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Showing: 681 - 700 of 954 results
  1. Troubleshooting Your Plasmid Cloning Experiment

    Type
    Blog Post
    ...sequence has high GC or AT content, increase the overlapping region of the primer to 40-60 bp. It is always...these steps, you might want to reconsider your approach, and try a different cloning strategy. Once you've...
  2. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    ...MAGIC plasmids here Paper-based synthetic biology approach to analyze gut microbiota and host biomarkers ...standards, the assay is semi-quantitative and can approximate mRNA concentration within a sample. Using this...
  3. Summer SciComm Series: Cognitive Load

    Type
    Blog Post
    ...physicist, etc., is an immense task (it takes, ooh, approximately the length of your average PhD to achieve!) ...not communicating as clearly as you intended (it happens to us all!) and offer a variety of options to make...
  4. Lighting Up Cell Signaling with Photoswitchable Kinases

    Type
    Blog Post
    ...spatiotemporal resolution than pharmacological or genetic approaches, but only a few such methods exist and they only...kinases good for? There are a number of potential applications for photoswitchable kinases.  Below are a few...
  5. Plasmids 101: Cre-lox

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    Blog Post
    ...proteins or co-factors, thus allowing for broad applications in a variety of experiments. LoxP (locus of ...more than two are present; intramolecular events happen with greater frequency than intermolecular events...
  6. Plasmids 101: The Wide World of Natural Plasmids

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    Blog Post
    ... for lightning-mediated gene transfer in soil. Applied and Environmental Microbiology, 67(8), 3440–3444...Bacteria:Their Characteristics and Potential Applications in Biotechnology. Critical Reviews in Biotechnology...
  7. Progress Towards a PAM-Free CRISPR

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    Blog Post
    ...location of the cut and subsequent edit. In these applications, location takes priority over cutting efficiency...in double stranded cleavage and base editing applications, with high editing capacity in both contexts...
  8. How to Prepare for an Industry Interview

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    Blog Post
    ...noticed one of the biggest differences between applying for an academic position versus an industry position...interview is an opportunity to learn something and then apply it to the next interview. Perhaps an interviewer...
  9. Hot Plasmids - October 2020

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    Blog Post
    ... FP genes into C. elegans? By using a split FP approach. Split-wrmScarlet is a new split red FP that is...Hybrid for Multiplexed Editing and screening Applications (CHyMErA) Knockout Libraries express hybrid ...
  10. Rabies and Neuronal Tracing

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    Blog Post
    ...Wickersham et al., 2007).   Summary and RABV applications Use of G deleted RABV in conjunction with restricted...L, Ogawa SK, Vamanrao A, Uchida N. Whole-brain mapping of direct inputs to midbrain dopamine neurons. ...
  11. Fluorescent Proteins 101: Introduction to FRET

    Type
    Blog Post
    ...within 1-10nm of each other and be oriented appropriately for energy transfer via dipole-dipole interaction..., which could monitor physical stresses or even apply forces (Gayrard and Borghi 2016) to a selected protein...
  12. Tips for Technical Support Calls

    Type
    Blog Post
    ..., I’m confident that these tips and tricks will apply to any biology-related customer support interaction...company. This might seem like a no-brainer, but it happens more frequently than you might think. The Addgene...
Showing: 681 - 700 of 954 results