We narrowed to 952 results for: cha

... Mary, Max, Maya, Meghan, Melina, Meron, Michael Lo., Michael Le., Michel, Michelle, Morgan, Natashia,...

...model can be generated, and drugs screened for a change in pathology. If the disease pathology is complex...Target identification Not all diseases are fully characterized or have a simple pathology. In these cases, ...

...types of the basal ganglia. They work via newly-characterized enhancers that enable a whole host of (awesome...AAV-GfaABC1D-Cre-4x6T AAV5 Recombinases Stanley Thomas Carmichael New viral prep pAAV-EF1a-fDIO-Cre AAV5 Recombinases...

... interest by taking advantage of bacterial characteristics such as prototrophy, the ability to synthetize...used for negative selection on 5% sucrose plates. Exchange of the sacB gene for the gene of interest allows...

...localized, and how its localization and expression may change across various conditions. However, this system...Improved brightness and signal-to-noise ratio Less chance of phototoxicity Reduced photobleaching Simplified...

...cells, but they are susceptible to cells’ defense mechanisms. The use of non-viral vectors, like transposons...sequences and then testing various combinations of changes. The basic functional version of the Sleeping Beauty...

...people like a good story and science involves characters – in this case you – on a quest to ask and answer...   Consider participating in a future SciFund Challenge. While the goal is ostensibly to get some funding...

... apart a piece that can address a topic in a mechanistic way using the technology available.” Choosing...combining “new discoveries or technologies increase chances that your project will be fresh as well.” Don't...

...LOV2 and Zdk1 to dissociate due to conformational changes, and as a consequence, EB1 protein function is ...expansion of the genome editing toolkit. However, a challenge in optimizing base editing technologies across...

...antibody fragments typically engineered from heavy-chain only antibodies found in camelids. They possess ...H2O2, APEX2 labels electron-rich amino acid side chains (e.g. Tyrosine) with biotin. These biotin-labeled...

...level of independence, such as museums, YouTube channels and podcasts, science pub nights, and pop sci ...An audience can be defined by any number of characteristics. Common ones include age range, familiarity...

... positive plants, but screening these can be challenging and requires specialized equipment. Reporters...Department at the University of North Carolina at Chapel Hill. He has a passion for microscopy and beautiful...

...more. Downloadable materials are available free-of-charge from the ABRC Outreach website. Seeds, which are...system comes with fewer ethical and logistical challenges than does working with animals. Because Arabidopsis...

...anecdotal forms of information — identifying with a character, building emotional investment and following cause-and-effect... to date, invite people to ask questions, be approachable. Engage with your audience and try to learn ...

...particles containing complementary polynucleotide chains. J Virol 9(2): 394-396. Lecomte E, Tournaire B,...Redon R, Moullier P, Léger A (2015) Advanced Characterization of DNA Molecules in rAAV Vector Preparations...

...our growing group of Addgene AAV data sharing champions? If you’ve recently used Addgene AAV tools, we...caption. For microscopy images, please include channel imaged, a color legend for probes/dyes/labels, ...

...the success of the change in expression of GFP, for the stop codon should have changed from “TGA” to “TGG...

...which are more likely to carry the full plasmid. Chakiath & Esposito (2018) noticed that in Stbl3 bacterial.... https://doi.org/10.1007/s13205-012-0070-8   Chakiath CS, Esposito D (2007) Improved recombinational...

...Optogenetics Gordon Fishell New viral prep pAAV_BiCHATe27_dTomato_nlsdTomato AAV-PHPeB Control Gordon Fishell New... New viral prep pAAV_BiCHATe27_ChR2_mCherry AAV-PHPeB Optogenetics Gordon Fishell New viral prep pAAV_BiVIPe4...

...involve DNA incorporation into the cell, but the mechanisms vary. Why transduce? So why use a virus to deliver...transduction Transductions utilize the ‘normal’ mechanism of viral infiltration into a cell. The twist is...