We narrowed to 1,027 results for: ell

...immunocytochemistry (ICC) when the sample is cultured cells or immunohistochemistry (IHC) with tissues), where...know if they co-localize at the same place in the cell? Or you know that the presence of several biomarkers...tissue sections, methanol- or paraformaldehyde-fixed cell samples, or other preparations. Sequential IF For...anti-CASPR (green), and anti-Kv2.1 (cyan). (B) Cerebellum labeled with anti-GABA-AR β1 (white) and anti-GABA-AR...hippocampus, or counting the number of puncta in each cell. When making figures for a paper or presentation...approach for deep phenotyping and spatial analysis of cells in complex tissues. Proceedings of the National ...

...transferable skills while you are also learning to be an excellent scientist. Leadership and management Take a lead...always involved. Demonstrating your ability to work well with others can be done in many ways… and many labs...supportive editors so your first drafts are always excellent Technical knowledge and creativity Become a “...Employers and academic departments want to be able to tell that you know about something and are passionate...local networking and trade organizations Another excellent perspective on this topic can be found on the ...

... vectors and genetically target them to specific cell populations. They demonstrated use of the LITEs ...non-invasively, its use is not restricted to cultured cells but extends to freely moving animals, as established...novel mode of optogenetic control of endogenous cellular processes." The LITE system can now be applied...no means limited to particular model organisms or cell types. “This tool is really flexible and modular...supernatant. (The details can be found here.) So tell us, readers, how will you get creative with LITE...

...plasmids to upregulate their expression in specific cells. With a more concrete understanding of the molecular...molecules targeting SMN1 and its partners in mouse cell lines. Dr. Di Schiavi hopes that these studies will... Mesothelioma  Noushin Nabavi is a Postdoctoral Fellow at the Vancouver Prostate Center and is affiliated...that affects the lining of the lungs is relatively well known, there are a variety of lesser known mesothelioma... of ~30 patient derived peritoneal mesothelioma cells lines and assess their tumorigenesis. These experiments...

...families of cell surface and secreted proteins (glypicans, integrins, and more coming soon) as well as epitope...high-throughput screening techniques such as ELISA and cell surface display are common. ELISA enables quantitative...for comparing relative affinities across mutants. Cell surface display systems, such as bacterial, yeast...presentation of your mutant libraries on the surface of cells. This enables direct screening via flow cytometry...therapies and improve their delivery methods to various cells. The wealth of smaller engineered antibody fragments...

...statistical tests – when to use them, what they can tell you, and how to identify which one is right for ..., and mode give you a summary snapshot without telling you anything about outliers or data spread within...Distribution - spread of data; typically, normal (bell curve) or skewed (see Fig. 2). Assumptions – ... up to two independent variables. This test will tell you if there are significant differences between... the means of your groups. However, it will only tell you if differences exist somewhere within your data...crop production (though you would not be able to tell if the dependent variables worked synergistically...

...circuits are generally linked to dopamine (DA), a well-known reward chemical and neurotransmitter. But ...firing patterns (or the frequency of its release) are well understood, the direct effect of DA binding to a...the brain has been in vivo microdialysis of extracellular fluid (Darvesh et al., 2011). This technique... across the semipermeable membrane from the extracellular fluid into the perfused liquid. The probe then...detection of dopamine in flies, fish, and mice." Cell 174.2 (2018): 481-496.PubMed PMID: 30007419. PubMed...

...two-photon microscopy. Cell, 18, 3408 - 3425.e29. (2022). https://doi.org/10.1016/j.cell.2022.07.013.   ...voltages. This includes monitoring of fast bursts, as well as slow up/down voltage states in deep tissue, in...voltage fluctuations. Image credit: Liu Z, et al. Cell. 2022.  Find JEDI-2p viral preps here (and here...human c-myc proto-oncogene product. Molecular and Cellular Biology, 5(12), 3610–3616. doi: https://doi.org...

...profit motive. We build this trust by keeping excellent records and maintaining confidentiality where ... Addgenies like the nonprofit company culture as well. We work every day to do a better job of enabling...Jessica, Joel, Jonathan, Justine, Karen, Kate, Kayla, Kelly, Kory, Kris, Leila, Lianna, Luke, Marcy, Mary, Max...Melina, Meron, Michael Lo., Michael Le., Michel, Michelle, Morgan, Natashia, Nicole, Olivia, Pamela, Racheal...Addgene Blog Learn about Our Collaboration with Fellow Nonprofit Seeding Labs Addgene Supports Rare Disease...

