We narrowed to 1,009 results for: RING

1. Our Most Popular Blog Posts from 2019

   Type

   Blog Post

   ...Farewell 2019, and hello 2020! As we're gearing up to start a new decade, we’re also looking back at ...strategies for generating GFP fusion proteins. From considering protein size and shape, linker sequences, and...

2. SciComm with the Experts at Science in the News Part 2

   Type

   Blog Post

   ...broken down below for specific topics discussed during the interview. Happy listening! Listen to the interview... Sit'N Listen - In their podcast, the SITN team brings in scientists from a variety of fields as well ...

3. Seeing Red: Simple GFP Photoconversion

   Type

   Blog Post

   ...turns out this protein has a few more secrets - during a collaboration, members of Maureen Hanson’s and... Mary Gearing...

4. Easing the Protein Purification Process with pCri

   Type

   Blog Post

   ... the RCSB Protein Data Bank and PDBsum. After acquiring this information, you can then pick an expression... Mary Gearing...

5. Science as Art

   Type

   Blog Post

   ...www.microworldsphotography.com/. Another artist who spent his life blurring the lines between science and art was Albert G...smaller competitions are now taking place and gathering momentum, including in Cambridge and Bristol. ...

6. CRISPR Protocol for Genomic Deletions in Mammalian Cell Lines [Video]

   Type

   Blog Post

   ...ectopic reporter assays. We have been interested in exploring the function of non-coding regulatory elements...Orkin and Daniel Bauer. He received his BSE in Bioengineering from the University of Pennsylvania.     References...

7. New Norepinephrine Indicators: nLightG and nLightR

   Type

   Blog Post

   ...the sensitivity of nLightG. Many thanks to Mike Waring and Tommaso Patriarchi for their assistance with.... (2023). Sensitive multicolor indicators for monitoring norepinephrine in vivo. Nature Methods, 20(9)...

8. What's New in CRISPR - March 2020

   Type

   Blog Post

   .... Read the paper in Cellular and Molecular Bioengineering Find the optogenetic repressor plasmids Cytosine...RNA-guided methylcytosine editing by TET1 by co-delivering TET1 and protein factors for linking methylcytosine...

9. Increasing Your Work's Visibility with Addgene: Citation, Search, and Collections

   Type

   Blog Post

   ... tempting to rely on the citation number when comparing two plasmids, we recommend reviewing the cited...depositors, giving us as much information as possible during the deposit process. Special collections pages ...

10. Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening

    Type

    Blog Post

    ...Eric Lander, comprise 73,171 guide RNAs (gRNAs) covering a total of 7,114 human genes. Unlike the GeCKO...cycle proteins, and more.  “In the past, genome engineering with CRISPRs was limited to modification of a...

11. Viral Vectors 101: Parts of the AAV Packaging Plasmid

    Type

    Blog Post

    ...the AAV p40 promoter. Alternative splicing and differing translational start sites generate the three VP...ratio (VP1:VP2:VP3). There are eleven naturally occurring AAV serotypes that have thus far been identified...

12. Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation

    Type

    Blog Post

    ...gene. In cells where the stop cassette is removed during the recombination step, the effector is expressed...Learn about Gateway cloning Read about genome engineering in flies Learn more about controlling gene expression...

13. Plasmids 101: What is a plasmid?

    Type

    Blog Post

    ...reporter plasmids, viral plasmids, and genome engineering plasmids. Some of the many things that plasmids...controlled changes to an organism’s genome (genome engineering) Produce synthetic viruses that can be used in...

14. An Addgene Co-op: The Intersection of Fun, Friendship, and Work

    Type

    Blog Post

    ... lot of different worries that come along with entering the workforce, but my main concerns were building...larger content and marketing team, and I started preparing for my first official professional presentation...

15. Hot Plasmids and Viral Preps - July 2021

    Type

    Blog Post

    ... organoids derived from other tissue-types. By bringing together CRISPR-based approaches and organoid ...the fluorescence intensity of the cpGFP without altering that of the internal control (Fig 1). Because ...

16. We're Updating Our Hold for Publication Policy

    Type

    Blog Post

    ...any paper at all. Posting preprints and openly sharing materials are both excellent ways to provide transparency... articles. But we do encourage you to consider sharing them openly as soon as that preprint is online!...

17. Oh, The Places You Can Go: Careers in Science Communication - Product Development

    Type

    Blog Post

    ...that I wanted to leave the bench.” Her path to figuring out her career plans was paved with lots of writing...provides scientific editing services. “I was working during the day and editing at night. It was not the easiest...

18. Bricking Science: Portraying Scientific Reality Through LEGO

    Type

    Blog Post

    ...laboratory routine. We hope that sharing and laughing at these struggles will bring people together and make ...

19. Tips for Improving Your Next Manuscript

    Type

    Blog Post

    ... online course had three aspects to it: videos starring Roberto Kolter, webinars taught by Peggy Cotter...the online class, we met as a group for sessions covering individual portions of the manuscript. Stephen...

20. The Stingy Scientist: How the Baby Gel Box Was Born

    Type

    Blog Post

    ... science project started when I realized I was pouring large agarose gels for DNA visualization or isolation... had to be careful not to stick your fingers in during a run or risk minor electrocution.  These days ...