We narrowed to 951 results for: cha
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TypeBlog Post...which are more likely to carry the full plasmid. Chakiath & Esposito (2018) noticed that in Stbl3 bacterial.... https://doi.org/10.1007/s13205-012-0070-8 Chakiath CS, Esposito D (2007) Improved recombinational...
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Viral Vectors 101: Transductions
TypeBlog Post...involve DNA incorporation into the cell, but the mechanisms vary. Why transduce? So why use a virus to deliver...transduction Transductions utilize the ‘normal’ mechanism of viral infiltration into a cell. The twist is... -
New Viral Vectors - Winter 2025
TypeBlog Post...Optogenetics Gordon Fishell New viral prep pAAV_BiCHATe27_dTomato_nlsdTomato AAV-PHPeB Control Gordon Fishell New... New viral prep pAAV_BiCHATe27_ChR2_mCherry AAV-PHPeB Optogenetics Gordon Fishell New viral prep pAAV_BiVIPe4... -
Celebrate Open Data Day with Addgene’s AAV Data Hub!
TypeBlog Post...our growing group of Addgene AAV data sharing champions? If you’ve recently used Addgene AAV tools, we...caption. For microscopy images, please include channel imaged, a color legend for probes/dyes/labels, ... -
10 Great Guest Posts We're Thankful For
TypeBlog Post...and retract over many microns in response to pH changes without the need for ATP or other chemical fuel... of your target organism. In this post, Chris Richardson, a postdoctoral researcher in Jacob Corn’s lab... -
Learning Technical Skills at a Lab Central Workshop
TypeBlog Post...was very contemporary and futuristic with block chairs in the hallway for people to take a break and relax...our own space. Workshops such as this give us a chance to learn something new without spending a lot of... -
Visualizing Translation at the Single Molecule Level
TypeBlog Post...TRICK reporter mRNA this displacement leads to a change in the fluorescent signal: untranslated RNAs appear...experiments, it’s important to verify that these changes do not impact translation rate, as the authors ... -
Science Career Options
TypeBlog Post...scientifically minded! Listen to Biotech Founder Michael Koeris' Journey from Grad Student to Entrepreneur...while in Grad School Listen to Biotech Founder Michael Koeris' Journey from Grad Student to Entrepreneur... -
With Our New Viral Service, We're Taking CRISPR Further!
TypeBlog Post...and proceed to use it for experiments.” - Alex Chavez, researcher at the Wyss Institute for Biologically...gotten these services up and running. Both Alex Chavez and John Doench are members of the Addgene Advisory... -
Improving Transduction Efficiency with Exosome AAVs
TypeBlog Post...production phase. However, this has not been well characterized as it is not a common event. What are exosome...2014), but scientists have yet to decipher the mechanistic aspects behind these improvements. What have... -
Plasmids 101: Gram Negative and Positive Bacteria
TypeBlog Post...+ and Chloride (Cl-) ions. When the negatively charged iodine is added, it interacts with the CV+, creating...used in, and how components of the plasmid should change based on bacteria type. The gram positive and negative... -
Trading Spaces - Visiting Scientist Edition
TypeBlog Post...scientist stint may be ideal for you. Here, we will chat about who might want to explore this role, how to...want to consider for future work placements. The chance to learn and collaborate all while experiencing... -
The MTA at Addgene
TypeBlog Post... via an online process, though the process has changed a lot over the last twenty years! This has helped...UBMTA link for the recipient organization upon purchase. We recognize exceptionally fast tech transfer... -
Networking Follow-up: I Met a Person. Now What?
TypeBlog Post...side note, my guess is that people will not be exchanging business cards for awhile or ever so your LinkedIn.... If you really got along well, suggest a coffee chat in the near future. Even just one more meeting (... -
Cultivating Community Science at BosLab
TypeBlog Post...Dave is a chemical biologist with a Ph.D. from UNC-Chapel Hill and has extensive experience targeting RNA...extract DNA, sequence it, and analyze the data to characterize the microbiomes of the different samples. The... -
Four Factors that Differentiate the Stem Cell Field
TypeBlog Post...iPSCs Symposium earlier this year, I jumped at the chance. Here was an opportunity to see Shinya Yamanaka...field have described the Yamanaka paper as "life changing". Dr. Yamanaka himself, during his keynote address... -
5 Great Apps for Lab Life
TypeBlog Post...with the content we store in them: music, emails, chats, pictures, and apps. Throughout my time as a graduate...to write your colleagues’ names in a polypeptide chain, except if their names have a B, J, X, or Z. Some... -
How to Lead a Great Meeting
TypeBlog Post...this into the agenda ensures that everyone gets a chance to talk and less vocal team members still have ...meeting a day in advance. This gives everyone a chance to review the agenda and add anything that is missing... -
Components of CRISPR/Cas9
TypeBlog Post...target binding, Cas9 undergoes a conformational change that positions the nuclease domains to cleave opposite...them in your experiments. Use the following flow chart to get you thinking about the types of experiments... -
Synthetic Photobiology: Optogenetics for E. coli
TypeBlog Post...spatially limited studies and can fluctuate with changing culture conditions. The use of low intensity light...References Refactoring and optimization of light-switchable Escherichia coli two-component systems. Schmidl...