We narrowed to 916 results for: tro
-
TypeBlog Post...world and corrective approaches and appropriate controls are required to make this method useful for dynamic...measurements. Extra care should be taken so as not to destroy the donor molecule. Fluorescence lifetime imaging...
-
SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas
TypeBlog Post...evident that widespread testing is crucial in controlling this pandemic. Currently, the CDC uses RT-qPCR... need for thermocyclers. This is followed by in vitro transcription and Cas13 is then used to detect the... -
Antibodies 101: Beyond Surface Labeling
TypeBlog Post...alongside standard surface labels. Here, I will introduce you to a few useful labeling and staining techniques...Reading a Flow Plot Antibodies 101: Flow Cytometry Controls Antibodies 101: Validation... -
Tips for Getting a Faculty Position
TypeBlog Post...If you read my eBook and it helps you, please introduce yourself to me by email or in person. If you feel...Medical Institute. His interests include the quality control of secretory proteins in the Endoplasmic Reticulum... -
10 Steps to a Perfect Science Talk
TypeBlog Post...Like graphing data, choosing controls, or mixing clear solutions—public speaking is skill that any scientist...women), visibility (how to get it, especially if introverted ), and networking (how to get people to remember... -
Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods
TypeBlog Post...exonuclease feature of T4 DNA polymerase, which must be controlled by the presence or absence of dNTPs. Secondly...assembly a ligase is added to repair the nicks in vitro, whereas in SLIC these constructs are repaired in... -
CRISPR 101: Epigenetics and Editing the Epigenome
TypeBlog Post...Epigenetic modifications are an additional layer of control over gene expression that go beyond genomic sequence...catalytically dead modifiers are also available as controls. For an up-to-date list of CRISPR epigenetic tools... -
Technologies Enabled by NanoLuc® Luciferase
TypeBlog Post...constructs were designed to be packaged into a retrovirus for infection of host cells. The fluorescent ... is composed of Venus fluorescent protein, the Troponin C Ca++ binding domain, and NanoLuc® Luciferase... -
Mycoplasma Contamination: Where Does It Come From and How to Prevent It
TypeBlog Post...culture spilled immediately. Water baths, liquid nitrogen containers, reagent bottles and other sources ...Mycoplasma Infections from Continuous Cell Lines. In Vitro Cell Dev Biol Anim 38:86 . https://doi.org/10.1290... -
Finding Your Science Policy Path
TypeBlog Post...backgrounds with technical expertise that ranges from astronomy to bioethics to chemical engineering and everything...policy training programs and fellowships have a strong focus on early career researchers who have decided... -
A Guide to Getting Started in Undergrad Research
TypeBlog Post...Research requires dedication and effort, so building a strong understanding of what you’re getting yourself into... what I don’t know yet has helped me cultivate stronger relationships with my labmates. Sometimes what... -
CRISPR 101: Cytosine and Adenine Base Editors
TypeBlog Post...specify and correct single-base mutations without introducing DSBs at all. Base editing to the rescue! Base...editor. Dotted red arrows indicate the change introduced to the opposite strand following base editing... -
New Antibodies in the Lab Animation!
TypeBlog Post...immunocytochemistry, and flow cytometry. It's a great introduction to — or quick refresher on — these common antibody... -
Plasmids 101 eBook 4th Edition
TypeBlog Post...nomenclature to popular cloning techniques and troubleshooting tips. The fourth edition has been fully updated... -
Beginner's Guide to Viral Vectors
TypeBlog Post...some of the most important characteristics of retroviruses, lentiviruses, AAVs, and adenoviruses in this... -
Viral Vectors 101 eBook 2nd Edition
TypeBlog Post...scientists everywhere. In it, you can find an introduction to viral vectors and their elements; resources... -
Antibodies 101 Series on TikTok
TypeBlog Post...resources on the Addgene blog Antibodies 101: Introduction to Antibodies New Antibody Animation ... -
CRISPR Guide Update
TypeBlog Post...Please check out the updated guide for a great introduction to what CRISPR technologies have to offer. Happy... -
Meet Aavery, Blugene's New Virus Friend!
TypeBlog Post...Aavery! Original post: In December, we introduced you to Blugene’s new virus friend (they/them/... -
We’re Going Viral This Week!
TypeBlog Post...We first “went viral” in 2016 when we introduced the viral service, and now we’re “going viral” again...