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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,010 results for: RING;

Showing: 351 - 360 of 1010 results

  1. Hot Plasmids: Fall 2024

    Type
    Blog Post
    ...But CRBN has been difficult to study in vitro, requiring either expressing it with additional cofactors...faster, with expression levels comparable to GFP during early embryogenesis (Figure 3). mScarlet-I3 also... through the middle of each embryo are shown, comparing GFP, wrmScarlet (mScarlet), and the new mScarlet3...proteins of interest) and mScarlet-I3 for scenarios requiring rapid maturation and prolonged imaging with minimal...high rate of spontaneous antibiotic resistance interfering with selection. Using an inducible Cas9 expression...to cause disruption of the target gene. Lastly, curing the mCherry-sgRNA plasmid leaves an edited strain...

  2. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    ...the Wyss Institute for Biologically Inspired Engineering. CRISPR/Cas9 is an enormously plastic tool and...activator by simply providing a new gRNA rather than engineering an entirely new protein. This makes it much cheaper...types of Cas9 activators, check out our blog post covering VP64, Suntag, SAM, and VPR. Worry less about off...the Wyss Institute for Biologically Inspired Engineering.     Alejandro (Alex) Chavez is a member of the...develop several popular CRISPR-based tools for altering DNA sequence or regulating gene expression.   ...Church GM (2015) Unraveling CRISPR-Cas9 genome engineering parameters via a library-on-library approach....

  3. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    ...optimization? Codon optimization is an approach in gene engineering to improve gene expression by changing synonymous...efficiency and thus protein expression without altering the sequence of the protein. As DNA synthesis ...tRNAs, including those that exhibit wobble base-pairing (a tRNA that can recognize multiple synonymous ...of translation. The ribosome may slow and pause during elongation which may actually be necessary for ... SA, Zhou W, Mauro VP (2005) An mRNA-rRNA base-pairing mechanism for translation initiation in eukaryotes...02)00362-x Stadler M, Fire A (2011) Wobble base-pairing slows in vivo translation elongation in metazoans...

  4. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    ...inteins. Split inteins are a pair of naturally occuring polypeptides that, when at the ends of two proteins...Marraffini LA, Zhang F (2013) Multiplex Genome Engineering Using CRISPR/Cas Systems. Science 339:819–823...Spinner HB, Baney KLM, Chuck J, Tan D, Knott GJ, Harrington LB, Al-Shayeb B, Wagner A, Brötzmann J, Staahl..., Grimm D (2014) CRISPR/Cas9-mediated genome engineering: An adeno-associated viral (AAV) vector toolbox...Gao L, Makarova KS, Koonin EV, Zhang F (2019) Engineering of CRISPR-Cas12b for human genome editing. Nat... Mary Gearing...

  5. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    ...interested in measuring a population based on a fluorescent stain, you might be wondering which second ...types of scattering that occur when a cell is pulsed by the laser. Within each type of scattering, you also...to keep it short though, promise! FSC and SSC: scattering To digitally extract your cells based on their...cells stain positively for both markers. When comparing two markers simultaneously, we commonly describe...

  6. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    ...about the particular audience and venue before tailoring each presentation appropriately.  Find out who...many non-scientists be present?  Always ask the hiring manager or host for topic advice. An added bonus...figure out how to make it suspenseful. 4. Start preparing your talk early - like today Don’t wait until ... all graduate students and postdocs form peer mentoring groups to practice giving talks and getting feedback...always have a few extra slides in their back pocket. During your practice rounds, encourage your listeners ...down it is a great segue to talk about how the sharing model works. See if you can elicit helpful comments...

  7. Replacing Paper: Tips for Choosing an Electronic lab Notebook

    Type
    Blog Post
    ...briefing, to quick notes, to finances, to flights & ordering food - everything is covered.   So why are we ...folders in complex ways, there are solutions that bring more to the table in terms of data traceability ...researchers today generate vast amounts of digital data during their work. It is already becoming impossible to...to keep all their data in one place and avoid scattering intellectual property across various software...functionalities include comments, notifications, sharing files, assigning team members to certain tasks,...compliance requirements – e.g. 21 CFR part 11, HIPAA? Answering this question helps you evaluate whether the ELN...

  8. Editor's Choice, October 2016

    Type
    Blog Post
    ...important information for anyone considering a postdoc. In “Changing Labor Laws Bring Increased Postdoc Wages,...readership with over 75,000 views. Changing Labor Laws Bring Increased Postdoc Wages Our most popular blog post...Grad School Advice Part 2: Building Community Engineering the Plant Genome Using CRISPR/Cas9 Healthcare...Reddit for Science Communication Changing Labor Laws Bring Increased Postdoc Wages I hope you all have a great...

  9. 2022 and 2023: Reflections and Looking Forward

    Type
    Blog Post
    ... what challenges remain in materials sharing, information sharing, and research reproducibility while ...service and support.  Alongside our material offerings, in 2022 we also rolled out an updated AAV Data...around the world can upload AAV validation data, sharing what works and, equally importantly, what doesn...commitment to supporting the research community through sharing science and distributing high-quality materials...

  10. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    ...relatively simple in C. elegans and bacteria. But CRISPRing typical mouse models, including creating Cre-dependent...Megamer service tested by Quadros et al. When delivering the ssDNA template and CRISPR machinery, Quadros...facilities for 13 projects, with most projects requiring only 40-60 zygotes to generate a correctly edited...conditional alleles by CRISPR/Cas-mediated genome engineering." Cell 154 (2013):1370-9. DOI: 10.1016/j.cell... a robust method for in situ germline genome engineering using CRISPR nucleases. Genome Biol. 19 (2018... Mary Gearing...
Showing: 351 - 360 of 1010 results