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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 922 results for: tro

Showing: 681 - 700 of 922 results

  3. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    ...defective chloramphenicol resistance gene (CamR) by introducing a point mutation (H193Y). The reversion of this...promoter driven expression system. We use a single polycistronic tRNA-gRNA (PTG) gene system to clone multiple...

  4. Transferable Skills Guide: Career Planning Resources

    Type
    Blog Post
    ...that are more career-focused. After these talks, introduce yourself to the speaker and offer your business...them for work. Addgene’s very own Joanne Kamens, a strong advocate for career planning, has written a great...

  5. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    ...world and corrective approaches and appropriate controls are required to make this method useful for dynamic...measurements. Extra care should be taken so as not to destroy the donor molecule. Fluorescence lifetime imaging...

  6. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    ...alongside standard surface labels. Here, I will introduce you to a few useful labeling and staining techniques...Reading a Flow Plot Antibodies 101: Flow Cytometry Controls Antibodies 101: Validation...

  7. Tips for Getting a Faculty Position

    Type
    Blog Post
    ...If you read my eBook and it helps you, please introduce yourself to me by email or in person. If you feel...Medical Institute. His interests include the quality control of secretory proteins in the Endoplasmic Reticulum...

  8. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    ...evident that widespread testing is crucial in controlling this pandemic. Currently, the CDC uses RT-qPCR... need for thermocyclers. This is followed by in vitro transcription and Cas13 is then used to detect the...

  9. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    ...Like graphing data, choosing controls, or mixing clear solutions—public speaking is skill that any scientist...women), visibility (how to get it, especially if introverted ), and networking (how to get people to remember...

  11. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    ...constructs were designed to be packaged into a retrovirus for infection of host cells. The fluorescent ... is composed of Venus fluorescent protein, the Troponin C Ca++ binding domain, and NanoLuc® Luciferase...

  12. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    ...Epigenetic modifications are an additional layer of control over gene expression that go beyond genomic sequence...catalytically dead modifiers are also available as controls. For an up-to-date list of CRISPR epigenetic tools...

  13. Antibodies 101: Antibody Engineering and Directed Evolution

    Type
    Blog Post
    ...Structure-guided directed evolution If you desire a bit more control, structure guided directed evolution might be more...evolution, all while maintaining a higher degree of control and efficiency! Screening mutant libraries After...

  14. A Guide to Getting Started in Undergrad Research

    Type
    Blog Post
    ...Research requires dedication and effort, so building a strong understanding of what you’re getting yourself into... what I don’t know yet has helped me cultivate stronger relationships with my labmates. Sometimes what...

  16. Finding Your Science Policy Path

    Type
    Blog Post
    ...backgrounds with technical expertise that ranges from astronomy to bioethics to chemical engineering and everything...policy training programs and fellowships have a strong focus on early career researchers who have decided...

  17. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    ...specify and correct single-base mutations without introducing DSBs at all. Base editing to the rescue! Base...editor. Dotted red arrows indicate the change introduced to the opposite strand following base editing...

  18. New Antibodies in the Lab Animation!

    Type
    Blog Post
    ...immunocytochemistry, and flow cytometry. It's a great introduction to — or quick refresher on — these common antibody...

  19. Plasmids 101 eBook 4th Edition

    Type
    Blog Post
    ...nomenclature to popular cloning techniques and troubleshooting tips. The fourth edition has been fully updated...
Showing: 681 - 700 of 922 results