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We narrowed to 1,586 results for: RING;

Showing: 681 - 700 of 1586 results

  1. Is this the right place for me? 8 tactics for choosing a lab

    Type
    Blog Post
    ...some of these questions in the sections below. Mentoring relationships can help identify your priorities...Alumni meetings can be the most informative way of gathering data. Former lab members can give open, revealing... Know who's boss You sign on to a lab for the mentoring only to find out that the lab head is an absentee...You are not allowed to speak to lab members alone during the interview period. People seem to be hiding ...about discussing data. There’s hesitation before answering questions about lab culture. Support for diverse...ensure that you are expanding your options and preparing for both academic and non-academic career paths...evidence of industry collaboration? If you are considering a career at a small, teaching college you might...

  2. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    ...methods for physical shearing exist as well, such as sonication and needle shearing. Essentially, any method... with the lab during its recent move to the University of Georgia). She enjoys pondering how phages get...between 1 and 3 kb results in optimal likelihood of capturing a whole RBP gene without causing issues associated...following colony PCR of 10-20 colonies using primers bordering the multiple cloning site of the vector. In order...saturate each with lysis solution (~3 ml each), pouring off any excess. When your protein induction is .... Leaving the membrane overnight in the freezer during one of these steps is also a convenient stopping...through the day, spending time with her horse, and wondering what her cat is thinking. Find her on Twitter ...

  3. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

    Type
    Blog Post
    ...for many metabolic engineering and synthetic biology goals. In metabolic engineering, the expression of...interfere with the precision needed in metabolic engineering or other synthetic biology circuits. What is ... a look at how the stabilized promoters perform during these types of fluctuations. When cells are dividing... can also cause changes in plasmid copy number. During the shift from exponential to stationary phase,...promoters were able to better modulate gene expression during shifts in growth rate as compared to a constitutive...To simulate the increase in plasmid copy number during the transition to stationary phase, Segall-Shapiro...they could alter copy number of the plasmid by altering IPTG concentrations. Higher IPTG concentration...

  4. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    ...leads to incomplete reprogramming. Additionally, lingering expression or re-expression of viral transgenes...temperature sensitive Sendai virus which is removed by culturing cells at 38℃, but screening cells for the presence...considered the safest since there’s minimal risk of lingering expression of reprogramming factors. Non-replicating...the plasmid in mammalian cells and assists in tethering the vector to the cell’s chromosomes. With the... high marks for safety.   Protein Delivery: Delivering reprogramming factors as protein is another way... week for 6 weeks, and still required further culturing to generate iPSC colonies. Reprogramming efficiencies...three germ layers (ectoderm, mesoderm, endoderm). Measuring pluripotency of mouse cells typically involves...

  5. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    ...makes sense that scientists, many of whom are venturing into genome editing for the first time, have lots... the growing PubMed article list, the Genome Engineering Using CRISPR/Cas Systems has expanded out of ...wildtype or double nickase for my CRISPR genome engineering experiments? A1: When assessing which nickase...nickase type to use for your CRISPR genome engineering experiments, consider that wildtype Cas9 with optimized...amazing resources for the scientific community and sharing these resources with Addgene. Thank you, Le! This...K., & Carroll, D. (2013). Donor DNA Utilization During Gene Targeting with Zinc-Finger Nucleases. G3 Genes... L. A., & Zhang, F. (2013). Multiplex genome engineering using CRISPR/CAS systems. Science, 339(6121),...

  6. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    ... organisms which I use to study gene regulation during developmental processes, CRISPR/Cas9 allows us ...laborious handling (screening 768 F1 animals). Recovering rare editing events in a sea of unedited animals..., which makes screening extremely rapid. This stringent selection allowed me to optimize a range of editing...al., 2018). They further built on this work, discovering that melting and rapidly cooling dsDNA repair...also found that the majority of knock-ins were occurring 24 hours post-injection, making screening for ...where the 5’ ends of the PAMs are facing outwards, ensuring the PAMs remain on the chromosome after DSB formation...Toolkit and SapTrap-SEC kit for C. elegans genome engineering have also facilitated CRISPR editing in C. elegans...

  7. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    ...the Wyss Institute for Biologically Inspired Engineering. CRISPR/Cas9 technology has revolutionized the...the fields of molecular biology and bioengineering, as it has facilitated the development of a simple ...relative nuclease efficiencies as well as to more stringent targeting rules that result in a decrease in available... as a means of modulating gene expression (6). Altering the length of gRNAs targeting different sites ...Santa Cruz, where her research is focused on engineering nanoparticles as drug delivery systems. She is...develop several popular CRISPR-based tools for altering DNA sequence or regulating gene expression.   ..., Raj, et al. "Unraveling CRISPR-Cas9 genome engineering parameters via a library-on-library approach....

