We narrowed to 1,085 results for: IND

...Violet-excited GFP (vex) Rosa26-Cas9 I-CHIME Inducible knockout of a single gene pXPR_068 Violet-...immunotherapy targets but are embryonic lethal, hindering studies post embryogenesis. Using C-CHIME and ...preparations than the first generation CHIME (pXPR_053). Find X-CHIME plasmids here!   Marty LaFleur is a Postdoctoral... A. H. (2024). X-CHIME enables combinatorial, inducible, lineage-specific and sequential knockout of genes...

...posts Resources on Addgene.org Find plasmids for your research Find cancer research plasmids and resources...amplifications and fusions, missense mutations, and indels (Ng et al., 2018). From this data, they generated...The researchers used the collection to screen individual perturbations in vitro using Ba/F3 and MCF10A...

...plant, Drosophila and mammalian cells, and it may find wide use in biological research. Photoconvertible...color was observed after excitation at 488 nm, indicating that spontaneous conversion did not occur. Tobacco...protein. Nat Methods. 2009 Feb 6(2):131-3.  PubMed. Find plasmids from this publication at Addgene. Subach...31;8(9):771-7. doi: 10.1038/nmeth.1664. PubMed. Find plasmids from this publication at Addgene.   Addgene...

...out a career path? Many trainees are developing Individual Development Plans (IDP's) through Science Careers...easy to miss out on beneficial opportunities or to find oneself unsure of the next steps. Harvard PI Angela... look the same in every lab, but the principles behind this technique are beneficial for researchers at...   References 1. Yearly Planning Meetings: Individualized Development Plans Aren't Just More Paperwork...

... Below Bauer and Canver discuss the motivations behind this research.   Using CRISPR/Cas9 for targeted...deletion, the nondeleted allele is likely to bear an indel or inversion mutation [3]. In the CRISPR era, the...from the JOVE article here on Addgene's website and find more CRISPR resources.    Thank you to our guest... mediated by clustered regularly interspaced palindromic repeats (CRISPR)/Cas9 nuclease system in mammalian...

...about how I could fill this time. I was relieved to find out that the two hours were split up between several... work experience I had was in the food service industry, which followed a very different schedule. Fortunately...at Addgene. This training prepared me for the independent work on the QC team. I was responsible for helping...Addgene that means that it is possible to work the remainder of the three hours (outside of the core hours)...

...Understanding the biology behind brain development, learning, and behavior poses many challenges in neuroscience.... During transduction, the viral capsid proteins bind to the target cell’s surface proteins and this interaction... the AAV serotype used for a specific cell type. Find some transduction characteristics of select AAV ...our AAV guide Learn more about our viral service Find retrograde AAVs at Addgene ...

...future in science (see my previous blog on this).  Indeed, that was a primary message delivered by many of... tackle the issues with rules and practices of indirect costs.  The peer review and grant awards systems...annual NIH progress reports information on the Individual Development Plans (IDPs) for the graduate students...Novartis, opened with a discussion of the Novartis industry postdoctoral program.  His strongest comments ...

...recommend avoiding a High Quantity/Low Quality mindset for any applications. DNA quantity is fairly self...cellular debris look very similar. However, keep in mind that kit components (i.e. buffers, columns, or beads...columns or magnetic beads. Both approaches involve binding the plasmid DNA from your cleared lysate to a substrate...specifically designed with environmental sustainability in mind (Table 2). Table 2: Sustainability features highlighted...instruction manual will help explain the rationale behind each step and provide troubleshooting advice. In...

...the paper is published, while giving you peace of mind that your materials will only be made public when...will help us ensure that plasmids don’t get left behind in this status — a win-win situation for all. ...distribution instead of risking leaving them on hold indefinitely. Of course, the fastest way for us to release...out as planned, but you still hope somebody will find the plasmids useful, we’re happy to distribute unpublished...

...nuclease-deficient CRISPR-Cas system identifies and binds a genomic target using a gRNA, and an affiliated...was able to integrate DNA payloads of up to 15 kb. Find evoCAST plasmids here! The new horizons of evoCAST...tailored treatments to specific mutations require individualized optimizations, safety testing, and approval...approach. evoCAST is an important step toward that kind of gene treatment. The researchers also compared...

...Cellosaurus STR similarity search tool (CLASTR) to find cells lines similar to your STR profiles of interest...who knows where), now is as good a time as ever to find out the true identity of the line? Once you’re run...Contributing to Cellosaurus Generated a new cell? Or finding errors in the available information about a cell...over globe, with some surprising, and important, findings being shared. For instance, recently Cellosaurus...

...the Molecular Biology Reference Find protocols on plasmid cloning Find plasmids for your research ...temperature for a short period, and there are indications that Tris buffer is better than water in these...1874070701307010010 Additional resources on the Addgene blog Find five ways to purify DNA without a kit Read our blog...

...companies can recycle plastic-- you just have to find them (or keep reading). Corning takes back and recycles...take to action! These days, it’s important for individuals, companies, and universities to be sustainability... the Addgene blog Subscribe to the Addgene blog Find lab tips for your research Read another blog post...other scientists save resources by sharing plasmids Find plasmids at Addgene ...

...January. This involves going in front of a camera and kind of walking them through the steps of, for example... says Caitlin. Caitlin also works to meet the individual needs of schools interested in using eScience...what I did as an SME, and then 75% of the time I’m kind of the go-to person for my department.” Caitlin ...Addgene podcast with educator and science rap mastermind Tom McFadden Get tips about management for scientists...

...screening system and plans to employ the light‐inducible bacterial expression system pDawn to clone and...for their potential agricultural and medical use. Find More Optogenetic Tools Like pDawn  Making cassava...supplements Dr. Mrinalini Nair, University of Baroda, India Dr. Mrinalini Nair, Associate Professor (Right),...aims to leverage a glycine oxidase reaction to indirectly detect glyphosate via H2O2, one of the reaction...

...Addgene.org Find Fluorescent Protein Empy Backbones Visit Our Fluorescent Protein Guide Page Find Luciferase...more is often better. More transcription factor binding sites in a promoter lead to higher transcriptional...the power of SunTag for single molecule imaging, finding that plasma membrane-targeted CAAX-SunTag was 18...

...Resources on Addgene.org Start a Deposit Find Additional Hot Plasmids Find the Full List of Blue Flame Depositors...level system on our website:   A yellow flame indicates that a plasmid has been requested 20+ times   ...Blog Check Out the Top Requested Plasmid in 2015 Find Hot Plasmid Technologies Write about Your Favorite...

...with CRIPSR Resources on Addgene.org Find ACVR1 Plasmids Find Plasmids for Disease Research on OurSpecial...and awareness for rare disease and helps patients find the correct treatments, if available. As part of...commercially developed therapeutic antibody that binds Activin A and interferes with inappropriate activin-ACVR1...

... bench work. To really be happy, you may need to find a way to do, manage and lead all in one day.  How...is the ability to assess and initiate things independently. Taking initiative doesn’t mean going rogue,...listed a few of my favorites. Additionally, you can find more suggested resources on this topic and get the...Articles on Leadership, also can be purchased individually) ...