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We narrowed to 1,076 results for: TOR

Showing: 681 - 700 of 1076 results

  1. MXS Chaining

    Type
    Blog Post
    ... construct. This strategy can be used to build vectors with diverse functions, and thus, is used in many...construction of plasmids with many components. The creators of the MXS-chaining method have used the technique...Shetty RP, Endy D, Knight TF. Engineering BioBrick vectors from BioBrick parts. Journal of biological engineering...

  2. Plasmids 101: Colony PCR

    Type
    Blog Post
    ...colonies. The best way to do this is by using your vector with and without an insert to verify that the primers...cultures of your positive clones. If you want to store your clones longer term, just streak the colonies...PCR primers work in the first place: the backbone vector with and without an insert. These controls are ...

  3. Plasmids 101: Protein tags

    Type
    Blog Post
    ...number of chemical and peptide-based proteosome inhibitors, glutathione S-transferase (GST), which can be...purification to get your protein as pure as possible? Vectors that offer different combinations of tags are readily...Protein (GFP) Fluorescent Protein Guide Expression Vectors from the Berkeley QB3 MacroLab Eric thanks his...

  4. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

    Type
    Blog Post
    ...applications? Fujii: We generated a series of retroviral vectors expressing tagged dCas9 and guide RNA. The retroviral...joined Addgene's Advisory Board, as well as the Editorial Board of Scientific Reports and PLoS One. Additionally...especially for young researchers. It was a really nice incubator for me. In Japan, of course, it is optimized for...

  5. Tips for Writing a Good Cover Letter

    Type
    Blog Post
    ...logistical, like briefly explaining a gap in your job history or sharing relocation plans. They can be skills-based...in the possibility of joining your team as a postdoctoral researcher. Dear Hiring Manager, I am writing...your cover letter, and if possible, send it to a mentor or colleague for a second look and helpful feedback...

  6. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    ...on FRET techniques. By combining standard donor/acceptor FRET know-how with aptamers, aptabeacons (or aptatags...fluorophore protein. Years later, Samie Jaffrey’s laboratory took advantage of their deep knowledge of the...a higher melting temperature than its Spinach ancestors, and has a lower magnesium dependence. Though ...

  7. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    ...research scientists, faculty, students, private sector researchers, or pretty much anyone applying the...studies on reducing antibiotic prescriptions for respiratory tract infections. The video abstracts provide...is not hard either. Here are some resources: ImpactStory, a website that helps researchers go beyond citation...

  8. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    ...Western blot. When choosing beads, there are several factors to consider. Agarose beads are sponge-like and ...more stringent buffers may be needed. Since many factors, including salt concentration, ionic strength and...prevent this, include protease and phosphatase inhibitors in the lysis buffer, keep samples on ice, and...

  9. Plasmids 101: Codon usage bias

    Type
    Blog Post
    ...- e.g. codon choice may interrupt transcription factor binding sites) As you might imagine, it’s not ...not easy for humans to balance all of these factors on their own. Luckily, many researchers have created...Kimberly A., et al. "Selective charging of tRNA isoacceptors induced by amino‐acid starvation." EMBO reports...

  10. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    ...time-consuming, and complex to use outside of standard laboratories. In the case of the COVID-19 pandemic, qPCR ...each enzyme. In addition, Csm6, a CRISPR type-III effector nuclease, can be used in conjunction with Cas13...single-component programmable RNA-guided RNA-targeting CRISPR effector. Science 353:aaf5573 . https://doi.org/10.1126...

  11. Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

    Type
    Blog Post
    ... delivered as a pooled library via a lentiviral vector to cell lines that either stably expressed Cas9... screen did fail to identify some known ricin-regulators, but most of these genes are also essential for...hypersensitivity, or are in an enhancer, transcription factor binding site, or a coding region. Targeting these...

  12. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    ... (LshCas13a) to begin to characterize this new effector (Abudayyeh et al., 2016). In the endogenous CRISPR...fluorophore. The fluorescent signal can be used as an indicator to determine whether the target sequence is present...single-component programmable RNA-guided RNA-targeting CRISPR effector." Science 353(6299) (2016):aaf5573. PubMed PMID...

  13. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    ...CRISPR, RNAi methods are often quicker and the repositories of validated small RNAs are vast and difficult...processing Following delivery into cells using viral vectors or plasmids, shRNAs hijack the endogenous miRNA...cloning strategies for constructing shRNA expression vectors. BMC Biotechnology, 6(1). https://doi.org/10.1186...

  14. Five Popular Model Organisms

    Type
    Blog Post
    ... al., 2009). By injecting mice with retroviral vectors that harbored different mutations found in leukemia...Gerald Rubin’s lab who created a set of modular vectors that can be used to optimally control gene expression...discovery of RNAi in the worm actually rewarded depositors Andrew Fire and Craig Mello a Nobel Prize in ...

  15. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

    Type
    Blog Post
    ...either by electroporation of plasmids, or using AAV vectors. The Imai lab also developed a version of Tetbow...Livet, Jean, et al. "Transgenic strategies for combinatorial expression of fluorescent proteins in the nervous...Markus Grompe. "Clonal tracing of Sox9+ liver progenitors in mouse oval cell injury." Hepatology 60.1 (...

  16. Plasmids 101: Biotinylation

    Type
    Blog Post
    ... the microflora that reside there. Biotin is a cofactor that in its active form is covalently attached...consists of a biotin molecule attached to a photoreactive arylazide group. Under strong visible light,...single-step purification of tagged transcription factors in mammalian cells and transgenic mice.” PNAS 100.13...

  17. Transferable Skills Guide: Time Management

    Type
    Blog Post
    ...too many activities too close to one another. Monitoring task progress. For this, I always make sure I... lists. Because I know what my goals are (see monitoring task progress), and I know that I’ve blocked ... give it a final read Browse list of previous depositors from Boston University Contact Dr. Gilmore directly...

  18. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    ...introduce or remove restriction sites), in mapping of regulatory elements (to mutate promoters/enhancers in reporter... addition to the “A” site already in the parent vector). Also shown are the “B” restriction sites, which...Browse Our Molecular Biology Protocols Find Empty Vectors for Your Next Cloning Experiment ...

  19. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    ...of multiple circular DNA forms of colicinogenic factor E-1 from Proteus mirabilis. Biochemistry. 1968 ...Guttman A, Thompson L. Vaccine Plasmid Topology Monitoring by Capillary Gel Electrophoresis. Curr Mol Med... JA, Carnes AE, Hodgson CP. Plasmid DNA vaccine vector design: impact on efficacy, safety and upstream...

  20. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    ...Aydin S (2015) A short history, principles, and types of ELISA, and our laboratory experience with peptide...knockout line for validation, please view this tutorial from Stuart Orkin's and Daniel Bauer's labs.  ...
Showing: 681 - 700 of 1076 results