We narrowed to 818 results for: Mal

1. Addgene’s Viral Service - Why Virus? Why Now?

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   Blog Post

   ...the middle of 2016 Addgene started distributing a small but growing catalog of ready-made AAV and Lentiviral...

2. A Novice's Perspective on Neuroscience - SfN2017

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   Blog Post

   ...delivering genes to many different cell types with minimal immunological effects. AAVs are used in experiments...

3. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

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   Blog Post

   ... genes for noninvasive imaging of microbes in mammalian hosts Article contributed by Lukas Morgan  Listen...

4. Q&A with Karen Katz and Carl Paratore, the Newest Members of the Addgene Board of Directors

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   Blog Post

   ...Businesswomen’s Association. We were involved in smaller group activities through the HBA and I got to know...

5. Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

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   Blog Post

   ...light is in an open conformation.  Nanobodies are a small (~15 kDa), single monomeric variable heavy chain...

6. Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies

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   Blog Post

   ...your docking site is located). This amounts to a small difference of two or three weeks. 3. How would you...

7. Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

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   Blog Post

   ...starvation on cancer therapy. Findings in previous animal studies suggested that fasting has a positive effect...

8. Hot Plasmids - May 2022

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   Blog Post

   ...sequences with reduced risks of unwanted mutations in mammalian cells. Long sequences traditionally have higher...

9. Plasmids 101: Blue-white Screening

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   Blog Post

   ... also false negatives: These are rare, but if a small fragment is inserted in-frame, read-through can ...

10. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

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    Blog Post

    ... could typically achieve 90-99% knockdown with minimal off-target effects. The system also enabled them...

11. Kiran Musunuru on the Newest TALEN Genome-Editing System

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    Blog Post

    ...one can introduce changes into the genomes of mammalian or other cells. TALENs are engineered proteins...

12. The time and cost required to make a plasmid

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    Blog Post

    ...to use or build on work done previously, borrow small aliquots of restriction enzymes for one-off projects...

13. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

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    Blog Post

    ...great gene therapy delivery mechanism, but their small packaging capacity of 4-5 kb limits the delivery...

14. Antibodies 101: Selecting the Right Antibody

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    Blog Post

    ...better resolution as these immune complexes are smaller than a primary:secondaries:conjugates complex. ...

15. Transferable Skills Guide: Problem Solving

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    Blog Post

    ...survey found that many researchers feel they lack formal training in a variety of transferable skills. At...

16. Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

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    Blog Post

    ...pooled library of mutant sensors expressed in a mammalian cell line engineered to be electrically excitable...

17. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

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    Blog Post

    ...quantified for individual cells, and the number of 'normal' looking cells can be counted. This assay will ...

18. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

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    Blog Post

    ...further away. While constitutive promoters drove maximal gene expression near genomic origins of replication...

19. Transferable Skills Guide: Cross-team Communication

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    Blog Post

    ...” “material,” “item,” and “format” all received formal definitions in our lexicon. We created documents...

20. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

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    Blog Post

    ...expression of ion channels, despite having an otherwise normal genomic background, can even lead to the formation...