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We narrowed to 955 results for: tes

Showing: 681 - 700 of 955 results

  3. Plasmids 101: Gram Negative and Positive Bacteria

    Type
    Blog Post
    ...layer of both bacteria types. In water, the dye dissociates into CV+ and Chloride (Cl-) ions. When the negatively...the cell purple. When the alcohol is added, it dehydrates the peptidoglycan layer, trapping the large dye...

  4. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    ...short, K. phaffii is a non-pathogenic yeast that operates as an obligate aerobe. It’s also a methylotropic...has been sequenced for some time, clearing the greatest hurdle for genome engineering. A unique evolutionary...

  7. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    ...dynamically rearranging into different polymerization states. Instead, R body monomers respond to pH changes...their cell walls removed with lysozyme). This demonstrates that R bodies can function to break membranes...

  8. Addgene Welcomes John Doench to the Advisory Board

    Type
    Blog Post
    ... up on a Saturday at like 7 am to go check your plates and you’re excited to do so, you know that that...Addgene has behind it is something that very much resonates with me.” About Addgene’s role in the future,...

  11. Addgene’s Viral Service - Why Virus? Why Now?

    Type
    Blog Post
    ... and have worked closely with us to functionally test our virus. Ed Boyden’s lab from MIT and Alla Karpova... methods, performs extensive quality control, generates  educational materials, and provides viral vector...

  12. GPCRs: How Do They Work and How Do We Study Them?

    Type
    Blog Post
    ...parts: the receptor itself and the G-proteins it associates with.  Receptor: GPCRs are also known as seven...transducing receptors in the brain they are ideal candidates for use in chemogenetics. To create chemogenetics...

  13. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    ...site of interest, which nCas9 nicks and then dissociates from. PolI3M then binds the nicked DNA, and extends..., where it nicks its target DNA (left). Cas9 dissociates and PolI binds (middle) and extends from the ...

  14. Plasmids 101: Screens vs. Selections

    Type
    Blog Post
    ...small molecule realm is typically not feasible to test in any given screening experiment. Thus, a limited...require there to be a culture condition which eliminates manipulations that do not affect the function...

  15. Antibodies 101: ChIP

    Type
    Blog Post
    ...  Fig. 1: Schematic overview of ChIP. Image courtesy of Shengliu via Wikicommons.   Okay, let’s...celebrating a successful ChIP experiment! Image courtesy of https://www.wannapik.com/   Even though...

  16. Hot Plasmids - February 2022

    Type
    Blog Post
    ...2: comparison of Cas 9 vs. Cas 12a expression cassettes when encoding multiple guides. While two guides... and fusion proteins, such as Cas9 and Cas12a, creates challenges for in vivo delivery and cell engineering...

  18. Clear and Accessible Writing

    Type
    Blog Post
    ...supernatant through each layer. The right panel illustrates a gradient tube before and after ultracentrifugation...ultracentrifugation. The viral supernatant migrates through increasingly dense layers until the macromolecule density...

  19. Viral Vectors 101: Inverted Terminal Repeats

    Type
    Blog Post
    ...sequence containing transcription factor binding sites can increase transgene expression and transduction...validate ITR sequences as these enzymes cut at known sites within the ITR. However, small deletions and point...

  20. OCaMP: A New Calcium Indicator for Neural Imaging

    Type
    Blog Post
    ...Better performance = better data In side-by-side tests with popular red and yellow GECIs, OCaMP shows: ...events are indicated below the trace; asterisk indicates a single AP. B) Averaged fluorescence responses...
Showing: 681 - 700 of 955 results