We narrowed to 954 results for: App;
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TypeBlog Post...packaging of cargo sequences in the capsid, in an approach called Selective Endogenous eNcapsidation for ...therapeutically relevant gene targets and expand the application to the possible treatment of genetic disease....
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Antibodies 101: Polyclonal Antibodies
TypeBlog Post...are particularly useful for certain research applications. Many secondary antibodies are polyclonal, as...antibody you’ll want to be sure to choose the appropriate controls to be sure the staining you are seeing... -
Addgene: The First Twenty Years
TypeBlog Post...Addgene is a totally invaluable resource. I really appreciate the work you do to make sharing reagents and ...electronic signatures to dramatically speed up MTA approvals. In 2013 we were thrilled to receive the Massachusetts... -
To Each HIS Own
TypeBlog Post...Janknecht 1991, Hochuli 1988). In this purification approach, the metal ions are immobilized on a bead or resin...Bannwarth W, Döbeli H, Gentz R and Stüber D. Genetic Approach to Facilitate Purification of Recombinant Proteins... -
All in a Twist: dsRNA
TypeBlog Post...RNA of cellular vs. viral origin and respond appropriately. When these sensing systems fail, diseases and...Fragile X syndrome, etc.). Hopefully now you can appreciate double stranded isn’t just for DNA. Untwisting... -
CRISPR 101: Cas9 vs. The Other Cas(s)
TypeBlog Post... out large regions of DNA and has anti-viral application potential as a result. Fast facts – PAM requirement... has been proposed to be useful for the same applications (RNA knockdown, RNA editing, etc.). The main... -
Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9
TypeBlog Post...derived from S. aureus. This means you can likely apply similar rational mutations to your Cas9 homolog ...may decrease worries about off target effects in applied and/or therapeutic settings. Will combining the... -
Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing
TypeBlog Post...can end with some sort of model (cartoons are appreciated) to visually sum up all of the conclusions. In... in images etc)? Did they perform all of the appropriate controls? Refer back to the methods section for... -
Plasmids 101: Cre-lox
TypeBlog Post...proteins or co-factors, thus allowing for broad applications in a variety of experiments. LoxP (locus of ...more than two are present; intramolecular events happen with greater frequency than intermolecular events... -
Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells
TypeBlog Post...potential to be used for other types of CRISPR applications. Let’s take a look at the key components of ...ranging from 11% to 100%, a level similar to other approaches. Nanoblades can also be used to non-invasively... -
Rabies and Neuronal Tracing
TypeBlog Post...Wickersham et al., 2007). Summary and RABV applications Use of G deleted RABV in conjunction with restricted...L, Ogawa SK, Vamanrao A, Uchida N. Whole-brain mapping of direct inputs to midbrain dopamine neurons. ... -
Educational Planarian Tools for Teaching Developmental Biology Techniques
TypeBlog Post...paste, these E. coli are transformed with the appropriate plasmid and dsRNA expression is activated through...phenotypes of RNAi knockdown treatment should be apparent after three dsRNA feedings. Some of the phenotypes... -
Fluorescent Proteins 101: Introduction to FRET
TypeBlog Post...within 1-10nm of each other and be oriented appropriately for energy transfer via dipole-dipole interaction..., which could monitor physical stresses or even apply forces (Gayrard and Borghi 2016) to a selected protein... -
Hot Plasmids - October 2020
TypeBlog Post... FP genes into C. elegans? By using a split FP approach. Split-wrmScarlet is a new split red FP that is...Hybrid for Multiplexed Editing and screening Applications (CHyMErA) Knockout Libraries express hybrid ... -
CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems
TypeBlog Post...found that at least 50% of on-target Cas9 edits happens within the first six hours. When added to human...B, Bondy-Denomy J (2020) Anti-CRISPR protein applications: natural brakes for CRISPR-Cas technologies.... -
How to Prepare for an Industry Interview
TypeBlog Post...noticed one of the biggest differences between applying for an academic position versus an industry position...interview is an opportunity to learn something and then apply it to the next interview. Perhaps an interviewer... -
Split Fluorescent Proteins for Studying Protein-Protein Interactions
TypeBlog Post...assess protein-protein interactions. One popular approach is to fuse a protein of interest to each part ... you find these plasmids useful. Good luck, and happy experimenting! References and Resources Additional... -
Progress Towards a PAM-Free CRISPR
TypeBlog Post...location of the cut and subsequent edit. In these applications, location takes priority over cutting efficiency...in double stranded cleavage and base editing applications, with high editing capacity in both contexts... -
Plasmids 101: The Wide World of Natural Plasmids
TypeBlog Post... for lightning-mediated gene transfer in soil. Applied and Environmental Microbiology, 67(8), 3440–3444...Bacteria:Their Characteristics and Potential Applications in Biotechnology. Critical Reviews in Biotechnology... -
Tips for Technical Support Calls
TypeBlog Post..., I’m confident that these tips and tricks will apply to any biology-related customer support interaction...company. This might seem like a no-brainer, but it happens more frequently than you might think. The Addgene...