We narrowed to 937 results for: Ina;
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TypeBlog Post... THE ORIGINAL NgAgo ARTICLE DISCUSSED IN THIS POST HAS BEEN RETRACTED AND FOLLOW UP STUDIES HAVE FAILED...prevent the invading genome from “recovering” its original sequence. If you’re looking to disrupt gene function...
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Plasmids 101: A Brief History of Plasmids and an Improved eBook!
TypeBlog Post...generic term for any extrachromsomal hereditary determinant”. His proposal, however, was basically ignored... in areas such as bacterial conjugation and recombination, replication and topology, and cloning and gene... -
Don’t Wait To Launch Your Health Science Startup
TypeBlog Post...critical three letters after your name (P-H-D). Finally, having spent your entire career in science, you... you see today is far different than what was originally planned, due in large part to the feedback we... -
Even more elegant: Single injection CRISPR/Cas9 in C. elegans
TypeBlog Post...Bob Goldstein’s lab at the University of North Carolina has made CRISPR in C. elegans even easier - now...elegans genome using Cas9-triggered homologous recombination. Dickinson DJ, Ward JD, Reiner DJ, Goldstein... -
Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]
TypeBlog Post...transcriptional control of multiple loci is deftly coordinated by the eukaryotic cell for the execution of many...University of Western Ontario studying plant-based recombinant pharmaceuticals. Follow him on Twitter @AdamChinFatt... -
Extracurricular Activities for a Strong Science Career Path
TypeBlog Post...graduate and postdoc training focuses on using multidisciplinary, collaborative, and translational approaches...“Never Leave an Empty Seat” at conferences and seminars, as this is a valuable time to network Attend... -
The Importance of High Titer for AAV Transductions
TypeBlog Post...References 1.Clark, K. Reed. "Recent advances in recombinant adeno-associated virus vector production." Kidney...PMID: 11841606. 2. Schultz BR, Chamberlain JS. Recombinant Adeno-associated Virus Transduction and Integration... -
"Build Your Plasmid": The Game - Play to Teach
TypeBlog Post...have 4 hours and 35 students! After a course coordination meeting with some other professors, I went home...students had to pick the best strategy and propose a final gene therapy approach for the disease to be openly... -
Multiplexed Overexpression of Regulatory Factors (MORF) collection
TypeBlog Post...clusters of individual cells along with color coded nominated reference cell types. (Adapted from Joung et ...deliver multiple TFs to each cell, allowing for combinatorial overexpression programs on cellular processes... -
Fluorescent Protein Travel Awards - Protein Variants, a Serotonin Sensor, and an Artificial Leaf Replica System
TypeBlog Post...so that it would recognize serotonin. Using a combination of computer modeling, directed evolution, site-saturation... newly developed green serotonin sensor). In preliminary studies with the lab’s collaborators, they were... -
Unique and persistent IDs for improved reproducibility: Addgene now supports RRIDs and compact identifiers for all plasmids
TypeBlog Post...another company and catalog number changes) the original identifier loses its meaning, making it difficult...click on the link it will take you directly to the original article online. It is also designed to be persistent... -
Virtual Science Conference Coming Up? Three Addgenies Share Their Experience
TypeBlog Post...presenter had four “slides” to use for their poster, eliminating the need to zoom and scroll around the poster...4MysvpzwkP — Samuel Guay (@SamGuay_) June 18, 2020 Final thoughts on virtual conferences Virtual conferences... -
Pushing the Limits of DNA Assembly
TypeBlog Post...restriction enzymes results in scarless assembly. Finally, the NEBuilder® HiFi DNA Assembly method from New...typical two oligos, is more cost effective and eliminates the annealing step usually used to create a double-stranded... -
Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors
TypeBlog Post...Previous iterations of FLiPs only used N- and C-terminal dronpa fusions. With these various designs, Xin...can then be used to determine the amount of HMO originally in the sample. The second sensor is a fusion ... -
How Dry Ice Affects Viral Vector Sample pH & How to Avoid It
TypeBlog Post...be sufficient to return the sample back to the original pH. What about putting it back in the freezer...Zolotukhin S. A simplified purification protocol for recombinant adeno-associated virus vectors. Mol Ther Methods... -
Addgene Depositors Get More Citations
TypeBlog Post...Sloan School of Management. Professor Feng Zhang’s original 2013 gene editing paper on CRISPR/Cas amassed ... Research Methods. We also pulled the big interdisciplinary journals: Nature, Science, PLOS One, and PNAS... -
Management for Scientists: Delegating is Key
TypeBlog Post...the actions of a single person but through the coordinated efforts of many individuals. Check out Joanne's... a project progresses it is easy to forgot the original plan. Write down your plans in a shared document... -
Fluorescent Proteins 101: Luciferases
TypeBlog Post...fluorophore, which emits light. No excitation illumination needed — just the luminescent substrate. This...you used? Share your tips below! This post was originally written by Jason Niehaus in June 2014 and was... -
From Lab Bench to Leadership: Transitioning into Management as a PhD
TypeBlog Post...supportive environment to build my management skills. Finally, developing emotional intelligence is vital. Learning...collaboration. The good news: It’s a journey, not a final choice Management is not a one-way path. As careers... -
Communicating Your Science With Help From ComSciCon
TypeBlog Post...writing grant proposals to be reviewed by interdisciplinary panels. And highly successful scientists ... graduate students in different regional and disciplinary communities gather at ComSciCon's local and ...