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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 921 results for: ire;

Showing: 661 - 680 of 921 results

  1. Seven Tips for Using LinkedIn as a Scientist

    Type
    Blog Post
    ...those planning a career in academia, it may not be required, but many academic scientists are starting to ...use LinkedIn messaging, not everyone likes to. A direct email can be the best way to get in touch if you...

  5. Hot Plasmids - October 2020

    Type
    Blog Post
    ... elegans with CRISPR can be difficult since it requires the much less efficient double stranded templates...cell cycle. Cells that had IL-3 withdrawn became entirely red as they could not proliferate. Eastman et...

  6. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    ... inhibitor response 1 (TIR1) expressed under a desired promoter with a specific spatiotemporal expression...motif (PAM). They're used to determine the PAM requirements of Cas enzymes. CRISPR-ARE is a new system from...

  7. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type
    Blog Post
    ...genotype each animal to identify the ones with the desired genotype. Inducible and conditional systems Conditional...conditional knockout mouse, loxP sites are inserted directly into the genome to flank the gene that will be...

  8. Hot Plasmids February 2024

    Type
    Blog Post
    ...for your next experiments, they will also likely inspire further-improved versions. Thanks to Christophe...Leterrier, whose FocalPlanes piece on mStayGold inspired and informed this section.  Find mStayGold plasmids...

  9. Adenoviral Vector Production and Troubleshooting

    Type
    Blog Post
    ...Therefore, co-infection with a helper adenovirus is required to provide the viral proteins in trans. The Gutless...virus. Purification (2 days) - Purification is required if rAdV is to be used in vivo. The standard method...

  11. Design Tips for Prime Editing

    Type
    Blog Post
    ...reverse transcriptase (RT) template including the desired edit. Figure 1: Prime Editing schematic....guide Find CRISPR plasmids by function Browse our entire plasmid collection ...

  12. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

    Type
    Blog Post
    ...functional loss (Ho et al. 2014). To overcome this, paired gRNAs can be used to target specific sizes of a...visualizing ncRNA localization and function, but directly incorporating very long ncRNAs is impractical....

  13. Antibodies 101: Stripping and Reprobing Western Blots

    Type
    Blog Post
    ...antibody affinity: stronger binding antibodies will require harsher stripping to remove, so start with the ...with caution. Unlike PVDF, they can be returned directly to buffer without a re-wetting step. Important...

  15. 6 Tips for Grant Writing

    Type
    Blog Post
    ...Just like with any sport, hours of practice are required and no one lab or professor becomes an overnight...they are looking for in grant submissions and will direct you on how to submit your work either through seminars...

  16. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    ...capabilities. Specifically this includes primer directed isothermal RNA amplification, new toehold switch...samples; we showed that we can detect the virus directly from infected monkey blood plasma without purification...
Showing: 661 - 680 of 921 results