We narrowed to 960 results for: comp

...fluorescent proteins, how to do a head-to-head comparison of fluorescent proteins and how to make a well-informed...your selection on a small-scale head-to-head comparative study under conditions that best mimic the intended...protein properties and provides a quick way to compare fluorescent proteins, e.g. see Chudakov et al (...is of no use. Therefore, it is more sensible to compare fluorescent proteins based on their practical brightness...promising fluorescent proteins and to determine and compare their practical brightness in a system closely ...application. In figure 2 we provide an example of the comparison of the practical brightness of two orange fluorescent...

...expressed in cells, generates much less signal than comparable fluorescent proteins. This weak emission hasn... with increased sensitivity and faster imaging compared to previous publications. They also developed ...they successfully imaged multiple subcellular compartments over several minutes using Nano-lantern fusion... fluorescence, so higher concentrations of the compound can be added to the media. Cellular esterase then...producing a constant supply of substrate. Using this compound to excite the Nano-lanterns, Takai et al. continuously... handy. This approach is especially suited to complement optogenetic tools, where light used for excitation...

...and reduce needed compensation.    Working with antibodies can get a bit complicated, but hopefully this...time-sensitive, using precious samples, or being compared to other data in a manner that demands high accuracy... reaction to understand if any of your buffer components could affect the reaction. For instance, sodium...detected in the same assay. This is one of the most complex subjects in antibody-based protocols - but thankfully...another channel's detection range. Though you can compensate for this when reading your data, you’ll want ...

...all bacteria are naturally competent to take up DNA, they can be made competent through chemical manipulation...Addgene’s blog to learn about making your own competent cells and our protocols page to learn about bacterial... The phagemids are packaged into replication-incompetent phage particles with assistance from a ‘helper... A., and Karen Guillemin. "Multiple phases of competence occur during the Helicobacter pylori growth cycle...Meibom, Karin L., et al. "Chitin induces natural competence in Vibrio cholerae." Science 310.5755 (2005):...

...track of computation work comes with new sets of challenges. One challenge with computational analyses... of biologists are finding themselves using computational approaches to analyze large data sets (myself...RNA-seq analysis. I had never interacted with a computer at the command line, and so was really starting...especially since you’ll want to dig in to the computation, but they will serve you well as you perform ...directory: A directory is a file system in the computer and is just another name for a folder. For instance...

...increased versatility in probe attachment, and compatibility with far-red and near-infrared fluorophores....enable highly specific visualization of cellular components, typically a protein of interest. Fluorescent...ligands Effects on protein function and cellular components: It's important to assess how dye-conjugation... function and its propensity to bind cellular components. Some dyes target specific organelles like mitochondria...may prove challenging to stain intracellular compartments in in vivo model systems, so extra care must...Transfer (FRET) applications, the dyes need to have compatible excitation and emission profiles to enable effective...finding the right dye for your experiment more complicated. We hope this post helps you in your search for...

...presentation, lost in a sea of data, my mind went completely blank. After several seconds of truly panicked...honest, if my paper hadn’t been ridiculously more complex than the one before it, I don’t think I would have... panicked state, only option one would let me accomplish my goal.   As a scientist, public speaking isn't...dumpster noises were just last week!  Nerves It’s completely normal to be anxious, nervous, and/or apprehensive... in its most difficult form. You’re presenting complex data, to a group of peers who are there to provide...conferences, or even just provide quick updates during a company meeting increases visibility of your work, opens...

...based on size. Whether higher molecular weight complexes are detected will depend on the homodimer affinity...During the experiment, the solution is diluted, complicating the interpretation. Because of this, these methods...fluorescent protein is monomeric without the accompanying data to prove it is worthless. The OSER assay...and biological properties of new fusions can be compared with mEGFP or mTurquoise2, which are established...that you will be using. If you do a head-to-head comparison right, it will be valuable information for the... are engineered can be shared to simplify the comparison by others. Together, we can build a valuable ...

...us to the key question: what is the best way to compare the photostability of different fluorescent proteins...measurements for different fluorescent proteins and by comparing how the fluorescence intensity changes over time...photostability of the fluorescent proteins can be directly compared. An example of a photostability measurement of...fluorescent protein can be identified by a head-to-head comparison of several fluorescent proteins of the same spectral.... In figure 1 we provide an example of such a comparison between cyan fluorescent proteins using widefield... power, the photostability of the variants is comparable (lower panel of figure 1). To avoid changes in...

... and trolls. People don’t like to connect with complete strangers as it borders on my first rule of networking...’m asked a lot about requesting connections to complete strangers for informational interviews. Many have...option is in the “More…” menu. You can also follow companies and organizations to see their news and job postings...interviews.  Pro tip: You can learn a lot from the company LinkedIn page. This can include news, inclusion...It’s also a great way to keep track of past accomplishments. Follow all the rules of good resume writing...writing. Include examples of concrete accomplishments in both the technical and “plus skills” areas. Include...