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Showing: 681 - 700 of 963 results
  1. Advice for Moving Into Sales After Your Science Postdoc

    Type
    Blog Post
    ... for marketing positions posted on recruitment websites, this job specifically looked for a researcher...think of to start was “Marketing for Dummies”. The fastest way to learn something about marketing would be...
  2. Addgene Depositors Get More Citations

    Type
    Blog Post
    ...Addgene plasmids and those without, we adjust our estimates based on the journal that published the articles... This is also true for other journals, so our estimates now take into account any differences in citations...
  3. The Power Behind NGS Plasmid Validation: seqWell

    Type
    Blog Post
    ... of a 96-well plate. Then, samples from several plates are pooled together to create a single library ... from plexWell™ NGS data. Once Addgene produces plates of plasmid DNA, they are shipped to seqWell, where...
  4. Science Career Options

    Type
    Blog Post
    ...writing skills Writing a thesis or dissertation demonstrates that you have advanced writing and formatting...apply for a job, it is useful to search online job websites to see what requirements or qualifications are...
  5. Plasmids 101: Gram Negative and Positive Bacteria

    Type
    Blog Post
    ...layer of both bacteria types. In water, the dye dissociates into CV+ and Chloride (Cl-) ions. When the negatively...the cell purple. When the alcohol is added, it dehydrates the peptidoglycan layer, trapping the large dye...
  6. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    ...short, K. phaffii is a non-pathogenic yeast that operates as an obligate aerobe. It’s also a methylotropic...has been sequenced for some time, clearing the greatest hurdle for genome engineering. A unique evolutionary...
  7. With Our New Viral Service, We're Taking CRISPR Further!

    Type
    Blog Post
    ...alleviate this frustration by producing, titering, testing, and providing lentiviral preps of select lentiviral... vectors from the repository for you. We perform test infections with all of our viral preps and host ...
  8. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    ...dynamically rearranging into different polymerization states. Instead, R body monomers respond to pH changes...their cell walls removed with lysozyme). This demonstrates that R bodies can function to break membranes...
  9. Addgene Welcomes John Doench to the Advisory Board

    Type
    Blog Post
    ... up on a Saturday at like 7 am to go check your plates and you’re excited to do so, you know that that...Addgene has behind it is something that very much resonates with me.” About Addgene’s role in the future,...
  10. Antibody Validation Using the Indirect ELISA Method

    Type
    Protocol
    ...of our protocols supports reproducibility and accelerates science. Here, we list the specific equipment...instead of room temperature or 37 °C. The protocol notes when there are different incubation options available...empirically determined. If possible, run an initial test with serially diluted primary antibody and determine...Equipment Spectrophotometer compatible with 96-well plates 1–10 µL single channel pipette 2–20 µL single channel...VWR 76322-150 Pipette tips, 20 µL, VWR 76322-134 Pipettes, 10 mL, VWR 89130-898 1 L polystyrene bottle, ...display a green product. A precipitated product indicates the presence of too much HRP and the need to optimize... and incubated overnight with Anti-Desmin [D7]. Plates were incubated with HRP-linked anti-Human IgG secondary...
  11. OCaMP: A New Calcium Indicator for Neural Imaging

    Type
    Blog Post
    ...Better performance = better data In side-by-side tests with popular red and yellow GECIs, OCaMP shows: ...events are indicated below the trace; asterisk indicates a single AP. B) Averaged fluorescence responses...
Showing: 681 - 700 of 963 results