  8. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type
    Blog Post
    ...make genomic alterations, bringing about a revolution in genome engineering, with new techniques rapidly...and can be used for most of the current genome engineering applications of CRISPR including: generating ...delivery. You’ve decided to use RNPs, now what? Preparing the Cas9-gRNA ribonucleoprotein The first step... the Jacob Corn and Alex Schier laboratories. Delivering Cas9-gRNA ribonucleoproteins  An advantage of...applications. This technique utilizes Cas9 proteins harboring receptor ligands (C in the figure above), which...stable in human plasma than the standard spCas9 (Harrington et al. 2017). Lastly, as with any scientific ... Addgene.  Andrew's interests include genome engineering and microbiology.     References 1. Chen, Sean...

  9. DNA Purification Without a Kit

    Type
    Blog Post
    ...proteins in solution. The pH is then lowered using a renaturing solution, which causes the proteins and genomic...purification and DNA gel extraction with glass syringe filters Starting material: bacterial cultures or...binding DNA to silica columns, it’s bound to glass syringe filters. Normally DNA does not bind silica or glass... kit takes 1-1.5 hours to complete, while this syringe-based method can be completed in 20 to 30 minutes...culture you’re working with. Overall, the glass syringe filter method is not only cheaper, but also faster...gel slices Product: DNA fragments You might be wondering, “How is milk going to help me extract DNA from... 48 kb with high yields and without degradation during the extraction procedure. Additionally, purified...

  10. Hot Plasmids: Winter 2025

    Type
    Blog Post
    ...to dicot species. In addition, they generated offspring tomato plants that inherited the genomic edit ...If they do (or if they don't!), please consider sharing your experience with other researchers by contributing...

  11. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    ...the Challenges of Lentivirus Production Tips for Titering Your Lentivirus Preps More resources on Addgene.org...recombinant proteins and viral vectors. Frontiers in Bioengineering and Biotechnology, 9. https://doi.org/10.3389...

  13. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    ...expression baseline. Copy number is then determined by comparing results to a standard curve. For CNV assays, it...gives an absolute value of DNA concentration. By comparing the number of positive droplets for the gene of...

  16. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    ...their mental model of the protein. At some point during this interrogation process, they will almost invariably...recognize a PAM sequence, allow the complementary base pairing between the guide RNA and the target DNA strand...

  17. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    ...and protein turnover rates. Additionally, by engineering FP fusions associated with cellular organelles...Cas Plasmids - Protein Tagging. Beyond simply monitoring protein localization and activity, CRISPR tagging...

  18. Reflections and Looking Ahead

    Type
    Blog Post
    ...accelerating scientific discovery. Over 150,000 plasmids empowering researchers worldwide. Countless breakthroughs... to make it easier to find what you need and to bring it in line with current Web Content Accessibility...contributions to key research papers are already inspiring higher standards in antibody use (Ayoubi et al...packaged on demand in five distinct serotypes, offering you unparalleled flexibility. But this is just...characterization data for over 150,000 plasmids, empowering bioinformaticians, data scientists, and developers...collaboration formed, and every breakthrough enabled brings us closer to making an impact on humanity's greatest...this incredible journey. Together, we're not just sharing research materials — we're accelerating the future...

  19. Management for Scientists: Seeking Feedback

    Type
    Blog Post
    ... Members periodically break, go exploring outside the team, and bring information back. No team has all...contribution short and sweet. One key to this is ensuring that the group always conducts exceptionally well-managed...manager can be efficient without delegating and empowering the team to solve problems together (tactics ...important paths of information flow is the one that brings feedback back to the manager. Team members rarely...often A one-time event doesn't work to get people sharing.   Frequently ask direct questions for specific...at formal communications events or at informal gatherings, like at lunch. Many experts recommend using ...constructive feedback and suggestions. Consider offering rewards (gift card, afternoon off, etc.) Act on...

  20. Transferable Skills Guide: External Collaborations

    Type
    Blog Post
    ...transferable skills while working in the lab and by offering advice on areas where you might need some help...important in a non-research context, especially when considering small to mid-sized companies and organizations...time I’ve had a role in organizing collaborations. During my graduate studies at Karolinska Institutet I ...what was expected, and keeping track of speakers. During your graduate or postdoctoral work there are loads...you develop will lead! Read our blog post about fostering communication in remote collaborations! Stay in...Great progress! You are collaborating with your neighboring lab, you've met interesting people at conferences...twitter and maintain a connection by commenting and sharing. LinkedIn or ResearchGate allow you to send personal...
Showing: 681 - 700 of 1586 results