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We narrowed to 971 results for: lars

Showing: 661 - 680 of 971 results

  1. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    ...moist and hopefully aerated with a thin layer of molecular biology-grade agarose containing physiological... advantage of this technique is the potentially large number of single clones obtained (on occasion we...Maryland Medical School in Baltimore. She is particularly interested in signaling peptide maturation in...

  2. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    ...see if any other fluorescent proteins display a similar effect (many do). Fusing EGFP to the self-labeling... tools, they’re optimistic that MagLOV can be similarly adaptable into future magnetogenetic tools.  Embracing...passive impact,” he says, noting that his team regularly shares troubleshooting tips and works with other...

  3. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    ...editor experimentalist, and especially shines once large-scale prime editing experiments come into play. ...Kissling, L., Marquart, K. F., Schmidheini, L., Solari, C., Balázs, Z., Krauthammer, M., & Schwank, G....DNA double-strand break repair pathway balance. Molecular Cell, 81(10), 2216-2230.e10. https://doi.org/10.1016...

  4. Academic vs. Industry Postdocs

    Type
    Blog Post
    ...drug to the market usually takes over a billion dollars and at least a decade of work. The number of steps... Where publication is expected, the process is similar to academia. The only difference is that your paper...could be laid off while you’re there. Of course, similarly, an academic advisor could also lose their funding...

  5. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    ...cultures with intact ITRs. I use a spreadsheet similar to this one to plan my AAV packaging PEI transfections... This helps avoid multiple freeze/thaws of your larger aliquots of virus. AAV titration by qPCR Overview...in vitro and in vivo use. Current protocols in molecular biology, Chapter 16, Unit 16.25. PubMed PMID: ...

  6. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    ...Cas9 technology has revolutionized the fields of molecular biology and bioengineering, as it has facilitated...gRNAs Importantly, the use of truncated guides has largely been demonstrated to result in decreased mismatch...technologies. To date his group has develop several popular CRISPR-based tools for altering DNA sequence or...

  7. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    ...figure below). The double-MMEJ strategy looks very similar to HDR, but it is accomplished using much smaller... of MMEJ. This double MMEJ strategy looks very similar to HDR, but it is completed using much shorter ...whole-plasmid integration if you’d like to integrate a larger amount of material into the genome. Open questions...

  8. Summer SciComm Series: Modes of Communication

    Type
    Blog Post
    ...beautifully compelling photos and videos, while molecular, micro, and cell biology are often more suited...microscopy) Visually compelling. Easy to convey a large number of details without overwhelming your audience...Difficult to do in-depth storytelling. Requires regular content production. Internet "trolls"   More...

  9. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

    Type
    Blog Post
    ...Addgene, as a proxy for global activity in our particular scientific subfields.  Plasmid requests correlate...plasmid requests correspond to publications of popular papers. Figure 1: Plasmid requests by date...-examine our global data at a finer temporal granularity. For each paper, we observed that the demand ...

  10. An Introduction to Adenovirus

    Type
    Blog Post
    ...hexon), as well as other intracellularly-exposed proteins and downstream cellular events, all recruit strong...Sarvetnick, N., Sherman, L., & Verma, I. (1998). Cellular immune response to adenoviral vector infected ...

  11. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    ...generating a frameshift mutation. The majority of cellular repair events will generate these types of mutations...hundred bps of sequence. However, if you have a larger knock-in (200 bp – 2 kb), a dsDNA donor will need...oligonucleotides. These donors traditionally have larger homology arms in the 500 bp to 1 kb range, however...

  12. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

    Type
    Blog Post
    ...journal articles. From paper to plasmid Authors regularly indicate that their plasmids are available from... very likely find a useful plasmid, but what’s popular? The coveted Blue Flame is more than an award for... Tags: Identify a specific marker protein Use: Similar to “Vector Type” you can filter by experimental...

  13. CRISPR Antimicrobials

    Type
    Blog Post
    ...vectors are replication-deficient. Thus, a very large number of phagemids would be needed for an in vivo...Diana P. Pires, and Timothy K. Lu. “Engineering Modular Viral Scaffolds for Targeted Bacterial Population...Methods for Bacterial Genome Engineering Treating Muscular Dystrophy with CRISPR Gene Editing Using CRISPR...

  14. Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

    Type
    Blog Post
    ... being used. After learning of it's continued popularity, I decided this protocol would be a worthy first...Addgene has also cataloged a variety of useful molecular cloning protocols, including making LB agar plates...needs. At Addgene, we’ll continue to create new molecular biology educational resources, such as protocols...

  15. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    ...are not always positioned correctly to target a particular gene. Furthermore, a target sequence may have... desired target locus. The PAM sequence is of particular concern when trying to edit a gene using homology...cleavage events for the VQR and VRER variants is similar to wild-type SpCas9, indicating that the variants...

  16. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    ...known to have replicons with broad host ranges. Popular replicons from these groups include: RK2 (IncP)...vectors for gene expression in bacteria. Methods in Molecular Biology (Clifton, N.J.), 765, 327–343. https:/...endonucleases on the broad host-range plasmid RK2. Molecular & General Genetics: MGG, 152(3), 129–135. https...

  17. Plasmids 101: Control Plasmids

    Type
    Blog Post
    ...level of Gene X in cells treated with Plasmid A is similar to the expression level of Gene X in the untreated... controls are negative controls that produce a similar product, but do not target an endogenous gene in... variable and helps ensure the phenotype isn’t singular or associated with only one aliquot/preparation...

  18. Protocol - How to Run an Agarose Gel

    Type
    Protocol
    ... the gel in the first place. Carefully load a molecular weight ladder into the first lane of the gel. ...you are conducting certain procedures, such as molecular cloning, you will need to purify the DNA away ...you get better separation of bands? If you have similarly sized bands that are running too close together..., and a lower percentage gel will help separate larger bands. 10% Rule For each sample you want to load...

  19. What is Polymerase Chain Reaction (PCR)

    Type
    Protocol
    ...cytosine (C), guanine (G), thymine (T)] in an equal molar ratio. It also requires two unique single stranded...copy the DNA in between the oligo primers. This molecular biology technique creates several micrograms of...Your 5’ and 3’ primers should be designed to have similar melting temperatures (Tm). Set the annealing temperature...frozen. DMSO helps with denaturation of the DNA, particularly for GC-rich templates. What does each ingredient...

  20. Tips for Getting a Faculty Position

    Type
    Blog Post
    ... matter how many times I say this, candidates regularly try to convince me that their search committee...whiskies, but I remember the days of modest postdoc salaries all too well. If there's a group to make money...search!   Erik Lee Snapp received his PhD in Molecular Microbiology and Immunology from Oregon Health...
Showing: 661 - 680 of 971 results