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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 743 results for: UTY;

Showing: 681 - 700 of 743 results

  8. Transfection for Recombinant Antibodies

    Type
    Protocol
    ...ensure that you are in compliance with your institution’s biosafety regulations. Last Update: February...Leupeptin, Millipore Sigma L2884 Aprotinin saline solution, Millipore Sigma A6279 Before Starting Warm the...µm filter. Prepare 10 mL aliquots and store the solution at -20 °C. 1 mg/mL PEI-MAX Add 1 g of PEI-MAX ...but check for the presence of particles still in solution. Continue to stir until all particles have dissolved... to ensure that it has not drifted. Filter the solution through a 0.22 µm filter. Prepare aliquots and...at 4 °C until use. We suggest preparing fresh solutions after one month. BCD Feed 500 mL BalanCD HEK293...at 4 °C until use. We suggest preparing fresh solutions after one month. 1000X protease inhibitor cocktail...

  9. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...must contact your institution’s Bio-Safety office to receive permission and institution-specific instructions...sulfate for polybrene. c. Add lentiviral particle solution from step E. For a 6 cm target plate, add between...Please follow all safety guidelines from your institution and from the CDC and NIH for work in a BL2 facility...safety practice to follow, please contact your institution’s safety office. For more information, please ...extract 10g sodium chloride 15g agar Prepare LB agar solution by dissolving 40g of LB powder in 1L of distilled...Hexadimethrine Bromide (Polybrene) Prepare a 1mg/mL solution of polybrene (Sigma-Aldrich catalog #H9268) in...in 0.9% NaCl. Autoclave to sterilize. Stock solution is stable at 4°C for up to one year. The powder form...

  10. CRISPR Library Amplification

    Type
    Protocol
    ...cuvette. Pulse. Caution Electroporation involves the use of high voltages, please use caution when activating... a second and then third 1:100 dilution). Plate 100 µL of each dilution onto a prewarmed Petri dish. Incubate...adequate time to fully gel and to dry slightly. We routinely use 350 mL of LB Agar per bioassay plate. 14 mL... mix the four tubes. Perform sequential 1:100 dilutions of the cells (add 10 µL of the pool to 990 µL ... colonies can be too great to count. Ideally, dilutions would have been sufficient to enumerate single...plate, count the second plate (ie. the 1:10,000 dilution). After 12-18 hours of growth, use spreader and...suspended in cryoprotectant like Glycerol or DMSO solutions. Quantify the individual Maxipreps by Nanodrop...

  17. General Transfection

    Type
    Protocol
    ...hydrochloric acid until the solution clears. Check the pH of the solution Use hydrochloric acid or sodium...hydroxide to adjust the pH to 7.0. Typically, the solution will be basic and will need adjustment with hydrochloric...hydrochloric acid first. Pro-Tip The pH of this solution will drift pretty rapidly upon addition of acid...over or undershooting the desired pH. Allow the solution to mix for 10 min and then recheck the pH to ensure... ensure that it has not drifted. Filter the solution through a 0.22 µm membrane. Aliquot 500–1000 µL into.... Store the tubes at -80 °C. After thawing the solution can be stored at 4 °C for up to 2 months. After...

  18. Lentivirus Production

    Type
    Protocol
    ...hydrochloric acid until the solution clears. Check the pH of the solution. Use hydrochloric acid or sodium...hydroxide to adjust the pH to 7.0. Typically, the solution will be basic and will need adjustment with hydrochloric...hydrochloric acid first. Pro-Tip The pH of this solution will drift rapidly upon addition of acid or base...over or undershooting the desired pH. Allow the solution to mix for 10 min and then recheck the pH to ensure... ensure that it has not drifted. Filter the solution through a 0.22 μm membrane. Aliquot 500–1000 μL into... tubes at -20 °C or -80 °C. After thawing, the solution can be stored at 4 °C for up to 2 months. After...
Showing: 681 - 700 of 743 results