We narrowed to 956 results for: cha
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TypeBlog Post...our growing group of Addgene AAV data sharing champions? If you’ve recently used Addgene AAV tools, we...caption. For microscopy images, please include channel imaged, a color legend for probes/dyes/labels, ...
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Scientific Peer-review: Providing Critical and Kind Feedback and Advocating for Open Science
TypeBlog Post...general comments and adjust them if my opinion changed after this more detailed look. Encourage... Tester, where she focuses on (1) salt-induced changes in root-to-shoot ratio in Arabidopsis, (2) expression... -
New Viral Vectors - Winter 2025
TypeBlog Post...Optogenetics Gordon Fishell New viral prep pAAV_BiCHATe27_dTomato_nlsdTomato AAV-PHPeB Control Gordon Fishell New... New viral prep pAAV_BiCHATe27_ChR2_mCherry AAV-PHPeB Optogenetics Gordon Fishell New viral prep pAAV_BiVIPe4... -
Hot Plasmids June 2018 - Reverse transcriptase, nanobody, and protein-DNA interaction tools
TypeBlog Post...antibody fragments typically engineered from heavy-chain only antibodies found in camelids. They possess ...H2O2, APEX2 labels electron-rich amino acid side chains (e.g. Tyrosine) with biotin. These biotin-labeled... -
Arabidopsis in Education: How the Arabidopsis Biological Resource Center Brings Plant Science to Life
TypeBlog Post...more. Downloadable materials are available free-of-charge from the ABRC Outreach website. Seeds, which are...system comes with fewer ethical and logistical challenges than does working with animals. Because Arabidopsis... -
The Strength of Story Telling
TypeBlog Post...anecdotal forms of information — identifying with a character, building emotional investment and following cause-and-effect... to date, invite people to ask questions, be approachable. Engage with your audience and try to learn ... -
10 Great Guest Posts We're Thankful For
TypeBlog Post...and retract over many microns in response to pH changes without the need for ATP or other chemical fuel... of your target organism. In this post, Chris Richardson, a postdoctoral researcher in Jacob Corn’s lab... -
Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools
TypeBlog Post...LOV2 and Zdk1 to dissociate due to conformational changes, and as a consequence, EB1 protein function is ...expansion of the genome editing toolkit. However, a challenge in optimizing base editing technologies across... -
Viral Vectors 101: Transductions
TypeBlog Post...involve DNA incorporation into the cell, but the mechanisms vary. Why transduce? So why use a virus to deliver...transduction Transductions utilize the ‘normal’ mechanism of viral infiltration into a cell. The twist is... -
HA Frankenbody, a New Imaging Tool to Visualize Single Molecules and Nascent Peptides
TypeBlog Post...antigen-binding fragments (Fabs) (Porter 1959) and single-chain variable fragments (scFvs) (Inbar et al., 1972) ...within the variable portions of heavy and light chains. Proceedings of the National Academy of Sciences... -
Plasmids 101: Gram Negative and Positive Bacteria
TypeBlog Post...+ and Chloride (Cl-) ions. When the negatively charged iodine is added, it interacts with the CV+, creating...used in, and how components of the plasmid should change based on bacteria type. The gram positive and negative... -
Improving Transduction Efficiency with Exosome AAVs
TypeBlog Post...production phase. However, this has not been well characterized as it is not a common event. What are exosome...2014), but scientists have yet to decipher the mechanistic aspects behind these improvements. What have... -
The MTA at Addgene
TypeBlog Post... via an online process, though the process has changed a lot over the last twenty years! This has helped...UBMTA link for the recipient organization upon purchase. We recognize exceptionally fast tech transfer... -
Learning Technical Skills at a Lab Central Workshop
TypeBlog Post...was very contemporary and futuristic with block chairs in the hallway for people to take a break and relax...our own space. Workshops such as this give us a chance to learn something new without spending a lot of... -
Cultivating Community Science at BosLab
TypeBlog Post...Dave is a chemical biologist with a Ph.D. from UNC-Chapel Hill and has extensive experience targeting RNA...extract DNA, sequence it, and analyze the data to characterize the microbiomes of the different samples. The... -
Science Career Options
TypeBlog Post...scientifically minded! Listen to Biotech Founder Michael Koeris' Journey from Grad Student to Entrepreneur...while in Grad School Listen to Biotech Founder Michael Koeris' Journey from Grad Student to Entrepreneur... -
With Our New Viral Service, We're Taking CRISPR Further!
TypeBlog Post...and proceed to use it for experiments.” - Alex Chavez, researcher at the Wyss Institute for Biologically...gotten these services up and running. Both Alex Chavez and John Doench are members of the Addgene Advisory... -
Trading Spaces - Visiting Scientist Edition
TypeBlog Post...scientist stint may be ideal for you. Here, we will chat about who might want to explore this role, how to...want to consider for future work placements. The chance to learn and collaborate all while experiencing... -
Four Factors that Differentiate the Stem Cell Field
TypeBlog Post...iPSCs Symposium earlier this year, I jumped at the chance. Here was an opportunity to see Shinya Yamanaka...field have described the Yamanaka paper as "life changing". Dr. Yamanaka himself, during his keynote address... -
5 Great Apps for Lab Life
TypeBlog Post...with the content we store in them: music, emails, chats, pictures, and apps. Throughout my time as a graduate...to write your colleagues’ names in a polypeptide chain, except if their names have a B, J, X, or Z. Some...