We narrowed to 916 results for: IRE;

1. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

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   Blog Post

   ...tools work in any plant? Patron: The MoClo kit is entirely for dicots, not grasses and wheat, but we do work... Patron: I started my Twitter account recently inspired by an academic in literature who gave a talk to...

2. Which Fluorescent Protein Should I Use?

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   ...emission is green or red light. For example, T-Sapphire, LSSmOrange, and LSSmKate. Fluorescent Sensors...many FPs (particularly those derived from GFP) requires oxygen. Therefore, these FPs cannot be used in...

3. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

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   ...think of as an operating system for engineering an entire cell. Typically when people use plasmids, they ...much easier. So you could imagine engineering the entirety of a cell. That's a big long-term technical challenge...

4. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

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   ... that if an essential gene plays a role in the desired screening phenotype, it may be missed because it's...transcriptional machinery to dCas9 and its RNA directed binding site. These methods include the fusion...

5. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

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   ...modeling isn’t up your alley, feel free to skip directly to the results. To start modeling our data, we...chemistry and chemical biology.       David R. Liu is Director of the Merkin Institute and Vice-Chair of the ...

6. Negative Can Be Positive: Open AAV Data with Addgene

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   Blog Post

   ...impacted the experiments we wanted to perform. It required a great deal of time and effort to test and optimize...J. O., Outram, S., Koenig, B. A., Pereira, S., McGuire, A. L., & Lázaro-Muñoz, G. (2020). Researcher Perspectives...

7. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

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   ...availability following cross-linking fixation; they require an additional step generally referred to as Antigen...retrieval here because it’s not quite as ubiquitously required for IF as fixing and permeabilizing.  References...

8. What’s in Your Tube?: A Quick Guide to Measuring DNA by Spectrophotometry

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   Blog Post

   ... just be sure to check that your RNase has not expired (or been forgotten).    Improving read accuracy...purification protocol. Are any of your materials expired? Did you pipette carefully? Remember to discard...

9. Antibodies 101: Producing Recombinant Antibodies

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   Blog Post

   ...choose, you may not be able to elute the antibodies directly into your preferred buffer. If that’s the case...suggests, you may also concentrate your prep to the desired concentration using these columns. If you are conjugating...

10. Better Dyeing Through Chemistry & Small Molecule Fluorophores

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    ...the frontiers of fluorescence microscopy often requires more photons. For example, moving from transient...Unlike other systems, no genetically encoded tag is required as the small-molecule binding motif targets endogenous...

11. Lentiviral Vector Uses and Overview

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    Blog Post

    ...infect. Finally, the transfer plasmid encoded the desired transgene flanked by HIV-1 long terminal repeats...integrase-deficient lentiviral vectors for applications that require the large genetic capacity of a lentiviral vector...

12. CRISPR Antimicrobials

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    Blog Post

    ... PMC4466736. 5. Ando, Hiroki, Sebastien Lemire, Diana P. Pires, and Timothy K. Lu. “Engineering Modular...

13. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

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    Blog Post

    ...affinity purification, in 2009. However, iChIP requires insertion of recognition sequences of an exogenous...into the target region. However, it struck me that direct use of such engineered DNA-binding molecules even...

14. Transferable Skills Guide: External Collaborations

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    Blog Post

    ...about its limited resources. Rather than developing entire new teams in house to solve every problem (although...collaborators’ professional developments without directly contacting them. One great way to transition from...

15. Lab to Office Culture Shock

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    Blog Post

    ...process of onboarding a new employee who's coming directly from grad school. The transition has been a bit...thought they would be working in a lab for their entire career. Yet even success in academia means you’...

16. Overcoming the AAV Size Limitation for CRISPR Delivery

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    ...drawbacks limit the utility of these systems: St1Cas9 requires a very specific PAM sequence that limits the number...SaCas9, Zhang’s group used an approach called BLESS (direct in situ breaks labeling, enrichment on streptavidin...

17. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

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    ...she had a few minutes to point me in the right direction for my future work. She graciously took me for... Candace Williams, Dave Baltrus, Geof Hannigan, Irene Newton, Juliet Johnston, Kat Milligan-Myhre, Kim...

18. Twenty Years of Sharing: Expanding Accessibility

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    Blog Post

    ... with a distributor for that country. In China, direct shipments weren’t able to clear import control...., we launched the Addgene blog for topics that required a lot of nuance or detail. By the end of 2023,...

19. How to Be an Excellent Trainee

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    Blog Post

    ...someone who provides career advice and offers direction on your scientific project. Your scientific mentor...meaningful relationships in your new lab, learn the required techniques, and be ready to take on trainees one...

20. Plasmids 101: Broad Host Range Plasmids

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    Blog Post

    ...as Rep or other initiator proteins, they do not require host proteins for replication and can maintain ...pneumophila, Methylophilus methylotrophus, Myxococcus virescens, M. xanthus, Proteus rettgeri, P. mirabilis, Providencia...