We narrowed to 1,006 results for: RING

1. Seeing Red: Simple GFP Photoconversion

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   Blog Post

   ...turns out this protein has a few more secrets - during a collaboration, members of Maureen Hanson’s and... Mary Gearing...

2. Easing the Protein Purification Process with pCri

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   Blog Post

   ... the RCSB Protein Data Bank and PDBsum. After acquiring this information, you can then pick an expression... Mary Gearing...

3. Science as Art

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   Blog Post

   ...www.microworldsphotography.com/. Another artist who spent his life blurring the lines between science and art was Albert G...smaller competitions are now taking place and gathering momentum, including in Cambridge and Bristol. ...

4. CRISPR Protocol for Genomic Deletions in Mammalian Cell Lines [Video]

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   Blog Post

   ...ectopic reporter assays. We have been interested in exploring the function of non-coding regulatory elements...Orkin and Daniel Bauer. He received his BSE in Bioengineering from the University of Pennsylvania.     References...

5. New Norepinephrine Indicators: nLightG and nLightR

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   ...the sensitivity of nLightG. Many thanks to Mike Waring and Tommaso Patriarchi for their assistance with.... (2023). Sensitive multicolor indicators for monitoring norepinephrine in vivo. Nature Methods, 20(9)...

6. What's New in CRISPR - March 2020

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   .... Read the paper in Cellular and Molecular Bioengineering Find the optogenetic repressor plasmids Cytosine...RNA-guided methylcytosine editing by TET1 by co-delivering TET1 and protein factors for linking methylcytosine...

7. Increasing Your Work's Visibility with Addgene: Citation, Search, and Collections

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   Blog Post

   ... tempting to rely on the citation number when comparing two plasmids, we recommend reviewing the cited...depositors, giving us as much information as possible during the deposit process. Special collections pages ...

8. Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening

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   ...Eric Lander, comprise 73,171 guide RNAs (gRNAs) covering a total of 7,114 human genes. Unlike the GeCKO...cycle proteins, and more.  “In the past, genome engineering with CRISPRs was limited to modification of a...

9. An Addgene Co-op: The Intersection of Fun, Friendship, and Work

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   Blog Post

   ... lot of different worries that come along with entering the workforce, but my main concerns were building...larger content and marketing team, and I started preparing for my first official professional presentation...

10. Hot Plasmids and Viral Preps - July 2021

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    Blog Post

    ... organoids derived from other tissue-types. By bringing together CRISPR-based approaches and organoid ...the fluorescence intensity of the cpGFP without altering that of the internal control (Fig 1). Because ...

11. Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation

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    Blog Post

    ...gene. In cells where the stop cassette is removed during the recombination step, the effector is expressed...Learn about Gateway cloning Read about genome engineering in flies Learn more about controlling gene expression...

12. Plasmids 101: What is a plasmid?

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    Blog Post

    ...reporter plasmids, viral plasmids, and genome engineering plasmids. Some of the many things that plasmids...controlled changes to an organism’s genome (genome engineering) Produce synthetic viruses that can be used in...

13. Oh, The Places You Can Go: Careers in Science Communication - Product Development

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    Blog Post

    ...that I wanted to leave the bench.” Her path to figuring out her career plans was paved with lots of writing...provides scientific editing services. “I was working during the day and editing at night. It was not the easiest...

14. Bricking Science: Portraying Scientific Reality Through LEGO

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    Blog Post

    ...laboratory routine. We hope that sharing and laughing at these struggles will bring people together and make ...

15. Tips for Improving Your Next Manuscript

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    Blog Post

    ... online course had three aspects to it: videos starring Roberto Kolter, webinars taught by Peggy Cotter...the online class, we met as a group for sessions covering individual portions of the manuscript. Stephen...

16. Viral Vectors 101: Parts of the AAV Packaging Plasmid

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    ...the AAV p40 promoter. Alternative splicing and differing translational start sites generate the three VP...ratio (VP1:VP2:VP3). There are eleven naturally occurring AAV serotypes that have thus far been identified...

17. The Stingy Scientist: How the Baby Gel Box Was Born

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    Blog Post

    ... science project started when I realized I was pouring large agarose gels for DNA visualization or isolation... had to be careful not to stick your fingers in during a run or risk minor electrocution.  These days ...

18. Viral Vectors 101: Transductions

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    Blog Post

    ...transduction?  Transduction is the process of transferring foreign nucleic acid into a cell by a viral ...Expressed episomally long-term? Expressed short-term? Answering these questions will help guide you to the right...

19. Viral Vectors 101: What is a Virus (and a Viral Vector)?

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    Blog Post

    ...infection, that is. Thankfully, through the use of engineering controls — changes made to the virus that eliminate...and Man: A History of Interactions (pp. 23–40). Springer International Publishing. https://doi.org/10.1007...

20. Hot Plasmids and Viral Preps - September 2021

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    Blog Post

    ... high G+C content genes, as validated through monitoring the expression of fluorescent proteins and oxytetracycline...-nucleotide modifications to edit DNA without requiring a double-stranded break. The Xiao Wang Lab has...