...the fall colors are inspiring you to paint your cells in equally vivid colors, you'll want to check out...AAV9-X1.1 enables expression in brain endothelial cells via an injection into the systemic circulation. ...levels compared to AAV9. You can now target these cells with AAV-CAG-GFP packaged in AAV9-X1.1!  We also...recombinase or a fluorescent marker in the various cell types of the basal ganglia. They work via newly-...before. We have a bunch of other new AAV vectors as well — including next-generation biosensors for GABA,...

... remember what happened when drug X was added to cell line Y, or the phenotype of a mouse with a specific...comparison of the same experiment from different cell lines/model systems ReFigure allows you to make...the right experiment! The ReFigure extension also tells readers if a paper they’re reading has been linked... to show that a previously used reagent performs well, or you might publish a replication experiment that...immuno-oncology. Funding agencies such as the NIH and Wellcome Trust now accept interim research products (pre-registration...

...sweat, and tears, as well as the whole point of the paper. They should be able to tell your story for you...I am writing isn’t well written, I must write everyday in order to get better. Well what does that mean...interaction of critiquing others’ manuscripts as well as being critiqued added an extra level of application...

...to learn much more about cellular DNA repair pathways. An emphasis on cellular repair post-CRISPR cleavage... a common thread at the meeting. The new Somatic Cell Genome Editing program, run through the NIH Common..., which alter genomic DNA without the need for cellular HDR.   Having had time to digest my notes, since...Improve HDR Efficiency for CRISPR Editing in Human Cells Hands-on tips for repair template design from guest...

...these issues by creating immortal antibody-producing cell lines (hybridomas) that secrete antibodies in culture...reproducible production. However, even clonal hybridoma cell lines can produce more than one mAb (Bradbury et...about recombinant antibodies in this blog post. Hello, NABOR! Addgene will bring the same open-access ...resource of plasmids encoding recombinant antibodies as well as other affinity reagents such as single-chain ...need for an open-access, molecularly defined, and well validated collection. I’m thrilled to be working...

...R-factors could be transferred between bacteria via cell-to-cell contact; however, scientists noted that, unlike...cytoplasmic factors that could be transferred between cells. At the time, these extranuclear agents of heredity...PMCID: PMC3785787. References: Lederberg, Joshua. "Cell Genetics and Hereditary Symbiosis" Physiological...Transmissible Drug Resistance in an Epidemic Strain of Salmonella Typhimurium.” The Journal of Hygiene 60.3 (1962...

...to supplement your cells with biliverdin for maximal brightness. In mammalian cells, one of the improved...Imaging Center at UCSF. A common requirement for live cell imaging experiments is the ability to follow multiple...emission spectra of the protein, so that the protein is well excited by your microscope and the fluorescence ...laboratory of Ronald Vale, after which he was a fellow at the Bauer Center for Genomics Research at Harvard...

...recognized and valued for its immense importance, it may well be what determines whether you get the job after...as an opportunity to practice communicating.  Excellent scientists learn from advisers and other mentors...forget that you have a much bigger job than merely telling your audience about the work you've done. If you...help graduate students in all STEM fields become excellent science communicators. To empower graduate students...in science communication The strength of story telling Science communication: 9 strategies to get your...

...stimulate AraC binding to I1 and I2 sites. Supplementing cell growth media with glucose decreases cAMP and represses...Steroid-regulated LexA promoter XVE (synthetic) pFZ19 (yellow flame) Temperature inducible promoters Temperature... Because transcription machinery differs between cell types or organisms, promoters are similarly variable...variable. Bacterial promoters only work in prokaryotic cells and typically only in the same or closely related... were derived. Similarly, the various eukaryotic cell types (mammalian, yeast, plants, etc.) require unique...

...can also find Dr. Page and Hello PhD on Twitter: @ThePurplePage and @HelloPhD "Role" call. #BLACKandSTEM...Science Communication” at the American Society for Cell Biology (ASCB) 2018 Conference. I don’t exactly ...Scientists SHOULD Be Using Twitter" podcast from Hello PhD In this podcast the hosts, Josh and Dan, discuss...there’s some great reminders for Twitter pros as well, like tip #4: “Follow people for about a week and...

...team potential. I often thought that performing a cell culture experiment myself would take less time than...Another team member was highly self-motivated and excelled when given the freedom to explore projects autonomously...management skills. Finally, developing emotional intelligence is vital. Learning to "read the room," understanding...part of your broader career development.   Matteo Tardelli authored the book "Beyond Academia: Stories and...molecular biology and metabolism. He now guides fellow PhDs in their careers with actionable steps and...