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Showing: 701 - 720 of 1019 results

  1. Viral Vectors 101: Inverted Terminal Repeats

    Type
    Blog Post
    ...Adeno-associated viruses (AAV) are an important biological tool that allow scientists to express a gene ...with biorender.com. ITR structure  WT ITRs are symmetrical 145-nucleotide sequences that flank the ends ...serotypes vary in their gene transfer efficiency. Our practical knowledge of ITRs is still limited, but this area...

  2. Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies

    Type
    Blog Post
    ...study it. The process is fairly straightforward. Basically, you place your gene of interest in a plasmid ...genetic crosses with balancer chromosomes to make genetically stable fly lines. After a few weeks of waiting... aware of at least some of the possibilities. Basically, if you can devise an experimental assay that ...

  3. Addgene’s Viral Service - Why Virus? Why Now?

    Type
    Blog Post
    ...North Carolina, Connie Cepko’s lab from Harvard Medical School, and Jonathan Ting’s lab from the Allen ...educational materials, and provides viral vector technical service (details about virus production protocols...word out about this new resource and providing fantastically useful educational content on viral vectors....

  4. Antibodies 101: Isotypes

    Type
    Blog Post
    ...chain constant regions result in several unique physical characteristics of these five isotypes, such as...their heavy chain constant domains. These are typically numbered, i.e. IgG1, IgG2, etc. Let’s dive in ...different constant domain gene. This process is called isotype switching and results in the B cell expressing...

  7. Behind the scenes: Addgene’s new search engine and more

    Type
    Blog Post
    ..., a powerful open-source search engine that can scale to search millions of web resources and handle millions..., partial words, phrases, partial phrases, or numerical identifiers. We also combine the different terms...PI labs and institutions. There are many more technical details that make our new search engine unique...

  9. Clear and Accessible Writing

    Type
    Blog Post
    ... reader accessing the information? This can be physical or digital. For instance, you would present information...most helpful to use a lot of jargon and have a technical, detailed approach. Other times, it is more helpful...thought it would be most helpful to highlight the technical details of collecting the purified AAV–that the...

  10. AAV Q&A with Tim Miles

    Type
    Blog Post
    ...Tim Miles, PhD, Director of the CLOVER Center at CalTech? If so, you may have submitted a question that ...Have you or others been able to successfully systemically transduce and then retransduce a model organism...as multiple sclerosis?  A: Basic science and preclinical researchers have used the flexibility of AAV ...

  11. Plasmids 101: Methylation and Restriction Enzymes

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    Blog Post
    ...restriction endonucleases. A given prokaryote typically has genes encoding one or a few restriction modification...Well, even though Dam methylation sites are not specifically associated with any restriction modification... These specialized E. coli strains have been specifically engineered to be Dam and Dcm methylase-deficient...

  12. Human Germline Editing Using CRISPR

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    Blog Post
    ... rate would need to be much, much lower before clinical applications could be considered. It is important... efficiency and applicability. Given moral and ethical concerns surrounding such research, it is crucial... experiments are needed before we can consider clinical applications for germline editing. Although this...

  13. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

    Type
    Blog Post
    ...reprograming influence tumors development? Being pathological active regions, microenvironments shape a tumor...interested in science journalism and writing about biomedical issues. Follow her on Twitter @BrbaraPinho6. ...expression predicts early tumor recurrence and poor clinical outcome in human breast cancers." The American...

  14. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type
    Blog Post
    ...developed cloning vectors to harness it. These vectors, called pKIL18 and pKIL19, contained the ccdB gene in frame...ccdB and therefore would die. This procedure dramatically reduces the number of clones that do not contain...and ccdB Survival strains have been developed specifically with ccdB-containing vectors in mind, any plasmid...

  15. Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing

    Type
    Blog Post
    ... year, narrow by article type (review, patent, clinical study, research article etc). There are also numerous...conclusions. In any case, the reader should give a critical eye to all results in the relation to how they... biochemistry instructor in the Department of Biological Sciences at Louisiana State University. She is...

  16. Lab Automation at Addgene

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    Blog Post
    ...bacteria through the mail due to laws concerning genetically modified organisms. Since Addgene is very proud...individually. Enter the BIOROBOT! This robot is called the Qiagen BIOROBOT Universal Platform and it suits... for a mini prep, except it does it on a larger scale. Using the BIOROBOT Universal Platform is ideal ...

  17. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

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    Blog Post
    ...., 2021). Another version of the spike protein, called HexoPro, was developed by Jason McLellan’s lab....genome-wide CRISPR libraries, Brunello (knock out) and Calabrese (activation), from Addgene to better understand...bidirectional CRISPR screens identify mucins as critical host factors modulating SARS-CoV-2 infection. ...

  18. Meet an Addgenie: Insights into Grad School, Career Paths, and More

    Type
    Blog Post
    ...Addgene’s Lab Manager, was taking classes at her local community college when she took her first job at... and hoping to pursue. The choice to not go to medical school was devastating. At the same time, she has...circumstance. She was working as a research and technical assistant, when her life changed––she had a daughter...

  19. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

    Type
    Blog Post
    ... to be re-engineered for each gene, making wide-scale gene activation seem next to impossible. The development...methods greatly improved CRISPRa, expanding its practicality and popularity in research (Tanenbaum et al....JS, Nishimasu H, Nureki O, Zhang F (2014) Genome-scale transcriptional activation by an engineered CRISPR-Cas9...

  20. 6 Tips for Grant Writing

    Type
    Blog Post
    ...unintelligible to a lay audience. My lay paragraph typically includes a couple of sentences of introduction... rejected on some occasions. Strategize, choose calls that suit your career stage / research area, and...peers and collaborators can also help you get some critical insight into what you're doing wrong. All this...
Showing: 701 - 720 of 1019 results