We narrowed to 807 results for: Eng;

1. Supporting Rare Disease Research with the 2020 BeHEARD Awards

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   Blog Post

   ... Empower and Accelerate Research Discoveries) Challenge provides grants for rare disease research. This...

2. RNA Extraction Without A Kit

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   Blog Post

   ...until homogenization and cell lysis. This can be challenging depending on your laboratory situation or tissue...

3. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

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   ...editors at Addgene. Levy et al., Nature Biomedical Engineering, 2020. PubMed PMID: 31937940. Detection of neural...

4. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

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   ...structural knowledge of the proteins. To overcome this challenge, the Uppalapati and Woolley lab developed a phage...

5. Reproducibility for Everyone: Lessons from an Open Science Collaboration

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   ...working with communities, not for them - Another strength of this collaboration is that it is driven by ...

6. Human Germline Editing Using CRISPR

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   Blog Post

   ...heterozygous mutations, it will clearly be much more challenging for homozygous mutations. Moreover, introducing...

7. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

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   Blog Post

   ...new plasmids and/or cells or organisms that are engineered can be shared to simplify the comparison by others...

8. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

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   ...~6.5 - 9 *Optimal sequential excitations wavelengths as determined by Rosado et al: see Table 2 for...

9. RaPID Detection of RNA-protein Interactions

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   Blog Post

   ...bind an RNA motif of interest in living cells. Challenges with existing RNA-protein interaction methods...

10. Summer SciComm: Preprints

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    Blog Post

    ...and feedback giving authors the opportunity to strengthen the manuscript prior to submission. Preprints...

11. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

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    Blog Post

    ...Galvan et al., 2019). The hM4Di receptor is an engineered Gi protein-coupled receptor, one of the Designer...

12. Viral Vectors 101: An Introduction to AAV

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    Blog Post

    ...and a self-complementary AAV (scAAV) vector. Full-length ssAAV vector genome of both polarities are rescued...

13. Transferable Skills Guide: Public Speaking

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    Blog Post

    ...gives your entire audience a chance to comfortably engage with you. If you can give a seminar, lead a journal...

14. The time and cost required to make a plasmid

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    Blog Post

    ...these rough estimates, it’s clear that community engagement with and use of repositories like Addgene provides...

15. Plasmids 101: The protein expression toolbox

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    ...promoters Tetracycline-dependent promoters are engineered by placing tet response elements upstream of ...

16. Developing Lab Management Software for Biology

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    Blog Post

    ...in on the fun too. Kris Shamloo is a Software Engineer at Addgene and is interested in writing software...

17. A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells

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    Blog Post

    ...T-to-G•C base changes (Figure 1), but it can be challenging to make these base changes, especially in primary...

18. A Guide to Designing a Scientific Poster: Content, Layout, and More

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    Blog Post

    ...outside of the reader’s field of view because of its length, and will be more difficult to read. The Thompson...

19. BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

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    Blog Post

    ...features that allow such “chemical rescue” in vivo. Engineering the human disease alleles into cells for testing...

20. Adenoviral Vector Production and Troubleshooting

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    Blog Post

    ...not integrate into the host’s genome. The main challenge with using AdV is that it triggers a strong immune...