We narrowed to 1,035 results for: LAS
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TypeBlog Post...instead of three, and we are sending hundreds of plasmid requests out each day. As you may imagine, it keeps...together.I realize I may have painted a rose-colored-glasses-esque picture of how everyone is kind and collaborative...
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Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone
TypeBlog Post...Favorable conditions - Expansion of networks The last stage is Exponential Growth, named after the growth...has worked in molecular and microbiology for the last two decades as a staff scientist, but before going... -
An Inside Look into Addgene's Dev Team
TypeBlog Post... development includes everything from tracking plasmids from the moment a deposit kit is sent to a depositor...improvements, you are benefited with reassurance your plasmids will not get lost in a freezer and your viral ... -
Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor
TypeBlog Post...PHP.B permissive phenotype in mice. They took a classic genetics approach and interbred C57BL/6J and BALB...permissive and non-permissive mouse strains. PHP.B plasmids and PHP.eB viral preps are available from Addgene... -
Designing Your Chalk Talk for the Academic Job Interview
TypeBlog Post...and a room of 5-30 faculty members. The session lasts up to an hour. You will get 5-10 minutes to present...quality control of secretory proteins in the Endoplasmic Reticulum, optimization of fluorescent proteins... -
Mesothelioma - Causes, Symptoms, and Treatment
TypeBlog Post... from injury, this protein leaks out into the cytoplasm, migrates out of the cell, and binds cells that...Library Can be Used to Study Cancer Drug Resistance Plasmid Grant to Aid Cervical Cancer Screening in Ghana... -
Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design
TypeBlog Post...similar on-targeting cutting as full-length gRNAs Lastly, the effects of guide length on off-target cuts...on Addgene.org Check out this guide to pooled plasmid libraries Find all of the CRISPR Pooled Libraries... -
Antibodies 101: Reading a Flow Plot
TypeBlog Post... is something that passes through the machine’s lasers and gives off a signal read by the machine’s sensors...the Addgene blog Conventional vs. Flow Cytometry Plasmid-based Recombinant Monoclonal Antibodies Introduction... -
Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus
TypeBlog Post...HDR event, then the second HDR event can’t occur. Lastly, depending on its design, donor A may be capable...therapy Learn about the parts of an AAV transfer plasmid Resources on Addgene.org: Check out the Addgene... -
3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells
TypeBlog Post...ideally be around 5 to 20 bp away from the cut site. Lastly, if a heterozygous event is desired but only guide...Ribonucleoproteins Resources on Addgene.org Browse All CRISPR Plasmids Check Out Our CRISPR Guide Pages Find Validated... -
Fluorescent Proteins 101: When GFP lets you down
TypeBlog Post...high affinity to fluorophores. Examples are the FlAsH tag, the His tag and several others. Another peptide-based...protein fusions Browse the Fluorescent Proteins Plasmid Kits ... -
Transferable Skills Guide: Time Management
TypeBlog Post...spend accomplishing small tasks (like... preparing plasmids to send to Addgene). Following my advice from ... copied below): Read new Zhang Lab Paper Find plasmids similar to those in the paper Ask Mary G. about... -
An Integrin Antibody Toolkit from IPI
TypeBlog Post...the three αV integrins expressed on the BJ-5ta fibroblast cells, integrin αVβ1 was critical in initiating... readily accessible recombinant antibodies and plasmids. To encourage collaboration and education in protein... -
How to Negotiate a Successful Lab Start Up Budget
TypeBlog Post...and can include: equipment, reagents, animals, glassware, and even people. When developing a budget, I ...reagents and consumables (such as pipet tips, glassware, etc), get a simple list from your current P.I... -
How to Design Your gRNA for CRISPR Genome Editing
TypeBlog Post...Originally published May 3, 2017 and last updated Sep 24, 2020 This post was contributed by guest blogger...CRISPR Basics with Our CRISPR Guide Find CRISPR Plasmids for Your Research Find CRISPR Pooled Libraries... -
Viral Vectors 101: AAV Variables That Matter
TypeBlog Post...fibrillary associated protein (GFAP), or tyrosine-hydroxylase (TH), can limit which cells express your gene...three partial sequences of the human tyrosine hydroxylase promoter in vivo. Molecular Therapy - Methods... -
Antibodies 101: Multiplex Immunofluorescence
TypeBlog Post...targets. Or, it might be sufficient to describe or classify the labeled structures visually; for example, ... J., & Trimmer, J. S. (2019). A toolbox of IgG subclass-switched recombinant monoclonal antibodies for... -
Deep Dive: Statistical Tests (Comparisons)
TypeBlog Post... if they are statistically different. They are classic parametric tests and one of the simplest statistical.... 10.1097/SMJ.0b013e3182498ad5 Curran-Everett, Douglas. Explorations in statistics: statistical facets... -
Sequencing Options for CRISPR Genotyping
TypeBlog Post...PMC3773023. 2. Vouillot, Léna, Aurore Thélie, and Nicolas Pollet. "Comparison of T7E1 and surveyor mismatch...Addgene.org View Our CRISPR Guide Pages Find CRISPR Plasmids for Your Experiments Get Ready-to-Use CRISPR Lentivirus... -
Viral Vectors 101 eBook 2nd Edition
TypeBlog Post...on the Addgene blog CRISPR 101 eBook en español Plasmids 101 eBook 4th edition Resources on addgene.org...