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We narrowed to 1,076 results for: IND;

Showing: 681 - 700 of 1076 results

  1. CRISPR Protocol for Genomic Deletions in Mammalian Cell Lines [Video]

    Type
    Blog Post
    ... Below Bauer and Canver discuss the motivations behind this research.   Using CRISPR/Cas9 for targeted...deletion, the nondeleted allele is likely to bear an indel or inversion mutation [3]. In the CRISPR era, the...from the JOVE article here on Addgene's website and find more CRISPR resources.    Thank you to our guest... mediated by clustered regularly interspaced palindromic repeats (CRISPR)/Cas9 nuclease system in mammalian...

  2. Three Key Considerations for Precise Neuronal Targeting Using AAV Technologies

    Type
    Blog Post
    ...Understanding the biology behind brain development, learning, and behavior poses many challenges in neuroscience.... During transduction, the viral capsid proteins bind to the target cell’s surface proteins and this interaction... the AAV serotype used for a specific cell type. Find some transduction characteristics of select AAV ...our AAV guide Learn more about our viral service Find retrograde AAVs at Addgene ...

  3. Working 9-5: an Addgene Co-op

    Type
    Blog Post
    ...about how I could fill this time. I was relieved to find out that the two hours were split up between several... work experience I had was in the food service industry, which followed a very different schedule. Fortunately...at Addgene. This training prepared me for the independent work on the QC team. I was responsible for helping...Addgene that means that it is possible to work the remainder of the three hours (outside of the core hours)...

  4. Future of Research Conference - Remarkable Opening Session

    Type
    Blog Post
    ...future in science (see my previous blog on this).  Indeed, that was a primary message delivered by many of... tackle the issues with rules and practices of indirect costs.  The peer review and grant awards systems...annual NIH progress reports information on the Individual Development Plans (IDPs) for the graduate students...Novartis, opened with a discussion of the Novartis industry postdoctoral program.  His strongest comments ...

  5. Selecting Your Plasmid Purification Kit

    Type
    Blog Post
    ...recommend avoiding a High Quantity/Low Quality mindset for any applications. DNA quantity is fairly self...cellular debris look very similar. However, keep in mind that kit components (i.e. buffers, columns, or beads...columns or magnetic beads. Both approaches involve binding the plasmid DNA from your cleared lysate to a substrate...specifically designed with environmental sustainability in mind (Table 2). Table 2: Sustainability features highlighted...instruction manual will help explain the rationale behind each step and provide troubleshooting advice. In...

  6. Addgene-Seeding Labs Plasmid Grant: Accelerating Science Globally - 5 Scientists, 4 Countries & 3 Continents!

    Type
    Blog Post
    ...screening system and plans to employ the light‐inducible bacterial expression system pDawn to clone and...for their potential agricultural and medical use. Find More Optogenetic Tools Like pDawn  Making cassava...supplements Dr. Mrinalini Nair, University of Baroda, India Dr. Mrinalini Nair, Associate Professor (Right),...aims to leverage a glycine oxidase reaction to indirectly detect glyphosate via H2O2, one of the reaction...

  7. SunTag and Fluorescent Imaging

    Type
    Blog Post
    ...Addgene.org Find Fluorescent Protein Empy Backbones Visit Our Fluorescent Protein Guide Page Find Luciferase...more is often better. More transcription factor binding sites in a promoter lead to higher transcriptional...the power of SunTag for single molecule imaging, finding that plasma membrane-targeted CAAX-SunTag was 18...

  8. The Blue Flame Award: Celebrating Addgene's Most Requested Depositors

    Type
    Blog Post
    ...Resources on Addgene.org Start a Deposit Find Additional Hot Plasmids Find the Full List of Blue Flame Depositors...level system on our website:   A yellow flame indicates that a plasmid has been requested 20+ times   ...Blog Check Out the Top Requested Plasmid in 2015 Find Hot Plasmid Technologies Write about Your Favorite...

  9. Running for Rare Disease, Running for FOP, Running for AJ

    Type
    Blog Post
    ...with CRIPSR Resources on Addgene.org Find ACVR1 Plasmids Find Plasmids for Disease Research on OurSpecial...and awareness for rare disease and helps patients find the correct treatments, if available. As part of...commercially developed therapeutic antibody that binds Activin A and interferes with inappropriate activin-ACVR1...

