We narrowed to 824 results for: EED

1. Design Tips for Prime Editing

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   Blog Post

   ...’re just starting out with prime editing, you’ll need to design and optimize a pegRNA for your particular...

2. Plasmids 101: The Wide World of Natural Plasmids

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   Blog Post

   ...direct transfer from one cell to another). Bacteria need special fertility genes to be capable of conjugation...

3. Viral Vectors 101: Calcium Sensors

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   Blog Post

   ... single GFP or CFP/YFP-derived FRET pair. If you need to free up crowded optical space for multiplexing...

4. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

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   Blog Post

   ...sample. The PCR is then performed, optimized as needed, and cleaned up using a standard PCR cleanup kit...

5. Adeno Associated Virus (AAV) for Cell and Gene Therapy

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   Blog Post

   ...deliver gene therapies directly to the cells that need them. Adeno-associated virus (AAV) – based delivery...

6. 5 Tips for Troubleshooting Viral Transductions

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   Blog Post

   ...volume. Always remove packaging cell debris before proceeding to ultracentrifugation to avoid contaminating...

7. Plasmids 101: Terminators and PolyA signals

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   Blog Post

   ...Plasmids 101 eBook to get all the background you need to start working with plasmids! What are termination...

8. Tips for Titering Your Lentiviral Preps

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   Blog Post

   ...fluorescent markers are used as it eliminates the need for antibody staining. Check Out Our In-Depth Fluoresence...

9. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

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   Blog Post

   ...datasets and download the custom python scripts needed to discover circRNAs in RNA-seq data. As far as...

10. How-to: 5 Steps to a Great Panel Discussion

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    Blog Post

    ...microphone system ready for each panelist if the room needs it (lapel mics are best). The moderator takes control...

11. The Scientific Conference Poster Session: Tips for Success

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    Blog Post

    ...Your colleagues, lab mates, and PI can provide feedback on your technical presentation, but those outside...

12. Tips to Make the Most of a Scientific Conference

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    Blog Post

    ...during the conference, you can try getting up to speed on social media. If you haven’t already, connect...

13. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

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    Blog Post

    ...subtilis (ICEBs1) system. ICEBs1 carries the genes needed for conjugation, but can also carry cargo genes...

14. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

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    Blog Post

    ...as homozygous alleles, others cannot. Geneticists need a method for maintaining stable lines of genetic...

15. Truncated gRNAs for Regulating Gene Expression

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    Blog Post

    ...already express nuclease-competent Cas9, obviating the need to generate new cell lines or transgenic animals...

16. Hot Plasmids and Viral Preps - May 2021

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    Blog Post

    ... easier for you to find and use the plasmids you need. Here's what you'll find in this post: Pooled library...

17. Hot Plasmids: Winter 2025

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    Blog Post

    ...the potential to improve rapid and precise plant breeding. Find dicot prime editing plasmids here! Vu, ...

18. Degrading DNA with Cascade-Cas3

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    Blog Post

    ...continued. However, Cas3 cannot bind DNA on its own and needs to remain anchored to Cascade throughout the process...

19. The AAVantages of AAV in CRISPR Screens

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    Blog Post

    ...Cas effectors in specific tissues, eliminating the need to deliver the Cas protein altogether. Platforming...

20. Twenty Years of Addgene Sharing: CRISPR

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    Blog Post

    ... Addgene depositors make CRISPR tools that fill needs in the community. The rapid adoption of new tools...