We narrowed to 954 results for: app
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TypeBlog Post...It’s at Fred Hutch.” And I hadn’t seen it. So I applied and I got it.” Science writing at Fred Hutchinson...internship similar to Susan’s NEI internship. Application information can be found here Find tips for using...
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It’s Bioelectric! An Exciting Interdisciplinary Field of Research
TypeBlog Post... for non-neural cells, ion-based communication happens at a slightly slower scale. All living cells ...After attending this meeting I had an increased appreciation for the role of ion channels and pumps in developmental... -
Troubleshooting Your Plasmid Cloning Experiment
TypeBlog Post...sequence has high GC or AT content, increase the overlapping region of the primer to 40-60 bp. It is always...these steps, you might want to reconsider your approach, and try a different cloning strategy. Once you've... -
Plasmid Tools for Microbiome Studies
TypeBlog Post...MAGIC plasmids here Paper-based synthetic biology approach to analyze gut microbiota and host biomarkers ...standards, the assay is semi-quantitative and can approximate mRNA concentration within a sample. Using this... -
Summer SciComm Series: Cognitive Load
TypeBlog Post...physicist, etc., is an immense task (it takes, ooh, approximately the length of your average PhD to achieve!) ...not communicating as clearly as you intended (it happens to us all!) and offer a variety of options to make... -
Lighting Up Cell Signaling with Photoswitchable Kinases
TypeBlog Post...spatiotemporal resolution than pharmacological or genetic approaches, but only a few such methods exist and they only...kinases good for? There are a number of potential applications for photoswitchable kinases. Below are a few... -
Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9
TypeBlog Post...derived from S. aureus. This means you can likely apply similar rational mutations to your Cas9 homolog ...may decrease worries about off target effects in applied and/or therapeutic settings. Will combining the... -
Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing
TypeBlog Post...can end with some sort of model (cartoons are appreciated) to visually sum up all of the conclusions. In... in images etc)? Did they perform all of the appropriate controls? Refer back to the methods section for... -
Plasmids 101: Cre-lox
TypeBlog Post...proteins or co-factors, thus allowing for broad applications in a variety of experiments. LoxP (locus of ...more than two are present; intramolecular events happen with greater frequency than intermolecular events... -
Plasmids 101: The Wide World of Natural Plasmids
TypeBlog Post... for lightning-mediated gene transfer in soil. Applied and Environmental Microbiology, 67(8), 3440–3444...Bacteria:Their Characteristics and Potential Applications in Biotechnology. Critical Reviews in Biotechnology... -
Progress Towards a PAM-Free CRISPR
TypeBlog Post...location of the cut and subsequent edit. In these applications, location takes priority over cutting efficiency...in double stranded cleavage and base editing applications, with high editing capacity in both contexts... -
How to Prepare for an Industry Interview
TypeBlog Post...noticed one of the biggest differences between applying for an academic position versus an industry position...interview is an opportunity to learn something and then apply it to the next interview. Perhaps an interviewer... -
Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells
TypeBlog Post...potential to be used for other types of CRISPR applications. Let’s take a look at the key components of ...ranging from 11% to 100%, a level similar to other approaches. Nanoblades can also be used to non-invasively... -
Hot Plasmids - October 2020
TypeBlog Post... FP genes into C. elegans? By using a split FP approach. Split-wrmScarlet is a new split red FP that is...Hybrid for Multiplexed Editing and screening Applications (CHyMErA) Knockout Libraries express hybrid ... -
CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems
TypeBlog Post...found that at least 50% of on-target Cas9 edits happens within the first six hours. When added to human...B, Bondy-Denomy J (2020) Anti-CRISPR protein applications: natural brakes for CRISPR-Cas technologies.... -
Rabies and Neuronal Tracing
TypeBlog Post...Wickersham et al., 2007). Summary and RABV applications Use of G deleted RABV in conjunction with restricted...L, Ogawa SK, Vamanrao A, Uchida N. Whole-brain mapping of direct inputs to midbrain dopamine neurons. ... -
Educational Planarian Tools for Teaching Developmental Biology Techniques
TypeBlog Post...paste, these E. coli are transformed with the appropriate plasmid and dsRNA expression is activated through...phenotypes of RNAi knockdown treatment should be apparent after three dsRNA feedings. Some of the phenotypes... -
Split Fluorescent Proteins for Studying Protein-Protein Interactions
TypeBlog Post...assess protein-protein interactions. One popular approach is to fuse a protein of interest to each part ... you find these plasmids useful. Good luck, and happy experimenting! References and Resources Additional... -
Fluorescent Proteins 101: Introduction to FRET
TypeBlog Post...within 1-10nm of each other and be oriented appropriately for energy transfer via dipole-dipole interaction..., which could monitor physical stresses or even apply forces (Gayrard and Borghi 2016) to a selected protein... -
Tips for Technical Support Calls
TypeBlog Post..., I’m confident that these tips and tricks will apply to any biology-related customer support interaction...company. This might seem like a no-brainer, but it happens more frequently than you might think. The Addgene...