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Showing: 701 - 720 of 985 results

  1. Changing Labor Laws Bring Increased Postdoc Wages

    Type
    Blog Post
    ...around $45,000, but as I’ve discussed elsewhere (slides here) there are many postdocs paid at much lower...expected for postdocs in many cases should be as described in the notice of the new NRSA levels and in the...

  4. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    ...updated Aug 20, 2020. Cas9 can be used to modify any desired genomic target provided that (1) the sequence is... may be unintentionally mutated along with the desired target locus. The PAM sequence is of particular...

  5. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    ...polymorphisms (SNPs) from human saliva. SHERLOCKv2 provides a method to detect nucleic acids with high sensitivity...per second. A quantifiable fluorescent signal designates the presence of your DNA of interest, in this...

  6. Plasmids 101: Plasmid Incompatibility

    Type
    Blog Post
    ... Environmental Plasmids blog). Scientists have designed small, high copy incompatible plasmids that lead...Pubmed PMID: 25889267 Ebersbach G, Sherratt DJ, Gerdes K (2005) Partition-associated incompatibility caused...

  8. Antibodies 101: The Four ELISAs and When to Use Them

    Type
    Blog Post
    ...staining. Antigen-containing samples often include undesirable molecules that may bind non-specifically to assay...specific than other methods. In addition, the assay includes both primary and secondary incubation steps, increasing...

  9. Plasmids 101: Stringent Regulation of Replication

    Type
    Blog Post
    ...replicate—but not always to the same extent. As described in our previous Origin of Replication post, DNA...replication. For example, the plasmid ColIb-P9 encodes the protein RepZ to drive its own replication. ...

  10. Viral Production at Addgene

    Type
    Blog Post
    ...protocols, titering methods, and quality control are described below. AAV Production AAV distributed by Addgene...) are generated from lentiviral preparations (described above) that are subject to a concentration step...

  11. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    ...best to start from scratch. Obtain a vial of the desired cell line from a reputable distributor such as ...extreme changes in osmotic pressure and pH can destabilize the viral envelope. One drawback of the multiple...

  13. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    ...flattening the curve, it has become evident that widespread testing is crucial in controlling this pandemic...one temperature. Two Cas12a-crRNA complexes are designed, each binding to one strand of the reverse-transcribed...

  14. Hot Plasmids: Summer 2024

    Type
    Blog Post
    ... A) Schematic of workflow. B) Detail of spacer peptides (3HB: 11-nm 3-helix bundle; SAH: 60-nm single ...cryo-EM samples from damage at the AWI. Plus, tardigrades!  Find RvLEAMshort here!    Abe, K. M., & Lim...

  15. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    ...antibody-based technique, validation and proper experimental design are essential. In this article, we’ll go through...steps include: counterstaining, mounting sample on slides, and confocal   Figure 2: High-level overview...

  16. Is this the right place for me? 8 tactics for choosing a lab

    Type
    Blog Post
    ... – including undermining personal integrity, destructive innuendo and sarcasm, persistent teasing, name...impossible deadlines, unnecessary disruptions Destabilization – including failure to acknowledge good work...

  17. Plasmids 101: Restriction Cloning

    Type
    Blog Post
    ...single enzyme if your insert is flanked on both sides by that enzyme’s restriction sites, but the insert...the orientation of your insert. You may want to design a diagnostic digest for this purpose. Ideally, ...

  19. Synthetic Biology - Algal

    Type
    Collection
    ...available from this depositor's lab. Plasmid Description Gene/Insert Vector Type PI Publication Do you...

  20. Synthetic Biology - Bacterial

    Type
    Collection
    ...available from this depositor's lab. Plasmid Description Gene/Insert Vector Type PI Publication Do you...
Showing: 701 - 720 of 985 results