We narrowed to 956 results for: cha
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TypeBlog Post.... With your busy schedule, you may not have the chance to stop what you’re doing and make a phone call... Bryan Archambault...
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pSiM24: Simplifying Plant Genetic Engineering
TypeBlog Post...modified with duplicated enhancer domains and characterized in studies of tobacco, Arabidopsis, and corn...paper at Addgene. Other relevant blog posts 3 Challenges in Plant Synthetic Biology Quick, Versatile Plant... -
Mapping the 4D nucleome with CRISPR/Cas9
TypeBlog Post...inter- and intrachromosomal dynamics, and how they change in response to the cell cycle or other stimuli....intrachromosomal loci, a major benchmark in characterizing the 4D nucleome. Future modifications and applications... -
London Calling - Addgene’s New Europe Office in London
TypeBlog Post...States - Cambridge, MA to be exact. Today, that changes. Today, Addgene launches a brand new Europe office...scientists together from all over the world to exchange research materials in an easy way. She has a passion... -
New Viral Vectors - Fall 2024
TypeBlog Post...types of the basal ganglia. They work via newly-characterized enhancers that enable a whole host of (awesome...AAV-GfaABC1D-Cre-4x6T AAV5 Recombinases Stanley Thomas Carmichael New viral prep pAAV-EF1a-fDIO-Cre AAV5 Recombinases... -
CRISPR 101: Drug discovery and CRISPR
TypeBlog Post...model can be generated, and drugs screened for a change in pathology. If the disease pathology is complex...Target identification Not all diseases are fully characterized or have a simple pathology. In these cases, ... -
Addgene's a Nonprofit? Nonprofit Awareness Day 2017
TypeBlog Post... Mary, Max, Maya, Meghan, Melina, Meron, Michael Lo., Michael Le., Michel, Michelle, Morgan, Natashia,... -
SunTag and Fluorescent Imaging
TypeBlog Post...localized, and how its localization and expression may change across various conditions. However, this system...Improved brightness and signal-to-noise ratio Less chance of phototoxicity Reduced photobleaching Simplified... -
Plasmids 101: Positive and Negative Selection for Plasmid Cloning
TypeBlog Post... interest by taking advantage of bacterial characteristics such as prototrophy, the ability to synthetize...used for negative selection on 5% sucrose plates. Exchange of the sacB gene for the gene of interest allows... -
Five Questions to Ask If You're Deciding Between a Master's or PhD Program
TypeBlog Post...growth is possible even if your job title doesn’t change. Master’s level training can prepare you to present... career with a master’s. While it's possible to change fields of research with a PhD, a master’s is less... -
Viral Vectors 101: Parts of the AAV Packaging Plasmid
TypeBlog Post...Introducing changes and/or short peptides sequences into these variable loops is one method for changing a capsid... -
Oh, The Places You Can Go: Careers in Science Communication - Product Development
TypeBlog Post... a job and I stumbled upon eScience by luck, by chance, by complete happenstance. It was on Indeed or ...company. And make sure to subscribe to the Career Channel of the blog so you don’t miss the future posts ... -
Sleeping Beauty Awakens for Genome Engineering
TypeBlog Post...cells, but they are susceptible to cells’ defense mechanisms. The use of non-viral vectors, like transposons...sequences and then testing various combinations of changes. The basic functional version of the Sleeping Beauty... -
10 Ways to Share Your Science!
TypeBlog Post...people like a good story and science involves characters – in this case you – on a quest to ask and answer... Consider participating in a future SciFund Challenge. While the goal is ostensibly to get some funding... -
Advice for Choosing a Research Project
TypeBlog Post... apart a piece that can address a topic in a mechanistic way using the technology available.” Choosing...combining “new discoveries or technologies increase chances that your project will be fresh as well.” Don't... -
New Viral Vectors - Winter 2025
TypeBlog Post...Optogenetics Gordon Fishell New viral prep pAAV_BiCHATe27_dTomato_nlsdTomato AAV-PHPeB Control Gordon Fishell New... New viral prep pAAV_BiCHATe27_ChR2_mCherry AAV-PHPeB Optogenetics Gordon Fishell New viral prep pAAV_BiVIPe4... -
Viral Vectors 101: Transductions
TypeBlog Post...involve DNA incorporation into the cell, but the mechanisms vary. Why transduce? So why use a virus to deliver...transduction Transductions utilize the ‘normal’ mechanism of viral infiltration into a cell. The twist is... -
Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools
TypeBlog Post...LOV2 and Zdk1 to dissociate due to conformational changes, and as a consequence, EB1 protein function is ...expansion of the genome editing toolkit. However, a challenge in optimizing base editing technologies across... -
Hot Plasmids June 2018 - Reverse transcriptase, nanobody, and protein-DNA interaction tools
TypeBlog Post...antibody fragments typically engineered from heavy-chain only antibodies found in camelids. They possess ...H2O2, APEX2 labels electron-rich amino acid side chains (e.g. Tyrosine) with biotin. These biotin-labeled... -
Arabidopsis in Education: How the Arabidopsis Biological Resource Center Brings Plant Science to Life
TypeBlog Post...more. Downloadable materials are available free-of-charge from the ABRC Outreach website. Seeds, which are...system comes with fewer ethical and logistical challenges than does working with animals. Because Arabidopsis...