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We narrowed to 971 results for: lars

Showing: 681 - 700 of 971 results

  1. Deep Mutational Scanning with One Pot Saturation Mutagenesis

    Type
    Blog Post
    ...functional screen of your liking. This method is similar to site-directed mutagenesis (SDM) PCR, but with...Random Mutagenesis by Error-Prone PCR”. Methods in Molecular Biology In Vitro Mutagenesis Protocols, pg. 103...Measuring the Activity of Protein Variants on a Large Scale Using Deep Mutational Scanning.” Nature Protocols...

  2. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    ..., but I always seek out information about the particular audience and venue before tailoring each presentation...peer mentors to check your slides for typos and clarity in a big room.  Finally, don’t pass up opportunities...informal poll by asking for a show of hands on a particular question. I like to ask about how many people...

  3. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    ...efficiency differed from 48-100% between strains. In a similar experiment, CRISPR reduced the HIV-1 copy number...evidence that an HIV-1 cure may be within our reach. Similar studies have shown that CRISPR can be used to combat...Additional Resources on the Addgene Blog Treating Muscular Dystrophy with CRISPR Gene Editing Learn to Use...

  4. Molecular Biology Reference

    Type
    Guide
    ...Educational Resources Molecular Biology Reference Molecular Biology Reference Jump To: Molecular Biology Basics...Addgene's molecular biology reference guide, including information on molecular genetics, plasmids, sequencing... molecule is known as DNA or molecular cloning . The process of molecular cloning enabled scientists to...Basics Origins of Molecular Genetics Plasmid Elements Working with Plasmids Introduction Types of Plasmids...Webpage and Blog References Protocols Origins of Molecular Genetics The concept of genes as carriers of phenotypic...These findings culminated in the central dogma of molecular biology, that proteins are translated from RNA...chemistry enable isolation and purification of cellular components, such as DNA, but practically this ...

  5. Coomassie Purity Stain of Recombinant Antibodies

    Type
    Protocol
    ...and reagents from other vendors should produce similar outcomes when using these protocols. However, please...blade Plastic tray Gel imaging system ImageJ or similar software Reagents Purified recombinant antibody...should be very little background staining. Pro-Tip A large shift in the electrophoretic mobility of the bands...Analysis Use ImageJ (Link opens in a new window) or a similar photo software to determine the relative intensity...

  6. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    ... to choose from that sequence preferences must largely be ignored. Created with BioRender.com. ...nuclease-mediated off-target effects.” Further, large-scale datasets of hundreds of genetic screens using...Tsherniak A, Iorio F (2019) Agreement between two large pan-cancer CRISPR-Cas9 gene dependency data sets...

  7. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    ...appropriate secondary antibodies. The general process is similar to standard immunofluorescence, but with some extra...selecting an antibody, some of the requirements are similar to standard IF. Here, we’ll walk you through the...if the localization and intensity patterns are similar between the two experiments. If not, it might be...

  8. Building Global Connections with the International Mentorship Program USA-EUROPE

    Type
    Blog Post
    ...Mentorship Program work? IMP Pilot Program 2015-2015 Scholars need strong proactive mentors who have relevant...President from the non-profit IMFAHE, Inc. She is particularly interested in breast cancer and metastasis as... Medical School, where she studies age-related macular degeneration. She is also a blogger who writes ...

  9. AAV ddPCR Titration

    Type
    Protocol
    ... Service Viral Vector Guides Virus Blog Posts Molecular Biology Reference Introduction This protocol describes... lower or higher dilutions depending on their particular sample. For additional tips on AAV titering using... bucket 96-well freezer blocks (x 3) Reagents Molecular Biology Grade Water, Hyclone, SH30538.02 GeneAmp...10X Buffer 25 µL of 10% Pluronic F-68 4475 µL Molecular Biology Grade Water Procedure Preparation Before...

  10. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    ...reason most people enter postdoctoral training. Similarly, there are several factors to consider when selecting...to strengthen your new model?  PFs need to be regularly thinking about manuscripts. The other items will...Erik Snapp! Erik Lee Snapp received his PhD in Molecular Microbiology and Immunology from Oregon Health...

  11. Protocol - How to Purify DNA from an Agarose Gel

    Type
    Protocol
    ...samples. This is a commonly used technique for molecular cloning, such as PCR - or restriction enzyme -...you get better separation of bands? If you have similarly sized bands that are running too close together..., and a lower percentage gel will help separate larger bands. 10% Rule For each sample you want to load...

  12. Protocol - How to Perform a Diagnostic Digest

    Type
    Protocol
    ...result in similar sized bands, thus making this simple digest less informative. This is particularly true ...occurring bacterial endonucleases that recognize a large range of DNA sequences. Given the variety of these...

  13. Protocol - How to Inoculate a Bacterial Culture

    Type
    Protocol
    ...usually want to start 2 mL in a falcon tube, but for larger preps you might want to use as much as a liter ...individual plasmid within a single bacterial cell. Large plasmids usually have a low copy number (approximately... other hand, smaller plasmids can be present in large numbers, 50 or more per cell and have a high copy...

  14. Protocol - Bacterial Transformation

    Type
    Protocol
    ...less efficient at taking up larger plasmids. If you need to transform large plasmids, it is a good idea...very few if any transformants when transforming large plasmids (>10 kb) or BACs, what can I do? Chemically...

  16. New FLIS Plasmid (April Fools 2022)

    Type
    Blog Post
    ...been an important animal model for research, particularly genetics research, for decades. Its short life...bright burst of light, visible to the naked eye, at regular intervals. This innovation system can help fly ...

  19. Pipetting Protocol

    Type
    Protocol
    ...pipette is an essential tool for nearly anyone in molecular biology. You’ll use a pipette in many scenarios... liquid. Pro-Tip If pipetting from a relatively large container holding a small volume of liquid, tilt... of and gently in contact with the container (capillary action between the container and the liquid will...

  20. Transfection for Recombinant Antibodies

    Type
    Protocol
    ...Antibody Plasmid Collection Antibodies Blog Posts Molecular Biology Reference General Transfection Protocol...and reagents from other vendors should produce similar outcomes when using these protocols. However, please...replication use a HEK293 line stably expressing the SV40 large T antigen. For antibody-expressing plasmids containing...
Showing: 681 - 700 of 971 results