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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 987 results for: IGH@;

Showing: 681 - 700 of 987 results

  1. Human Germline Editing Using CRISPR

    Type
    Blog Post
    ...zygotes at 18 hr post fertilization. They observed higher targeting efficiency than in iPSCs, but with the...scientists proceed with caution and with proper oversight from regulatory and ethics committees comprised... Check out Our New CRISPR Topic Page Read about High Fidelity CRISPR Variants Target Multiple Genes at...

  2. Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9

    Type
    Blog Post
    ...have less complementarity to the gRNA, they have a higher propensity to reform double helices and decrease...measurements to determine which portions of Cas9 might be important for nuclease specificity. They found...PMC4176945. 4. Kleinstiver, Benjamin P., et al. "High-fidelity CRISPR–Cas9 nucleases with no detectable...

  3. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

    Type
    Blog Post
    ..., TdTomato was seen only in cells with GFP, at a high activation frequency of 56-93%. In the converse ...Cre-DOG lacks the toxicity that can be seen with high levels of transcription activation domains. Both...constructing split variants should be relatively straightforward, but it is also possible to create split versions...

  4. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    ...chronic myeloid leukemia. Bhang says the work highlighted the importance of developing more potent ABL1...clonal dynamics in response to cancer therapy using high-complexity barcoding." Nature medicine 21.5 (2015...

  5. Career Coaching for Scientists: Why and Where Do I Find One?

    Type
    Blog Post
    ...launched an online career coaching program that might make her coaching more affordable for you. There...transitioning a scientist from academia to industry, you might want to consult the Cheeky Scientist. Their motto...Successful Industry Professionals”. This larger shop might appeal to some although most scientist coaches can...

  6. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type
    Blog Post
    .... In the first blog post in this series, I’ll highlight terminology you should be familiar with before...the use of long single-stranded DNA have led to a higher efficiency of homologous recombination for precise...

  7. Pathways Over Time Plasmids Engage Students in Functional Genomics Research

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    Blog Post
    ...undergraduate research questions. The idea is straightforward. Instructors first select a multi-gene pathway...O'Connor (left with Lab Director Doug Warner on the right) has taught courses in biochemistry, molecular cell...Addgene Videos to Learn about Basic Lab Techniques High School Students Explore Careers at Addgene Resources...

  8. Plasmids for Endogenous Gene Tagging in Human Cells

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    Blog Post
    ...understanding of cell biology. We hope that these high-quality, highly specific plasmids, as well as the instructions...flooding the cell with a plasmid containing regions of high homology to the gene with the cut, we trick the ...

  9. CRISPR in the Clinic

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    Blog Post
    ...host) as well as the introduction of the cancer-fighting component, CAR. Clinical trials are underway to...allele specifically. Early-stage trials showed a high success rate in clearing misfolded protein levels...Regarding effectiveness, if the editing frequency isn’t high enough to make a physiological difference, then ...

  10. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    ...ER lumen reaches the sub-millimolar range, much higher than in the cytosol. Thus, for the ER you would...subsequent change in fluorescence only occurs at high calcium concentrations. This will reduce noise and...simultaneous (1) user-controlled illumination with violet light and (2) exposure to increased intracellular calcium...

  11. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    ...recombinase systems. Picking Ligands Picking the right ligand is also important for building a chemogenetic...pharmacologically selective effector molecules (PSEMs). Higher potency means lower drug doses, which decreases... CH, Coward P, Degtyarev MY, Lee EK, Kwa AT, Hennighausen L, Bujard H, Fishman GI, Conklin BR (1999) Conditional...

  12. Tips for Technical Support Calls

    Type
    Blog Post
    ...company. This might seem like a no-brainer, but it happens more frequently than you might think. The Addgene... crucial information about a product is printed right on the tube itself! Is the answer to your question...

  13. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    ...lucky" and find a great gRNA with a proximal PAM right where you need it, the odds of this occurring are...reliability. Notably, the SpRY variant didn’t display higher off-target effects than SpCas9, a pleasant surprise...stranded cleavage and base editing applications, with high editing capacity in both contexts for NRN PAMs and...

  14. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

    Type
    Blog Post
    ...endogenous retrovirus Rless glycoprotein (BaEVRless) had high rates of transduction for most cell types they tested...existing CRISPR delivery technologies, Nanoblades had higher rates for editing efficiency and lower rates of...zygotes, which results in albinism in mice. Of the eight mice born from Tyr Nanoblade treated zygotes, five...

  15. Polymerase Chain Reaction Overview and Applications

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    Blog Post
    ...primers.  Extension- The temperature is then raised slightly to Taq polymerase’s ideal temperature (70-75oC...which can be time consuming and challenging to get right. ddPCR uses a water-oil emulsion droplet technology...those stated above. Multiplex PCR is also used in high throughput SNP genotyping, gene deletion analysis...

  16. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    ... of interest thus represents a straightforward means of exerting tight control over Cas9 nuclease activity...different genetic loci within a single cell. One might imagine transfecting cells with both the nuclease-positive...

  17. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    ...something silly like “See there, arginine 53 really is right there, at the end of helix 3…just like we said it...are useful. While a physical model of a protein might represent one aspect of the protein very well, it...while and you will wake up in the middle of the night dreaming about the conformational gymnastics that...

  18. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    ...colonies by (carefully) holding plates up to the light, where they appear as opaque dots (colonies should...the agarose onto the recipient plate at just the right temperature. If the agarose solution is too hot,...depending on the level of endogenous expression. We highly recommend the use of an electronic pipettor with...

  19. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

    Type
    Blog Post
    ... Sometimes people don’t know how to start. They might want to use our promoters and terminators, but then...coding sequence to the MoClo system. It’s quite straightforward really and we’ll be producing an instructional...chances of doing something amazing will be equally high as for those who go to work in cancer biology. ...

  20. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    ...to make the most of your time and sequence! You might also enjoy seeing how our quality control process...alignment results. The default setting is “Optimize for Highly similar sequences (megablast)”, which is very fast...nucleotide sequences, proteins often have repeated or highly homologous regions, which by default are ignored...
Showing: 681 - 700 of 987 results