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We narrowed to 1,046 results for: fer

Showing: 681 - 700 of 1046 results

  1. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    ...flanking a FLOXed neoR gene. pAAV-SEPT-Acceptor, the preferred vector, contains a promoterless splice acceptor-IRES-neoR...Schofield, 2015; Moser and Hirsch, 2016; Epstein and Schaffer, 2017) Hopefully you’ve been able to check out...cohesin gene inactivation in human cancer.     References 1. Russell, David W., and Roll K. Hirata. "Human...27280971. 10. Epstein, Benjamin E., and David V. Schaffer. "Combining engineered nucleases with adeno-associated...

  2. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

    Type
    Blog Post
    ...nucleotide insertions, dele­tions or other mutations, referred to as ‘indels’. While this is convenient for generating... iPSCs (or correct them back to wild type), differentiate them into somatic cells affected by the disease...iPSC clone picking, a 100-fold decrease is the difference between picking 100 versus 10,000 clones! We ...developing novel iPSC-based model systems.     References 1. Cong, Le, et al. "Multiplex genome engineering...

  3. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    ...insertions, like a fluorescent protein knock-in, suffer from low efficiency when a standard dsDNA repair... secondary structure. Today, multiple companies offer long single-stranded DNA production services or ...Quadros et al. attribute much of this variation to differences in guide RNA efficiency. For example, in a two-guide...Quadros for assistance with editing this blog post. References Yang, Hui, et al. "One-step generation of mice...

  4. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    ...directly into fertilized mouse eggs, circumventing the need for enrichment and selection offered by mouse ...into either the pronucleus or the cytoplasm of fertilized mouse eggs. Or, to avoid handling embryos ex ...therapeutic applications that better human health.     References Cong L, Ran FA, Cox D, Lin S, Barretto R, Habib...

  5. A History of Genome Engineering in Popular Culture

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    Blog Post
    ...TALENs more specificity and a broader substrate preference than the ZFNs.   Genetic Engineering Era: Post...genetically engineered superhuman Khan. The novel, Inferno (2013) and the movie version (2016) stars Tom Hanks...that are typically associated with the DSBs and offered unprecedented prospects of curing diseases caused...your research Browse plasmids for base editing References Anzalone AV, Randolph PB, Davis JR, Sousa AA,...

  6. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

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    Blog Post
    ...nutrient-rich and also the best source for mycoplasma proliferation. Plus, it is added to the media after autoclaving... contact with a nutrient source, they start proliferating shortly. Once they are present in the laboratory...Bioluminescence assay kits are available from different biotech companies which are enzyme-based mycoplasma...and a Bachelor's degree in Biotechnology.       References Degeling MH, Maguire CA, Bovenberg MSS, Tannous...

  7. Finding Your Science Policy Path

    Type
    Blog Post
    ... away from the bench, I have worked for seven different organizations in a variety of science policy roles...cultural, and political. Science policy looks differently depending on how and where you pursue it. It ...you do science policy? Just like there are many different types of science policy careers, there are many...NSPN maintains a great resource guide including conferences, societies, and outreach groups. Grab a virtual...

  8. Expanded Access to Rabies Plasmids

    Type
    Blog Post
    ...Addgene is excited to announce we now offer an export license application for rabies materials! Some ...rabies plasmids to international requestors. We also offer export license applications for VSV and SARS-COV...before your account is charged.  Timelines  We have offered VSV and SARS-COV-1 plasmids under this application...

  9. Antibody Data Repositories and Search Engines

    Type
    Blog Post
    ...search engines, which allow you to search many different antibody catalogs at the same time.  If you're...Library for Cancer Research Human Luekocyte Differentiation Antigen Workshops Immune cells Flow cytometry...others) Data repository From the Human Cell Differentiation Molecules group Addgene Data Hub Any ...

  10. Call for Guest Bloggers

    Type
    Blog Post
    ...has deposited If you’d like to write about a different topic, feel free let us know. What do you get ...audience of biological scientists from a variety of different fields. Exposure - With over 100,000 views each...interact with and discuss your work with many different people. Recent Guest Writers Have Included: Wenning...

  12. AAV Packaged on Request is Here!

    Type
    Blog Post
    ...repository to be packaged in your choice of five different serotypes.   We are so excited for all the ways...Expanding our viral vector service Addgene has offered in-stock viral vectors since 2016, with the goal... Packaged on Request banner.   We currently offer serotypes AAV1, AAV2, AVV5, AAV8, or AAV Retrograde...

  13. Interview with Bart Newland, Addgene’s Newest Board Member

    Type
    Blog Post
    ...large companies, Genzyme and Biogen. They’re very different jobs. Being general counsel at a small start-up...companies. It's one of those "every day is something different" type of jobs, which really suits my personality... Jennifer Tsang...

  14. Bringing Sustainable Practices to the Lab: Easy Action Items

    Type
    Blog Post
    ...sustainability problem, how can one little change make a difference? Individual changes to our behavior can lead ...energy, so even one less run a week makes a big difference. Clean out freezers so you can make room and ...only better for the environment, but most likely safer for researchers, as well. Additional resources...

  15. What's New in CRISPR - Winter 2018

    Type
    Blog Post
    ... been difficult to compare the performance of different nucleases. Nicola Patron’s lab compared the efficiency...genome editing toolkit New Cas protein identified Jennifer Doudna’s lab recently identified Cas14, a family... Jennifer Tsang...

  16. MassBioEd Career Exploration Day at Addgene

    Type
    Blog Post
    ...learn about intellectual property from our Tech Transfer team members Tony Cova and Chiara DeNatale and...of the students were interested to see so many different career options in science, with Abigail Anderson...professor. There are amazing opportunities that are offered with a biology major.” After hearing about many...

  17. Your Top Requested Plasmid in 2015!

    Type
    Blog Post
    ... name suggests, lentiCRISPR v2 is a lentiviral transfer vector used to deliver both Cas9 and your gRNA...metalloproteinase-9 secretion, and identifying lysine methyltransferase substrates. We’re incredibly happy that lentiCRISPR... forward to another great year of sharing!   References 1. Shalem, Ophir, et al. "Genome-scale CRISPR-Cas9...

  18. Inntags: Innovative Protein Epitope Tagging

    Type
    Blog Post
    ...antibody. There is one major caveat - a tag may interfere with protein localization and/or function, so ... like FLAG, HA, and Myc can adopt a number of different conformations and thus potentially interact with...our older epitope tags - only time will tell! References 1. Georgieva, Maya V., et al. "Inntags: small...

  19. More Data for You: Find Articles Citing Addgene Plasmids

    Type
    Blog Post
    ...validated in.  If a plasmid's Addgene ID # has been referenced in other publications, you'll find a link to ...Additional features Once you've clicked on the "# References" link under the "Resource Information" heading...Addgene’s Plasmids 101 eBook Protocols and Plasmid References @Addgene  ...

  20. Science Communication for Everyone

    Type
    Blog Post
    ... how our articles have helped them understand different topics of science, especially disease related ...terms like manipulations, anomaly, and so on, differently than scientists would. For example, “manipulate... receive our science career guide Learn about different careers in science communication Resources on...
Showing: 681 - 700 of 1046 results