We narrowed to 824 results for: EED

1. Plasmids 101: Blue-white Screening

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   Blog Post

   ...plates: X-gal is light and temperature sensitive and needs to be added to media after autoclaving. If spread...

2. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

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   ...also a factor; the pol lll promoter and terminator need be encoded only once, and each <200 bp tRNA-gRNA...

3. CRISPR 101: Non-Homologous End Joining

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   ... common to biological sources of DSBs, and which need to be processed. Processing occurs prior to ligation...

4. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

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   Blog Post

   ...formally often enough) and give honest, critical feedback for improvement. Go to a science or career seminar...

5. RaPID Detection of RNA-protein Interactions

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   ...detect RNA-protein interactions. To address this need, the Khavari lab at Stanford created the RNA-protein...

6. RANbodies: Reporter Nanobody Fusions

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   ... DNA ladders with these plasmids from Penn State Need help picking the right fluorescence microscopy technique...

7. CRISPRainbow and Genome Visualization

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   Blog Post

   ... directed to the proper target sequence. They succeeded in labeling two different pairs of chromosomes...

8. Seven Tips for Using LinkedIn as a Scientist

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   ...on LinkedIn?” For anyone who is planning or might need to plan for a career outside academia (that would...

9. Plasmid Tools for Microbiome Studies

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   Blog Post

   ...diverse species. These results further highlight the need for in situ genetic tools for microbiome studies...

10. RNA Extraction Without A Kit

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    Blog Post

    ...foremost, be mindful of your extractions- if you need really clean RNA, it’s important to make sure that...

11. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

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    ... easier for you to find and use the plasmids you need. If you'd ever like to write about a recent plasmid...

12. Giving gRNAs a Facelift - Synthetic and Beyond

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    ...Genome Editing, 28 (2021). https://doi.org/10.3389/fgeed.2020.617910 Zou, R., Liu, Y., Wu, B., et al. Cas9...

13. All in a Twist: dsRNA

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    Blog Post

    ...duplexed RNA DNA:RNA hybrids Sometimes ssRNA just needs a buddy, and even DNA will suffice. This can result...

14. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

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    ...variety of useful resources and protocols! We invite feedback from all members of the research community to ...

15. BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

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    ...Arg) amino acids more frequently than any other. Indeed, nearly 20% of the single missense mutant alleles...

16. Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

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    Blog Post

    ... easier for you to find and use the plasmids you need. If you'd ever like to write about a recent plasmid...

17. Split-BioID: An Improved Method for Studying Protein-Protein Interactions

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    Blog Post

    ...Another potential limitation of Split-BioID is its speed of labeling. BioID requires 6-24 hours for tetracycline...

18. Plasmids for Endogenous Gene Tagging in Human Cells

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    Blog Post

    ... the insertion of the GFP tag precisely where we need it, in this example, at the C-terminus of the gene...

19. Hot Plasmids and Viral Preps - January 2021

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    Blog Post

    ... easier for you to find and use the plasmids you need. If you'd ever like to write about a recent plasmid...

20. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

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    Blog Post

    ... a Standard Curve To do that calculation, you’ll need to include a standard curve in your experiment. ...