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We narrowed to 931 results for: por

Showing: 661 - 680 of 931 results

  2. Plasmids for Endogenous Gene Tagging in Human Cells

    Type
    Blog Post
    ...lines can be found in the Cell Catalog on our data portal. The plasmids should be functional in many different...and the donor plasmid into the cells we use electroporation, a technique that briefly destabilizes the ...

  4. Hot Plasmids - October 2020

    Type
    Blog Post
    ...grown in high concentrations of IL-3 show a high proportion of blue fluorescence, indicating cell proliferation...they could not proliferate. Eastman et al. Cell Reports 2020. https://doi.org/10.1016/j.celrep.2020.107804...

  7. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    ...antibodies for IHC. Working with collaborators and support from the Brain Research Through Advancing Innovative...recombinant antibodies are a newer technology, it is important to show through qualitative data that they are...

  9. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    ... reactive ones. And make sure to check for any reported cross-reactivity when planning your experiment...Why Choosing the Right Secondary Is of Primary Importance. In J. Sturtevant (Ed.), PLoS ONE (Vol. 7, Issue...

  10. Early Career Researcher Toolbox: Finding Relevant Papers

    Type
    Blog Post
    ...about publications in the fields of research they support. Monitoring these types of accounts can help you...and figures using machine learning to extract important features such as influential citations, images...

  11. How-to: 5 Steps to a Great Panel Discussion

    Type
    Blog Post
    ...so her ability to control the event is what is important. Choose 4 panelists (maybe 5) and no more. Too... members before a panel discussion and often incorporate them into the program. I have to admit to inviting...

  12. Transfection for Recombinant Antibodies

    Type
    Protocol
    ...500ML Benzamide, Millipore Sigma 12072 Antipain, Millipore Sigma 10791 Leupeptin, Millipore Sigma L2884 Aprotinin...that sharing the full details of our protocols supports reproducibility and accelerates science. Here,...information is solely for transparency intended to support reproducibility in science. General Considerations... Aprotinin saline solution, Millipore Sigma A6279 Before Starting Warm the DNA and working stock of PEI-MAX...

  15. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    ...area (the amount of agarose/DMEM should be proportionately increased). For some cells, add a drop of serum...antibiotics. Lastly, and perhaps obviously, it is important to have a functional assay in place to screen ...

  16. Polymerase Chain Reaction Overview and Applications

    Type
    Blog Post
    ... a fluorescent signal. The signal increases proportionally to the amount of product produced each cycle...multiple starting DNA templates in one sample. It is important to note however, that multiplex PCR is more complicated...

  17. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    ...of production, saving about a week of work. An important note is that this is only possible because baculoviruses... will come down to which differences are most important to you — virus type, cost, time, or reagents and...

  18. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    ... In our study, we used the TRIP (Thousands of Reporters Integrated in Parallel; Akhtar et al., 2013; Schep...Chromatin Position Effects Assayed by Thousands of Reporters Integrated in Parallel. Cell, 154(4), 914–927....

  19. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    ...cells that have randomly integrated all or large portions of the plasmid. Deliver your construct to your...marker, but cells with random recombination may incorporate the negative selection marker into the genome...

  20. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    ...technique can be adapted to other transgenes. It’s important to note that the fluorescence level observed will...to insert cDNA constructs into this locus. One important question to ask is how the efficiency of PITCh-based...
Showing: 661 - 680 of 931 results