We narrowed to 1,006 results for: RING

...ectopic reporter assays. We have been interested in exploring the function of non-coding regulatory elements...Orkin and Daniel Bauer. He received his BSE in Bioengineering from the University of Pennsylvania.     References...

...the sensitivity of nLightG. Many thanks to Mike Waring and Tommaso Patriarchi for their assistance with.... (2023). Sensitive multicolor indicators for monitoring norepinephrine in vivo. Nature Methods, 20(9)...

.... Read the paper in Cellular and Molecular Bioengineering Find the optogenetic repressor plasmids Cytosine...RNA-guided methylcytosine editing by TET1 by co-delivering TET1 and protein factors for linking methylcytosine...

... tempting to rely on the citation number when comparing two plasmids, we recommend reviewing the cited...depositors, giving us as much information as possible during the deposit process. Special collections pages ...

...Eric Lander, comprise 73,171 guide RNAs (gRNAs) covering a total of 7,114 human genes. Unlike the GeCKO...cycle proteins, and more.  “In the past, genome engineering with CRISPRs was limited to modification of a...

...the AAV p40 promoter. Alternative splicing and differing translational start sites generate the three VP...ratio (VP1:VP2:VP3). There are eleven naturally occurring AAV serotypes that have thus far been identified...

...any paper at all. Posting preprints and openly sharing materials are both excellent ways to provide transparency... articles. But we do encourage you to consider sharing them openly as soon as that preprint is online!...

... lot of different worries that come along with entering the workforce, but my main concerns were building...larger content and marketing team, and I started preparing for my first official professional presentation...

... organoids derived from other tissue-types. By bringing together CRISPR-based approaches and organoid ...the fluorescence intensity of the cpGFP without altering that of the internal control (Fig 1). Because ...

...gene. In cells where the stop cassette is removed during the recombination step, the effector is expressed...Learn about Gateway cloning Read about genome engineering in flies Learn more about controlling gene expression...

...reporter plasmids, viral plasmids, and genome engineering plasmids. Some of the many things that plasmids...controlled changes to an organism’s genome (genome engineering) Produce synthetic viruses that can be used in...

...that I wanted to leave the bench.” Her path to figuring out her career plans was paved with lots of writing...provides scientific editing services. “I was working during the day and editing at night. It was not the easiest...

...laboratory routine. We hope that sharing and laughing at these struggles will bring people together and make ...

... online course had three aspects to it: videos starring Roberto Kolter, webinars taught by Peggy Cotter...the online class, we met as a group for sessions covering individual portions of the manuscript. Stephen...

... science project started when I realized I was pouring large agarose gels for DNA visualization or isolation... had to be careful not to stick your fingers in during a run or risk minor electrocution.  These days ...

...transduction?  Transduction is the process of transferring foreign nucleic acid into a cell by a viral ...Expressed episomally long-term? Expressed short-term? Answering these questions will help guide you to the right...

...infection, that is. Thankfully, through the use of engineering controls — changes made to the virus that eliminate...and Man: A History of Interactions (pp. 23–40). Springer International Publishing. https://doi.org/10.1007...

... high G+C content genes, as validated through monitoring the expression of fluorescent proteins and oxytetracycline...-nucleotide modifications to edit DNA without requiring a double-stranded break. The Xiao Wang Lab has...

...intramolecular DNA recombination. This naturally occurring process is mediated by recombinases like RecA ...bacterial selection marker and origin of replication. During bacterial culture, only the plasmid with the antibiotic...

...spatially confined experiments as well as those requiring reversibility that you cannot do with AraC,” says...MA, Ernst L, Khammash M, Di Ventura B (2021) Engineering AraC to make it responsive to light instead of...