We narrowed to 922 results for: tro

1. A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells

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   ... editing effects. ABEs have been described to introduce base changes in cellular DNA and RNA dependent...role for off-target editing effects. ABEs can be introduced into cells as a plasmid or as mRNA constructs...

2. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

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   ...favorite gene. But have no fear - I’m here to introduce you to a simpler, yet more effective, CRISPR-based...Ming G-L, King JR, Song H, Sweatt JD (2013) TET1 Controls CNS 5-Methylcytosine Hydroxylation, Active DNA...

3. Cpf1 Update: Comparison to Cas9 and NgAgo

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   ... easiest to adapt to genome editing, but the introduction of type V Cpf1-driven systems added another ... endjoining (NHEJ). Cas9-mediated NHEJ usually destroys the PAM site due its proximity to the cleavage...

4. Plasmids 101: Modular Cloning

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   ...Interview with Nicola Patron on MoClo Plasmids 101: Golden Gate Cloning ... Susanna Stroik...

5. Progress Towards a PAM-Free CRISPR

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   ...want to knock out a gene, a frameshift mutation introduced anywhere within the early coding region will ...  Susanna Stroik...

6. 7 Simple Strategies to Resolve Conflicts with Difficult Supervisors

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   ...who spend most of their time moaning about their troubles, Silent or unresponsive clams, who seem to ignore...Indecisives, who keep changing their minds and have trouble making decisions. In my work as a thesis coach...

7. Production of Virus in Insect Versus Mammalian Cells

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   ...ll need to produce your viruses in-house.   Introducing the systems There are many ways that viruses ...insectspodoptera frugiperda (lepidoptera; noctuidae). In Vitro, 13(4), 213–217. https://doi.org/10.1007/bf02615077...

8. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

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   ...research moving. Below, you’ll find some great introductory resources. As you dive deeper into Drosophila...TRiP- Functional Genomics Resources website has in vitro reagents and tools to help you knock down, knock...

9. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

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   ...apart is the way they generate their color: they strongly absorb visible light to give colors in ambient...genome, it is not expressed as it is under the control of a rhamnose-inducible promoter. The resolution...

10. Tips for a 1st time AAV user (by a Rookie AAV user)

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    ...not that I would know about this… A no template control (NTC) should always be included. While the physical...recombinant adeno-associated viral vectors for in vitro and in vivo use. Current protocols in molecular ...

11. 3D Printing Meets CRISPR Cas9

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    ...protein’s structure into experimentally determined electron density, have been known to say something silly...educators. Using these hands-on tools, educators introduce their students to this otherwise invisible molecular...

12. Fluorescent Tagging of Endogenous Genes with SapTrap

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    ...used to tag endogenous genes and simultaneously introduce a selection marker for screening modified strains...with a Cas9 expression vector. They can also be introduced directly into a Drosophila S2 cell line stably...

13. When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

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    ...Importantly, all of these assays are performed in vitro. Assays to detect FP dimerization in in vivo cellular...Structural Biology. His interests include the quality control of secretory proteins in the Endoplasmic Reticulum...

14. Light Sheet Fluorescence Microscopy

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    ...image acquisition is performed using cameras with electron multiplying couple-charged device (EMCCD) or complementary...use of viruses allows much better spatiotemporal control over labeling methods as compared to transgenic...

15. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

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    ...can occur in two different orientations, but is strongly biased towards T-RL, where the R end is proximal...coli (Klompe et al., 2019). Recently, the lab introduced a streamlined expression approach (Fig. 2A), ...

16. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

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    ...False, embed_button=False, autoplay=False, hidden_controls=False, loop=False, muted=False, width='600'... impacted. The inferred weekend effect is, in retrospect, generally more dramatic than the COVID-19 effect...

17. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

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    ...anyone can find enhancers with Quilt. Seek and destroy enhancers with CRISPR Armed with the genome math...ESCs) with a lentiviral vector, an attenuated retrovirus in the same family as HIV. By design, our lentivirii...

18. DNA Purification Without a Kit

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    ...but the addition of a high concentration of a chaotropic salt (guanidine hydrochloride for the plasmid... glass milk, gel slices are first placed in a chaotropic salt sodium iodine (NaI) solution. NaI has two...

19. Tips for arabidopsis transformation

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    ...like to isolate a minimum of five. You have no control over how many insertions occurred. Having too many...segregate 3:1 in the T2 generation. You also have no control over where your construct will be inserted. If ...

20. Cpf1: A New Tool for CRISPR Genome Editing

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    ... easiest to adapt to genome editing, but the introduction of type V Cpf1-driven systems has added another... endjoining (NHEJ). Cas9-mediated NHEJ usually destroys the PAM site due to its proximity to the cleavage...