We narrowed to 872 results for: cel

...multiple plasmids in one cell, it might be that you can't stably maintain them in the cell. This blog post features...

...spatio-temporal control of gene expression within a cell. Thanks to the work of Huimin Zhao and colleagues...on a version that she hopes will change the intracellular localization of RNA. “You can think of changing...

...Twenty years of accelerating scientific discovery. Over 150,000 plasmids empowering researchers worldwide... not just sharing research materials — we're accelerating the future of science. Want to learn more about...

... brain regions. You can partially control which cells are successfully infected by an AAV by altering ...control where your gene of interest is expressed.  A cell-type specific Cre can provide you with that control...

...mixture of two base editor enzymes in mammalian cells. Read the paper in bioRxiv Find Target-ACE   CRISPR...bionanoparticle Transient expression of CRISPR/Cas9 in cells has advantages like reduced off-target activity ...

...pathways that are frequently implicated in cancer cell proliferation, survival, differentiation, and apoptosis...pathway. “The idea is that if you introduce them into cells one at a time, you could identify those that confer...

...down to one, key concept: Creating a culture of excellent, effective communication between all members of...meetings with team members (don’t become known for cancelling these); ask for feedback and input on multiple...

...Last week, the Addgene 10-year Anniversary Gala celebrated all of the people who have helped Addgene grow...the Addgenies had no intention of ending the celebration. The party later moved to State Park, where I...

...Apparently, a good Jedi does not necessarily an excellent mentor make. Are your current mentors/advisors...What Kind of Mentor Do I Want To Be?” In this excellent essay Acclimatrix, an assistant professor at a...

...organizations to share materials more broadly to accelerate COVID-19 research: Sinai Health System                                     ...SARS-CoV-2 proteins in bacteria, yeast, and mammalian cells. We teamed up with Ginkgo Bioworks to quickly bring...

...small Cas protein, is active in E. coli and human cells Two years ago, Jennifer Doudna’s lab discovered ...demonstrating that CasX is active in both E. coli and human cells. Using the structure of CasX at eight different ...

...outdoors in nature or visiting museums can be excellent methods to detach from the constant stream of ...to pinpoint areas of my career and life where I excel (and thus feel grateful) and identify areas in need...

... repository of CRISPR screens Cellosaurus: A knowledge resource for cell lines IBEX Knowledge Base: A ...

... remember what happened when drug X was added to cell line Y, or the phenotype of a mouse with a specific...comparison of the same experiment from different cell lines/model systems ReFigure allows you to make...

...reading Toxic Academic Mentors by @drmellivora, an excellent blog post about workplace bullying featured on...the first place. The good news There are many excellent mentors out there. With some good pre-work you...

...about synthetic promoter AAVs for cell-type expression in retinal cells Resources on Addgene.org Find ...

.... This tag is not suitable for use in apoptotic cells as Caspase3/7 both cleave after the DVPD sequence...fluorescent marker. The GFP tag is ideal for live cell imaging, FRET, flow cytometry, or immunofluorescence...

...these issues by creating immortal antibody-producing cell lines (hybridomas) that secrete antibodies in culture...reproducible production. However, even clonal hybridoma cell lines can produce more than one mAb (Bradbury et...

...successfully used this plasmid to biotinylate the extracellular domain of the erythropoietin receptor and subsequently... Eye Institute and is particularly interested in cell signaling.     Additional Resources on the Addgene...

... life that remained unexplored. Using new single-cell sequencing approaches, the lab was able to eliminate...sample was separated into smaller samples of 5-10 cells each for sequencing, allowing for lower complexity...