Skip to main content
Addgene
Showing: 681 - 700 of 950 results

  1. Plasmids 101: Yeast Vectors

    Type
    Blog Post
    ...Instead, the size and A-T content of the DNA (apparently independent of a known specific sequence) dictate...non-physiological levels. Scientists have tried varied approaches to combat these issues. One method to reduce ...

  2. Troubleshooting Your Plasmid Cloning Experiment

    Type
    Blog Post
    ...sequence has high GC or AT content, increase the overlapping region of the primer to 40-60 bp. It is always...these steps, you might want to reconsider your approach, and try a different cloning strategy. Once you've...

  3. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    ...MAGIC plasmids here Paper-based synthetic biology approach to analyze gut microbiota and host biomarkers ...standards, the assay is semi-quantitative and can approximate mRNA concentration within a sample. Using this...

  4. Lighting Up Cell Signaling with Photoswitchable Kinases

    Type
    Blog Post
    ...spatiotemporal resolution than pharmacological or genetic approaches, but only a few such methods exist and they only...kinases good for? There are a number of potential applications for photoswitchable kinases.  Below are a few...

  6. Summer SciComm Series: Cognitive Load

    Type
    Blog Post
    ...physicist, etc., is an immense task (it takes, ooh, approximately the length of your average PhD to achieve!) ...not communicating as clearly as you intended (it happens to us all!) and offer a variety of options to make...

  7. What's New in CRISPR - November 2021

    Type
    Blog Post
    ...packaging of cargo sequences in the capsid, in an approach called Selective Endogenous eNcapsidation for ...therapeutically relevant gene targets and expand the application to the possible treatment of genetic disease....

  8. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    ...are particularly useful for certain research applications. Many secondary antibodies are polyclonal, as...antibody you’ll want to be sure to choose the appropriate controls to be sure the staining you are seeing...

  9. To Each HIS Own

    Type
    Blog Post
    ...Janknecht 1991, Hochuli 1988). In this purification approach, the metal ions are immobilized on a bead or resin...Bannwarth W, Döbeli H, Gentz R and Stüber D. Genetic Approach to Facilitate Purification of Recombinant Proteins...

  10. All in a Twist: dsRNA

    Type
    Blog Post
    ...RNA of cellular vs. viral origin and respond appropriately. When these sensing systems fail, diseases and...Fragile X syndrome, etc.). Hopefully now you can appreciate double stranded isn’t just for DNA. Untwisting...

  11. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    ... out large regions of DNA and has anti-viral application potential as a result. Fast facts – PAM requirement... has been proposed to be useful for the same applications (RNA knockdown, RNA editing, etc.). The main...

  12. Addgene: The First Twenty Years

    Type
    Blog Post
    ...Addgene is a totally invaluable resource. I really appreciate the work you do to make sharing reagents and ...electronic signatures to dramatically speed up MTA approvals. In 2013 we were thrilled to receive the Massachusetts...

  16. Plasmids 101: Cre-lox

    Type
    Blog Post
    ...proteins or co-factors, thus allowing for broad applications in a variety of experiments. LoxP (locus of ...more than two are present; intramolecular events happen with greater frequency than intermolecular events...

  18. Fluorescent Proteins 101: Introduction to FRET

    Type
    Blog Post
    ...within 1-10nm of each other and be oriented appropriately for energy transfer via dipole-dipole interaction..., which could monitor physical stresses or even apply forces (Gayrard and Borghi 2016) to a selected protein...

  19. Rabies and Neuronal Tracing

    Type
    Blog Post
    ...Wickersham et al., 2007).   Summary and RABV applications Use of G deleted RABV in conjunction with restricted...L, Ogawa SK, Vamanrao A, Uchida N. Whole-brain mapping of direct inputs to midbrain dopamine neurons. ...
Showing: 681 - 700 of 950 results