...compatible or not.  Using plasmid incompatibility for therapeutic advantage Although it may seem like plasmid incompatibility...

...sensitization and might open new ways for a novel therapeutic approach against drug addiction. Anxiety tests...

...capsid variants could unintentionally limit the utility of candidate capsids.  Are you interested in using...

...laundry list of molecules to screen has unclear utility. You cannot predict what will happen. Without the...

...t have to be long and it doesn’t have to be revolutionary, but you could include a few comments on where...

...and its subsequent repair. Additionally, the distribution of enrichment and depletion scores for safe-...

...minutes. Get to know your collaborators from other institutions personally on conference calls and make sure...

...PILATeS suitable for live imaging with a time resolution of minutes.  Find PILATeS plasmids here! Zhong...

... AAVs preps from 2016–2023. B) Inset showing distribution of only GCaMP6, jGCaMP7, and jGCaMP8 per year...

...interacts with DnaA, stabilizing its binding and contributing to DNA unwinding. This promotes initiation at...

...Addgene Executive director, Joanne Kamens, recently participated in a Reddit AMA (short for “Ask Me Anything...

...experiments. There are far too many behaviors that have utilized optogenetics to be fully summarized in a short...

...? As mentioned above, many expression plasmids utilize inducible promoters, which are 'inactive' until...

...considered when interpreting results.  The recent revolution in genome editing methods (most notably CRISPR...

...strand. Each degenerate primer set contains three consecutive randomized bases (NNN) at a given codon site ...

...protocol, multiplex IF can give you impactful and beautiful results. Performing an immunofluorescence experiment...

...Addgene to accelerate biomedical research by distributing antibodies developed by IPI. Our integrin antibodies...

...local temperature sensing with sub-millisecond resolution and sub-degree temperature differences under ...

...engineering world by storm owing to its ease of use and utility in a wide variety of organisms.  While much of ...

...ensure that you are in compliance with your institution’s biosafety regulations. Last Update: February...Leupeptin, Millipore Sigma L2884 Aprotinin saline solution, Millipore Sigma A6279 Before Starting Warm the...µm filter. Prepare 10 mL aliquots and store the solution at -20 °C. 1 mg/mL PEI-MAX Add 1 g of PEI-MAX ...but check for the presence of particles still in solution. Continue to stir until all particles have dissolved... to ensure that it has not drifted. Filter the solution through a 0.22 µm filter. Prepare aliquots and...at 4 °C until use. We suggest preparing fresh solutions after one month. BCD Feed 500 mL BalanCD HEK293...at 4 °C until use. We suggest preparing fresh solutions after one month. 1000X protease inhibitor cocktail...