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  1. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type
    Blog Post
    ...reporter gene, which replaces the targeted gene and provides a way to track successfully engineered cells. ...genotype each animal to identify the ones with the desired genotype. Inducible and conditional systems Conditional...

  2. How to Prepare for an Industry Interview

    Type
    Blog Post
    ...method seems simple, I found it hard to remember to describe all four parts of a STAR answer once the interview... because sometimes companies have relaxed dress codes for interviews. You can also ask HR for clarification...

  3. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    ...perform differently over time), compatible with the desired applications (such as immunohistochemistry (IHC...antibodies, we have developed a criterion that includes accuracy, strength, and reproducibility. We test...

  4. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    ...virus will not only ensure that your infection is designed in the best possible way, but it may also save...the transgene itself. Many researchers prefer to design primers that target the common features of viral...

  6. Illuminating Epigenetics with A FRET Based Biosensor

    Type
    Blog Post
    ... of the DNA with a high frequency of C and G nucleotides). The conversion of cytosine to 5-methylcytosine..., which binds selectively to lysine-methylated peptides. This construct is in the middle of a FRET pair...

  7. The Scientific Conference Poster Session: Tips for Success

    Type
    Blog Post
    ...success You’ve put a great deal of thought into designing your poster – now it’s time to practice your delivery...stepping into a sea of posters – where do you begin? Design a game plan Prior to attending the conference, ...

  8. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    ...plasmid recombination, but are also endA+. endA encodes a thermostable periplasmic endonuclease that can...exchange will prevent your cultures from reaching the desired density. The volume of your flask or culture tube...

  9. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    ...After this initial application, Stelzer’s group described the single-plane or selective-plane illumination...and acquire images of a sample from two opposing sides and merge the images, such as in the Ultramicroscope...

  11. Isolating Genomic Regions of Interest with the CRISPR System

    Type
    Blog Post
    ...region In 2013, the Hodaka Fujii Laboratory first described a method to purify a specific genomic region using...plasmid. Experimental use Since the initial report describing CRISPR-mediated purification of a specific genomic...

  12. DNA Purification Without a Kit

    Type
    Blog Post
    ...plasmid purification and DNA gel extraction method described in Kim and Morrison is nearly identical to that... Image: Kim and Morrison, 2009. This method provides DNA yield and quality similar to that obtained...

  13. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    ...case, a buffer exchange can be performed using a desalting column or an ultrafiltration concentrator. As ...suggests, you may also concentrate your prep to the desired concentration using these columns. If you are conjugating...

  14. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

    Type
    Blog Post
    ...improve antibody binding for some targets, they can destroy it for others. That said, organic solvents’ impact...critical to the outcome of your IF experiment and deserve careful thought and attention.   * There are other...

  15. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    ...Biology (gDNA → mRNA → protein): their roadmap includes RNA → DNA (Cooper & Temin, 1974), as one of its...is a turn of phrase — “pseudotransduction” — to describe the direct protein transfer of RT, which would...

  16. A Career in Grants: How To Become a Grant Professional

    Type
    Blog Post
    ...LLC, a grant professional development firm that includes the social media community GrantChat. She has ... from administration and fundraising to program design and evaluation. She and her team at J. Miller &...

  18. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    ...map these neuronal networks. For almost three decades, recombinant Adeno-associated virus (AAV) has been...symbol of opposition to the teaching of intelligent design in public schools and was started in 2005 in Kansas...

  19. Career Insights: Technical Support Specialist

    Type
    Blog Post
    ..., and a PhD is not always necessary. The job description and the candidate requirements (and maybe also...without being too commercial or “sales-y”. The work includes reviewing customer data sets, troubleshooting ...

  20. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    ...tumors.  This process is not quick. It can take decades from the first exposure to asbestos to the time...T-cells to more easily recognize and cause the destruction of cancer cells. Pembrolizumab has shown some...
Showing: 681 - 700 of 985 results