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We narrowed to 1,010 results for: RING;

Showing: 341 - 350 of 1010 results

  1. Common Injection Routes in Mice

    Type
    Blog Post
    ... the abdomen. They are a popular choice for administering different drugs and viral vectors. Like IV injections...suggests, in the muscle. Common uses include administering vaccines, pain meds, or other drugs. Identifying...don’t always require needles. If needles make you cringe or your experiment will allow you to avoid poking...straight to the stomach. This technique involves administering medication through a feeding tube with a bulb...introduction to common injection routes in mice! When considering what method you’d like to use, remember that ...Organisms Additional resources on Addgene.org Genome Engineering Guide CRISPR Mammalian Expression plasmids Cre-Lox...

  2. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    ...tissue clearing Similar to light microscopy, there have been significant advances in tissue clearing techniques...brightness with increasing depth caused by light scattering and absorption.  Finally, the photomultipliers...techniques during this century.  The first successful attempt to render fixed anatomical preparations transparent...Ultramicroscopy with tetrahydrofuran-based tissue clearing (13,14). A few years later, Karl Deisseroth’s ...  The combination of LSFM with multiple tissue clearing strategies and neuronal labeling methods will ...

  3. Transferable Skills Guide: Identifying Your Transferable Skills

    Type
    Blog Post
    ...held. Imagine this situation: You’ve spent hours scouring a company’s LinkedIn pages, given yourself the...guaranteed to get the job, however, many times the hiring team will also want to examine not just technical...technical skills, but also transferable skills, before offering someone their dream job; They want to know if ... and to demonstrate them to the people who make hiring decisions. Though having all the technical skills...get a great snapshot of the skills that you can bring into pretty much any other workplace. To get your... for non-academic science careers Learn about mentoring Resources on Addgene.org Check out our careers...

  4. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    ... parents in STEM careers, chances are they’re wondering how they could contribute to the school, too. ...Middle school: supplementing the science curriculum Sharing my expertise with our district middle school was... an authority in this area. What is genome engineering? How does CRISPR work? This section was essentially... of the talk by running it by the teachers and sharing some slides on Twitter, where I was able to get...computers and the internet before it, genome engineering technology is moving along much faster than it...understanding genetics is so timely.” Genome engineering, and many other technologies and fields in biology...

  5. Getting to Know Expansion Microscopy

    Type
    Blog Post
    ...by loss of protein during digestion, or masking of epitopes during sample anchoring and gel polymerization... Tillberg, et al. 2016). That being said, the anchoring, digestion, and expansion steps can impact the...occurs, due to a reduction in target concentration during expansion. One group has found that using Fab fragment...et al (2023) Magnify is a universal molecular anchoring strategy for expansion microscopy. Nat Biotechnol...

  6. MXS Chaining

    Type
    Blog Post
    ...Knight TF. Engineering BioBrick vectors from BioBrick parts. Journal of biological engineering. 2008;2:5... fluorescent protein were fused in-frame to a tethering partner, which directed localization of the fluorophore...Construct Fluorophore Excitation/emission maxima Tethering partner Subcellular localization 1 TagBFP 399nm...Constraints on the order of the modules, because neighboring fragments require compatible cohesive ends Engler...biological part assembly. Journal of biological engineering. 2010;4(1):1. Pubmed PMID: 20205762. PubMed Central...

  7. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

    Type
    Blog Post
    ...the non-radiative transfer for energy between neighboring fluorophores, FRET can be used to detect the ...ratios) to generate useable data. What’s more, measuring and quantifying FRET is often easier said than...complete FP because the PIPs they’re tethered to bring them into close proximity (Figure 1). Formation ...independent of PPIs. Overexpression can be assessed by comparing the expression levels between BiFC constructs ...Analysis of Protein Interactions in Live Cells. Cold Spring Harb Protoc 2013:pdb.prot076497. https://doi.org...Fluorescent Protein 101 blog posts Read our blog post covering an introduction to FRET Resources on Addgene....

  8. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    ...where droplet digital PCR (ddPCR) comes in. AAV titering using quantitative PCR Before we dive into the... PCR is complete, the results are analyzed by comparing the threshold fluorescence of the viral DNA to...reasons, we are in the process of transitioning our titering method from qPCR to ddPCR. Droplet digital PCR...reference, meaning saving on reagents (and time)! AAV titering using droplet digital PCR Droplet digital PCR ...generator, you will have to be careful about transferring your droplets from the droplet generator to ...detection of microbes. Some applications include measuring viable probiotics and detecting circulating pathogens...

  9. Save Time with Transient Plant Leaf Transformations

    Type
    Blog Post
    ...are syringe or vacuum infiltration. Syringe infiltration (D'Aoust et al., 2009): Usually a syringe without...contains a plasmid with a T-DNA (transfer-DNA) region. During infection this T-DNA region is transferred into...transformation and gene expression can be tracked. During a transient leaf transformation, the engineered...Transient expression of antibodies in plants using syringe agroinfiltration." Recombinant proteins from plants...

  10. Designing Your Chalk Talk for the Academic Job Interview

    Type
    Blog Post
    ... You need to balance respectfully answering questions while steering the conversation back to your vision...proposal or a 10-year research plan. I suggest preparing for both types. Your research vision is your approach...proposal will distinguish between competing models. Comparing model A vs. B is more constructive than testing...vision. On the day of your chalk talk Bring your own fresh set of erasable markers. You’ll have the colors...and consider eating one before your chalk talk. Bring your own bottle of water. If possible, write down...
Showing: 341 - 350 of 1010 results