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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 1,041 results for: ins;

Showing: 681 - 700 of 1041 results

  1. Deep Dive: qPCR

    Type
    Blog Post
    ...used GAPDH has noted issues as a reference gene (Higgins, 2000; Ref, Zhu, 2002), though there are cases ...run and the difference in CT values is plotted against the log input concentration for each dilution. ...://doi.org/10.1038/s41598-018-26707-8Suzuki T; Higgins PJ; Crawford DR. Control selection for RNA quantitation...

  2. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    ...a template. To do so, they used a Cas nickase, instead of dCas9. The resulting editor, BE3, nicks the ...and/or evolved to catalyze cytidine deamination instead of (or in addition to) adenosine deamination (Chen... R. T., Christie, K. A., Whittaker, M. N., & Kleinstiver, B. P. (2020). Unconstrained genome targeting...

  3. Addgene is Stopping Use of X (formerly Twitter)

    Type
    Blog Post
    ...Bluesky: @addgene.bsky.social; or @addgene on Instagram, TikTok, Facebook, and LinkedIn. We are also exploring...and features that previously made the platform a mainstay of conversation in the scientific community. Addgene...

  4. #Deckthelab Contest Winners 2017

    Type
    Blog Post
    ...of our holiday decorations, our holiday spirit remains. We would like to thank everyone who participated...creating memories with their labmates! Are you inspired by our 2017 #Deckthelab winners? Be sure to enter...

  8. 8 Great Depositor Research Stories

    Type
    Blog Post
    ...Goldilocks Antibody: Guest blogger Ava Shipman explains how the Wu lab is screening over 3,000 antibodies... a drag-and-drop CRISPR editing tool for large inserts.  DAP arrays for multiplex gene editing: Guest ...

  9. Celebrating the 2024 Nobel Prize in Chemistry

    Type
    Blog Post
    ...The Baker Lab focuses on designing entirely new proteins, improving our understanding of how protein folding...and others. The introduction of de novo designed proteins also helped researchers understand more about ...

  10. Introducing Addgene's Microbiology Pages

    Type
    Blog Post
    ...Plasmid Kits for microbial work with fluorescent proteins, synthetic biology, gene cloning and expression...them on our search page. Use the Expression and Insert Species filters on the right-hand side of the search...

  11. CRISPR Cheat Sheet

    Type
    Blog Post
    ...to cut other DNA or RNA sequences. Nucleases: Proteins that bind to gRNAs and are directed by the gRNAs...prime editing and the identification of other Cas proteins continue to expand the targeting range. CRISPR...

  13. Countdown to Halloween @Addgene

    Type
    Blog Post
    ...tradition in which we choose our teams and start brainstorming months in advance. Our teams comb the news (...Friday. So if you’re interested in finding out who wins this year’s Addgene costume contest, like us on ...

  14. Podcast: Interview with Addgene's Founders

    Type
    Blog Post
    ...and metabolism. Melina has worked at Genetics Institute in Cambridge, MA and Sequana Therapeutics in San...the Addgene Blog Addgene's a nonprofit? Get an Inside Look at Addgene's NGS Quality Control Addgene's...

  15. Newly Updated AAV Data Hub!

    Type
    Blog Post
    ... share their AAV validation data. The Data Hub contains a curated collection of community submissions ...) can save you significant time and money. For instance, this report shows that anterograde, monosynaptic...

  16. Editor's Choice, October 2016

    Type
    Blog Post
    ...academic institutions can afford to hire. It will be difficult for some of these institutions to manage...

  17. Editor's Choice, July 2016

    Type
    Blog Post
    ... about how fluorophores other than fluorescent proteins can be incredibly useful in FRET experiments. ... our posts if you have any questions or useful insights. If you're interested in blogging for us, fill...

  18. Bringing Sustainable Practices to the Lab: Easy Action Items

    Type
    Blog Post
    ...probably bring in a mug or reusable water bottle instead of buying yourself a big stack of plastic cups....could be: Setting up a recycling program at your institute. Set your -80 °C freezer to -70 °C and teach other...

  19. The Addgene Antibody Data Hub

    Type
    Blog Post
    ...submission is used to create a data report, which contains experimental data showing how the antibody performed...immunocytochemistry, ELISA — but in all cases, it contains a detailed description of the experimental conditions...

  20. Grad School Advice Part 2: Building Community

    Type
    Blog Post
    ...Sciences program at Harvard University and pick their brains on how they've managed to keep themselves happy...year dip (see graph). Niroshi and Ben give their insights as to what causes this dip. 11:11 - 15:37 : How...
Showing: 681 - 700 of 1041 results