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We narrowed to 1,010 results for: PLE

Showing: 681 - 700 of 1010 results

  1. CRISPR Cheat Sheet

    Type
    Blog Post
    ...single base changes in the DNA sequence without completely cutting the DNA. RNA Editors: CRISPR nucleases...CRISPR nucleases that can’t cut DNA (dCas9, for example) that are used to increase or decrease expression...library of gRNAs with a CRISPR nuclease target multiple genes in a cell population. The resulting mutant...

  2. Tips for Working in Industry & Success in Management: Interview with Crystal Shih

    Type
    Blog Post
    ...pharmaceutical company based in Switzerland. After completing her PhD in Chemistry and Chemical Engineering...working in industry and how delegating can empower people Chapter 3: How Crystal advanced at Novartis, and... and the importance of realizing that different people have different management needs. How Crystal developed...

  3. The Addgene Antibody Data Hub

    Type
    Blog Post
    ...gene/target, name, application, pass/fail rating, sample species, antibody species, or whether the results... by Addgene's curation team for accuracy and completeness. The information is then formatted and published...application. The submission process is easy, with simple, descriptive fields, drop-down boxes when appropriate...

  4. What's New in CRISPR - Winter 2018

    Type
    Blog Post
    ... they have developed this further to provide multiplexed detection, higher sensitivity, and more. Read...lab compared the efficiency and specificity of multiple wild-type and engineered variants of Cas nucleases...included in the next What’s New in CRISPR blog post, please let us know! Additional resources on the Addgene...

  5. A Scientist's Guide to Ebola

    Type
    Blog Post
    ...looking to delve deeper or stay tuned, there are plenty of online resources available. I recommend a few...Science Translational Medicine have made their complete collection of relevant papers freely available...you have information or another resource to add, please tell us about it in the comments. ...

  6. Download Science-Themed Zoom Backgrounds from Addgene

    Type
    Blog Post
    ... Aavery in this Zoom video background. Want the purple bacteria video background with mascots Blugene ...and more!) on Addgene’s Help Center. Download the purple bacteria background.       Are none of those ...Addgene’s Science Careers blog topic. Download the purple lab-themed background.     We hope these science...

  7. What's New in CRISPR - May 2019

    Type
    Blog Post
    ...template covalently attached to the Cas9-gRNA RNP complex via the fused HUH endonuclease PCV. This increases...Neisseria meningitidis, Nme2Cas9, that recognizes a simple dinucleotide PAM (N4CC), lending to high target...included in the next What’s New in CRISPR blog post, please let us know. We also welcome blog posts from guest...

  8. 6 Tips for Analyzing and Troubleshooting Sanger Sequencing Results

    Type
    Blog Post
    ...results Above (top right corner of this post) is an example of a seemingly clean DNA sequence (no Ns in sight...less and you might suspect contamination in your sample or consider asking your sequencing facility to ...Use a silica spin column for purification of the samples you send for DNA sequencing If your sequencing ...

  9. Q&A With Julie Cicalese, the Newest Member of the Addgene Board of Directors

    Type
    Blog Post
    ...scientific community? Access to plasmids which gives people more time to focus on their research.  How will...plan for moving forward. Sooner or later enough people will be vaccinated and we’ll have some herd immunity...raises questions like “How do you start to bring people back to on-site work?” and “How do you start to...

  10. What's New in CRISPR - Spring 2019

    Type
    Blog Post
    ...Stefan Stricker’s lab, is a single step gRNA multiplexing system that generates vectors containing high...efficiency can be controlled by manipulating complementarity between the gRNA and target. Read the paper...included in the next What’s New in CRISPR blog post, please let us know! Additional resources on the Addgene...

  11. Fluorescent Proteins 101: History of Fluorescent Proteins

    Type
    Blog Post
    ... of the coast of Washington state. Using these samples, he was able to isolate two proteins from the jellyfish...potential as a research tool. Soon, the number of people studying it grew. In particular, studies in the...organisms to find the culprits. He even obtained samples of certain ones from local pet shop aquariums in...

  12. Save Time and Money by Making Your Own Competent Cells

    Type
    Blog Post
    ... need a high efficiency of transformation (for example, if you are working with a library or an especially...making competent cells are available online. A few examples are provided here for reference: OpenWetWare ...transformation. This protocol has worked well for us for multiple E. coli strains, including DH5alpha, XL1 Blue,...

  13. Quick, Versatile Plant Transgenesis with GreenGate Plasmids

    Type
    Blog Post
    ...cassettes for selection of transgenic plants. The complete GreenGate toolkit, now available at Addgene, also...tested GreenGate in Arabidopsis, it should in principle work in any plant species. Tricks of the trade...essential for scientific progress. Also, the more people use such a system, the more building blocks will...

  14. Stepping into a Virtual Internship: My Experience at Addgene

    Type
    Blog Post
    ...interviewing fellow Addgenies. I spoke with amazing people that were a part of the different departments of...felt like I could actually make connections with people. These were some of the things that really made...by Addgene. I have met some of the most genuine people who have shared amazing advice with me. Due to ...

  15. Six Spooky Science Stories and Halloween at Addgene

    Type
    Blog Post
    ...team costumes, and spooky science stories. Yes, please! At Addgene, we love finding ways to incorporate...bird feces, which get eaten by another snail, completing its lifecycle. Halloween genes The Halloween ...and drains all the blood out. Once the blood is depleted, the tongue atrophies and falls off. The louse...

  17. SciComm with the Experts at Science in the News Part 2

    Type
    Blog Post
    ...Science Communication group started. It's as simple as getting people together at a bar. Chapter 10: Maintaining...well as a platform for scientists to be viewed as people (#ActualLivingScientists). We also discuss the ...

  18. Supporting Rare Disease Research with BeHeard 2017

    Type
    Blog Post
    ...affected by devastating diseases that affect far fewer people and therefore fail to garner much international...the Institute of Biosciences and Bioresources in Naples, Italy. His group works on spinal muscular atrophy...data from primary peritoneal mesothelioma tumor samples that they have used to identify candidate genes...

  20. The Michael Davidson Collection: One-Stop Shop for Fluorescent Proteins

    Type
    Blog Post
    ...YFP) to the new and improved fruit colors (e.g., apple, papaya, and tomato) and the photoactivatable fluors...quickly. Researchers should keep in mind that a very simple sequential or double restriction enzyme digest ...on the corresponding plasmid pages (e.g. see Supplemental Documents here). If you’re not sure where your...
Showing: 681 - 700 of 1010 results