We narrowed to 841 results for: ang

1. Management for Scientists: Managing vs. Leading

   Type

   Blog Post

   ..., Snowball, Mask: How Leaders Spark and Sustain Change, by Peter Fuda and Richard Badham Leadership ...

2. 10 Ways to Share Your Science!

   Type

   Blog Post

   ... outreach. Here in the North Carolina Research Triangle area, scientists give talks and science cafés ...

3. Ligation Independent Cloning

   Type

   Protocol

   ...transformation/replication process. LIC employs long overhangs to form a stable association between fragments...exonuclease functions, T4 DNA polymerase can create overhangs of varying length (typically 10-12 bp) based on...recognition sequence. This can create multiple distinct overhangs with a single enzyme, and remove the restriction...in subsequent reactions. Step 3: Create Vector Overhangs Treat the linearized vector with T4 DNA polymerase...accomplished by gel purification . Step 5: Create Insert Overhangs Treat your purified PCR product with T4 Pol in...

4. HA Frankenbody, a New Imaging Tool to Visualize Single Molecules and Nascent Peptides

   Type

   Blog Post

   ...versatile tool that can be used to visualize a wide range of protein dynamics in vivo. In the future, this...

5. The Golden Stopwatch Award: Recognizing Facilitators of Research

   Type

   Blog Post

   ...material transfer agreement Technology Transfer: the exchange of intellectual property (as inventions, ideas...

6. Tetbow: Bright Multicolor Labeling for Neuronal Tracing

   Type

   Blog Post

   ...fluorescent proteins is only possible at low copy number ranges, resulting in low fluorescent protein level. The...

7. Fluorescent Biosensors for Measuring Autophagic Flux

   Type

   Blog Post

   ...for the measurement of autophagic flux, or the changes in autophagic activity, and are a more reliable...

8. Viral Vectors 101: What is a Virus (and a Viral Vector)?

   Type

   Blog Post

   ...Thankfully, through the use of engineering controls — changes made to the virus that eliminate or reduce the ...

9. Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative

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   Blog Post

   ...DNA staining dye Article contributed by Jennifer Tsang Figure 4: Double stranded DNA bound by tTALE-FP...

10. Meet the New Addgene Board Members!

    Type

    Blog Post

    ... Jennifer Tsang...

11. Celebrate Open Data Day with Addgene’s AAV Data Hub!

    Type

    Blog Post

    ... Angela Abitua...

12. Hot Plasmids June 2018 - Reverse transcriptase, nanobody, and protein-DNA interaction tools

    Type

    Blog Post

    ..., in turn, makes them more adaptable to a broad range of applications. Recently, the Spiess lab developed...

13. CRISPR Challenges: Standardization and Homology Directed Repair

    Type

    Blog Post

    ...resection and strand invasion are like a foreign language to me. But coming to #KSgenome and seeing the ...

14. Summer SciComm: Science Storytelling

    Type

    Blog Post

    ...one or two roadblocks that sparked creativity, changed the outcome of the project, or illustrates the ...

15. Science Careers: Unruly Interests Feed Many Paths

    Type

    Blog Post

    ...Science journalism programs at various universities range from week-long workshops to more in-depth master...

16. Addgene's Top 10 Blog Posts of 2015

    Type

    Blog Post

    ...Genome Edit Once you’ve used CRISPR/Cas9 to make a change to the genome, you need to check whether you’ve...

17. Plasmids 101: A Brief History of Plasmids and an Improved eBook!

    Type

    Blog Post

    ... wanting to study bacterial genetics. This all changed thanks, in part, to a collaboration initiated at...

18. Identifying Sequence Elements with SnapGene's Feature Database

    Type

    Blog Post

    ... sequence match even when the DNA sequence has changed. Identifying plasmid control elements Coding sequence...

19. The Strength of Story Telling

    Type

    Blog Post

    ...first author. When I raised this, he was pretty angry and started arguing with me. First I calmed the ...

20. Even more elegant: Single injection CRISPR/Cas9 in C. elegans

    Type

    Blog Post

    ...to Cas9 Sites in Caenorhabditis elegans. Paix A, Wang Y, Smith HE, Lee CY, Calidas D, Lu T, Smith J, Schmidt...