of bad manager have you had?). You can...Managing scientists I should add a few special words about managing scientists because, let’s face it, we are...

... plasmids you need. If you'd ever like to write about a recent plasmid deposit please sign up here.   ...emission maxima toward the red end of the spectrum (above 600 nm) and increasing the brightness. Arne Fabritius...

...the ABRC are here to support you! From questions about growing conditions, to guiding students through ...local science festivals, ABRC gets the word out about the importance of plant sciences. In 2017 alone,...

...Resources at Addgene.org Learn about Popular Plasmids in the Repository Learn about New Plasmid Technologies...

...vector name and serotype you’d like to be notified about. Controls Plasmid Serotype Name 114472  AAV5...vectors 101 eBook Resources on Addgene.org Learn about Addgene's viral service Find viral protocols and...

...last updated Nov 10, 2020. Now that we know all about antibiotic resistance genes, let’s consider another...the replicon. For example, the pMB1 ori maintains about 20 copies per cell, while pUC – which differs by...

...Our work accelerates and enhances productive collaborations as well as provides visibility for grant-driven...research is accelerating essential research in laboratories all around the world." We thank the Chan Zuckerberg...

... plasmids you need. If you'd ever like to write about a recent plasmid deposit please sign up here.  ...non-overlapping cells. Until now, tools for doing this were labor-intensive and required using manipulations such ...

... is difficult for new members of the lab or collaborators unfamiliar with these mutant plants. Using RUBY...resistant disease. (RUBY-Red: You can’t beet it!)   About the Author  Andrew Willoughby is a graduate student...

...naturally.  Sample Denaturing  First, let’s think about induced conformation changes. Several types of assays...immunoimaging assays. It can also elucidate information about what a given protein is doing or changing in different...