  10. CASTing Off for New Shores in Human Genome Editing

    Type
    Blog Post
    ...nuclease-deficient CRISPR-Cas system identifies and binds a genomic target using a gRNA, and an affiliated...was able to integrate DNA payloads of up to 15 kb. Find evoCAST plasmids here! The new horizons of evoCAST...tailored treatments to specific mutations require individualized optimizations, safety testing, and approval...approach. evoCAST is an important step toward that kind of gene treatment. The researchers also compared...

  11. Cellosaurus: A Cell Line Information Database

    Type
    Blog Post
    ...Cellosaurus STR similarity search tool (CLASTR) to find cells lines similar to your STR profiles of interest...who knows where), now is as good a time as ever to find out the true identity of the line? Once you’re run...Contributing to Cellosaurus Generated a new cell? Or finding errors in the available information about a cell...over globe, with some surprising, and important, findings being shared. For instance, recently Cellosaurus...

  12. Oh, The Places You Can Go: Careers in Science Communication - Curriculum Development

    Type
    Blog Post
    ...January. This involves going in front of a camera and kind of walking them through the steps of, for example... says Caitlin. Caitlin also works to meet the individual needs of schools interested in using eScience...what I did as an SME, and then 75% of the time I’m kind of the go-to person for my department.” Caitlin ...Addgene podcast with educator and science rap mastermind Tom McFadden Get tips about management for scientists...

  13. What's the Best Way to Elute and Store Your Plasmid DNA?

    Type
    Blog Post
    ...the Molecular Biology Reference Find protocols on plasmid cloning Find plasmids for your research ...temperature for a short period, and there are indications that Tris buffer is better than water in these...1874070701307010010 Additional resources on the Addgene blog Find five ways to purify DNA without a kit Read our blog...

  14. Bringing Sustainable Practices to the Lab: Recycling

    Type
    Blog Post
    ...companies can recycle plastic-- you just have to find them (or keep reading). Corning takes back and recycles...take to action! These days, it’s important for individuals, companies, and universities to be sustainability... the Addgene blog Subscribe to the Addgene blog Find lab tips for your research Read another blog post...other scientists save resources by sharing plasmids Find plasmids at Addgene ...

  15. Management for Scientists: Managing vs. Leading

    Type
    Blog Post
    ... bench work. To really be happy, you may need to find a way to do, manage and lead all in one day.  How...is the ability to assess and initiate things independently. Taking initiative doesn’t mean going rogue,...listed a few of my favorites. Additionally, you can find more suggested resources on this topic and get the...Articles on Leadership, also can be purchased individually) ...

  16. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    ...short-hand for Clustered Regularly Interspaced Short Palindromic Repeats, in Pubmed returned 728 articles (3/12...because the Cas9 nickase doesn't have the ability to induce double-stranded breaks like the wildtype Cas9 does...single-cell colonies, grow them up, then perform individual genotyping (using Sanger sequencing, for example...frequently requested CRISPR plasmids at Addgene Find protocols and gRNA design tools: List of CRISPR...

  17. Using Addgene's New Viral Service for Your Research

    Type
    Blog Post
    ...can always head over to the Viral Service page to find the full list of what’s available or suggest additional...beginners just starting work with virus. You can find all of our viral vector blog posts here. If you ...    www.addgene.org On the Addgene website you’ll find our updated viral vector pages as well as expanded...Addgene.org Overview of Viral Production at Addgene Find Virus Protocols Browse All Viral Vectors ...

  18. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    ...potentially rebind and nick the newly ligated DNA. Salt helps prevent topoisomerase I from rebinding, which...the "A" gets added to all PCR products. Keep in mind that Taq polymerase has an error rate of about 1...to Verify Your Plasmid Resources on Addgene.org Find More Plasmid Cloning Protocols Catch Up On Your ...

  19. Plasmid Cloning by PCR

    Type
    Blog Post
    ...Hybridization Sequence: The region of the primer that binds to the sequence to be amplified (usually 18-21bp... we want to amplify and design primers that will bind to and replicate it. The following image shows the...5'-ATGTGGCATATCTCGAAGTAC-3' for the region that binds the ORF and we will add the EcoRI restriction site... Reference Pages Deposit Your Completed Plasmids Find the Perfect Plasmid for Your Research ...

  20. The Strength of Story Telling

    Type
    Blog Post
    ... try to be as chronological as possible — if you find yourself jumping backwards in time to explain something...your lab or science or your CV has grown as well. Find your audience and fit them You don’t need to explain... paper. Build lots of versions of your story and find the one that fits your audience. Enjoy the story...Check out our blog posts on science communication Find interviews scientists in SciComm careers Check out...
Showing: 681 - 700 of 1076 results