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We narrowed to 911 results for: ire;

Showing: 661 - 680 of 911 results

  1. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    ... inhibitor response 1 (TIR1) expressed under a desired promoter with a specific spatiotemporal expression...motif (PAM). They're used to determine the PAM requirements of Cas enzymes. CRISPR-ARE is a new system from...

  2. 6 Tips for Grant Writing

    Type
    Blog Post
    ...Just like with any sport, hours of practice are required and no one lab or professor becomes an overnight...they are looking for in grant submissions and will direct you on how to submit your work either through seminars...

  3. Adenoviral Vector Production and Troubleshooting

    Type
    Blog Post
    ...Therefore, co-infection with a helper adenovirus is required to provide the viral proteins in trans. The Gutless...virus. Purification (2 days) - Purification is required if rAdV is to be used in vivo. The standard method...

  6. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type
    Blog Post
    ...genotype each animal to identify the ones with the desired genotype. Inducible and conditional systems Conditional...conditional knockout mouse, loxP sites are inserted directly into the genome to flank the gene that will be...

  7. Hot Plasmids - October 2020

    Type
    Blog Post
    ... elegans with CRISPR can be difficult since it requires the much less efficient double stranded templates...cell cycle. Cells that had IL-3 withdrawn became entirely red as they could not proliferate. Eastman et...

  8. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    ...capabilities. Specifically this includes primer directed isothermal RNA amplification, new toehold switch...samples; we showed that we can detect the virus directly from infected monkey blood plasma without purification...

  11. Hot Plasmids: Winter 2025

    Type
    Blog Post
    ...Optimized dicot prime editing enables heritable desired edits in tomato and Arabidopsis. Nature Plants,..., M. D., Wu, J. C., & Garcia-Marcos, M. (2024). Direct interrogation of context-dependent GPCR activity...

  14. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    .... A special class of FPs, the FP timers, add an entire new dimension to monitoring localization; using...emission and absorption spectra). Subach et al’s directed mutagenesis also produced the three separate timers...

  15. Tips to Make the Most of a Scientific Conference

    Type
    Blog Post
    ...poster: those that want you to walk them through the entire poster, or those that want to look through themselves...handle on your nametag for the tweep up (and for the entire conference, in general). After the conference Review...

  16. Anatomy of a Plasmid Page at Addgene

    Type
    Blog Post
    ...in the depositing lab, although you may have an entirely different use in mind! This section of our plasmid...in the E. coli lab strain DH5a, but others may require strains such as ccdB survival (for Gateway plasmids...

  19. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    ...emission is green or red light. For example, T-Sapphire, LSSmOrange, and LSSmKate. Fluorescent Sensors...many FPs (particularly those derived from GFP) requires oxygen. Therefore, these FPs cannot be used in...
Showing: 661 - 680 of 911 results