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  1. Addgene Depositors Get More Citations

    Type
    Blog Post
    ...Addgene plasmids and those without, we adjust our estimates based on the journal that published the articles... This is also true for other journals, so our estimates now take into account any differences in citations...

  2. The Power Behind NGS Plasmid Validation: seqWell

    Type
    Blog Post
    ... of a 96-well plate. Then, samples from several plates are pooled together to create a single library ... from plexWell™ NGS data. Once Addgene produces plates of plasmid DNA, they are shipped to seqWell, where...

  3. Science Career Options

    Type
    Blog Post
    ...writing skills Writing a thesis or dissertation demonstrates that you have advanced writing and formatting...apply for a job, it is useful to search online job websites to see what requirements or qualifications are...

  5. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    ...short, K. phaffii is a non-pathogenic yeast that operates as an obligate aerobe. It’s also a methylotropic...has been sequenced for some time, clearing the greatest hurdle for genome engineering. A unique evolutionary...

  10. Plasmids 101: Gram Negative and Positive Bacteria

    Type
    Blog Post
    ...layer of both bacteria types. In water, the dye dissociates into CV+ and Chloride (Cl-) ions. When the negatively...the cell purple. When the alcohol is added, it dehydrates the peptidoglycan layer, trapping the large dye...

  11. With Our New Viral Service, We're Taking CRISPR Further!

    Type
    Blog Post
    ...alleviate this frustration by producing, titering, testing, and providing lentiviral preps of select lentiviral... vectors from the repository for you. We perform test infections with all of our viral preps and host ...

  12. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    ...dynamically rearranging into different polymerization states. Instead, R body monomers respond to pH changes...their cell walls removed with lysozyme). This demonstrates that R bodies can function to break membranes...

  13. Addgene Welcomes John Doench to the Advisory Board

    Type
    Blog Post
    ... up on a Saturday at like 7 am to go check your plates and you’re excited to do so, you know that that...Addgene has behind it is something that very much resonates with me.” About Addgene’s role in the future,...

  15. Fluorescent Proteins 101: Luciferase

    Type
    Blog Post
    ...reaction requiring luciferin, ATP and oxygen as substrates. Production of photons by this bioluminescent...of Photon Generation Slower Faster Cofactors/Substrates Required Not Required Signal Strength Lower...

  17. Clear and Accessible Writing

    Type
    Blog Post
    ...supernatant through each layer. The right panel illustrates a gradient tube before and after ultracentrifugation...ultracentrifugation. The viral supernatant migrates through increasingly dense layers until the macromolecule density...

  19. Addgene’s Viral Service - Why Virus? Why Now?

    Type
    Blog Post
    ... and have worked closely with us to functionally test our virus. Ed Boyden’s lab from MIT and Alla Karpova... methods, performs extensive quality control, generates  educational materials, and provides viral vector...

  20. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    ...security-critical bugs, and contribute to constant updates of the software. In hardware, this process is often...are as follows: Detailed documentation, which facilitates the reproduction of the structure in a similar...
Showing: 681 - 700 of 955 results