Another Pathway into Cells: iTOP
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Blog Post
...integration causing mutations and gene expression changes elsewhere in the genome. Second, expression is ...
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Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]
TypeBlog Post.... This strategy can be applied to effectively change metabolic flux. The dCas9 protein and orthogonal... -
Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping
TypeBlog Post...PubMed PMID: 31026591. Expanding the targeting range of CRISPRCas9 by engineering SpCas9 Article contributed... -
Plasmids 101: Gram Negative and Positive Bacteria
TypeBlog Post...used in, and how components of the plasmid should change based on bacteria type. The gram positive and negative... -
25 Work From Home Tips for Scientists
TypeBlog Post... Jennifer Tsang... -
Crowdfight, a Platform to Boost Scientific Collaboration During COVID-19 and Beyond
TypeBlog Post...is currently based in Spain and works on climate change. You can follow him on Twitter at @IgnacioAmigoH... -
Early Career Researcher Toolbox: Free Tools for Science Writing
TypeBlog Post...in red, words with a medium frequency of use in orange, and common words with high frequency of use in... -
Plasmids 101: NGS Quality Control for Pooled Libraries
TypeBlog Post...derived from the count_spacers.py file from Feng Zhang’s laboratory. There are a number of other analysis... -
"Build Your Plasmid": The Game - Play to Teach
TypeBlog Post...please freely share your projects with us! This exchange of ideas will help improve life science and biomedical... -
Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors
TypeBlog Post...recently presented her work at her home town in Bangladesh where it was well received. In addition to her... -
Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS
TypeBlog Post...16582903. 8. C. J. Potter, B. Tasic, E. V Russler, L. Liang, L. Luo, The Q system: a repressible binary system... -
With Our New Viral Service, We're Taking CRISPR Further!
TypeBlog Post...Institute for Biologically Inspired Engineering and the Zhang and Doench/Root labs from the Broad Institute for... -
Four Factors that Differentiate the Stem Cell Field
TypeBlog Post...field have described the Yamanaka paper as "life changing". Dr. Yamanaka himself, during his keynote address... -
Components of CRISPR/Cas9
TypeBlog Post...target binding, Cas9 undergoes a conformational change that positions the nuclease domains to cleave opposite... -
Management for Scientists: Delegating is Key
TypeBlog Post...with progress reports, meeting minutes and plan changes. Written documentation helps make sure everyone... -
Resume Writing for Non-academic Science Careers
TypeBlog Post...path to a non-academic one, one of the biggest changes (and perhaps challenges) is the need to present... -
Plasmids 101: How to Verify Your Plasmid Using a Restriction Digest Analysis
TypeBlog Post... includes a 1kb ladder (lane 1) that has bands ranging from about 500bp to 10kb, with the 3.0kb fragment... -
Pipetting Protocol
TypeProtocol...ensures the accuracy of your experiment and any changes to the amount you are dispensing can negatively...adjustment ring right below the plunger. This ring changes the pipette volume. Below that is the tip ejector...boxes that the tips come in often indicate a volume range that the tip can hold. This should give you an idea...without cross contamination as long as the tip is changed between samples. Tips can come loose in a bag, ...accurately and precisely is important since even small changes in the volume could affect your experiments. Beyond... -
Viral Vectors 101: The AAV Retrograde Serotype
TypeBlog Post... in catecholamine pathways from the spinal cord (Wang, 2018, Metcalfe, 2022) and substantia nigra (Tervo... -
Viral Vectors 101: Viruses as Biological Tools
TypeBlog Post...transduction efficiency and can effectively infect a wide range of cell types that are typically ‘difficult to transfect...