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We narrowed to 931 results for: Por

Showing: 681 - 700 of 931 results

  3. Coomassie Purity Stain of Recombinant Antibodies

    Type
    Protocol
    ...IgG standards in Google Sheets. Open exported CSV file. Select import. Choose file. Format as needed. Select...that sharing the full details of our protocols supports reproducibility and accelerates science. Here,...information is solely for transparency intended to support reproducibility in science. Last Update: June 14...intensity of the protein bands to the overall lane. Import the gel image into ImageJ. Select File . Select... each peak by selecting the center of the peak. Export results as a csv file. In the results table, select...

  5. Great Results Start with Great Standard Curves

    Type
    Blog Post
    ...plotting the entire data set, plot only the linear portion. This allows you to use a simple linear regression... enough if your unknown lies within the linear portion of the graph. It does restrict the dynamic range...

  6. Summer SciComm Series: Modes of Communication

    Type
    Blog Post
    ...channels both digitally and physically. Easy to incorporate visuals if needed. Traditional media formats ...assuming a high resource geographic location) Opportunity for real time engagement while allowing for recording...

  7. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    ... genetic engineering of a tag or fusion with a reporter enzyme. For multicolor staining, careful consideration...al. Before picking a secondary nanobody, it’s important to review a few characteristics of your primary...

  9. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    ...gut, and other organs to the CNS, are also an important target for gene delivery, especially for the study...3293995. Additional Resources on the Addgene Blog Important considerations when using AAVs Learn more about...

  11. Plasmids 101: Colony PCR

    Type
    Blog Post
    ...colony PCR primers The first and perhaps most important step to colony PCR is designing primers. There...for cloning. This type of control is especially important for insert-specific primers. If your negative ...

  13. Plasmids 101: Protein tags

    Type
    Blog Post
    ... Protein tags are usually smallish peptides incorporated into a translated protein. As depicted in the... are available, but scientists would need to incorporate their recognition sites into their protein tag...

  14. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    ...that includes a CMV or RSV promoter as well as a portion of the 5'LTR. Including a chimeric 5'LTR removes...self-inactivating, which is achieved by deleting a large portion of the 3'LTR in the transfer plasmid. The deletion...

  15. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    ...encountering flow data in the literature. Data reported from flow cytometry experiments can be a little...correlates with the occurrence of post-transplant opportunistic infection in liver and kidney recipients. World...

  16. Viral Production at Addgene

    Type
    Blog Post
    ...expression and/or function. These data are sometimes reported or posted on the material page for the corresponding...recipient’s initial thaw will be accounted for in our reported titers. Lentiviral vectors are titered using a...

  17. Viral Vectors 101: Preparing Pooled Libraries

    Type
    Blog Post
    ...all of which need to be represented in the same proportion after amplification as they were prior to amplification...this guide, we hope you feel more confident incorporating them into your research. And, of course, if ...

  19. Transferable Skills Guide: Creativity

    Type
    Blog Post
    ...sharing their ideas? And how do we give people the opportunity to speak out and prevent groupthink? Here are...implemented, impacted the entire company. It is important to note that the person who submitted the idea...

  20. How to Write a Scientific Review Article

    Type
    Blog Post
    ... member of a class of proteins was mutated, as reported in various studies. We made a table that listed...for each protein we listed all the studies that reported mutations in that protein (including how frequently...
Showing: 681 - 700 of 931 results