Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 982 results for: IGH@

Showing: 701 - 720 of 982 results

  1. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    ...standard curve for qPCR can be challenging to get right. Here at Addgene, we use a linearized plasmid standard...very few positive droplets. In the image to the right, there is approximately 1 positive copy per microliter...droplet generator to the PCR plate. Maintaining a high droplet count is important for calculating the concentration...

  2. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

    Type
    Blog Post
    ... #MolluscMonday: #Crepidula atrasolea juvenile, light microscope with polarizing filter. Development is...an extreme number of nerve cells and an unusually high level of RNA recoding in the brain. RNA editing ...DNA in the eggs to get transgenic animals. They might be immortal. Manihot esculenta (Blake Meyers) Cassava...

  3. Designing Your Chalk Talk for the Academic Job Interview

    Type
    Blog Post
    ...million dollar sales pitch. Given this large sum, one might expect that applicants would work for months to ...research? This is the central idea. It should be at a high level, broadly understandable to scientists outside...engaged by your presentation, then you’re on the right track. Furthermore, practice getting back to your...

  4. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    ...data inside the ELN is reproducible, and only the highly restricted number of people who can access the ...practical to move all this data in bulk into a lightweight tool like an ELN. It is nevertheless critically...

  5. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    ...particular gene. Furthermore, a target sequence may have high homology elsewhere in the genome. These off-target...Sontheimer EJ (2018) NmeCas9 is an intrinsically high-fidelity genome-editing platform. Genome Biol 19...Evolved Cas9 variants with broad PAM compatibility and high DNA specificity. Nature 556:57–63 . https://doi....

  6. Viral Vectors 101: Preparing Pooled Libraries

    Type
    Blog Post
    ...depositing lab. (For our more popular libraries, you might receive a similarly-sized aliquot from an amplification...ll want to infect your cells with the virus–the right amount of virus, that is. Ideally, each cell will...Konermann S, Gootenberg JS, Abudayyeh OO, Platt RJ, Brigham MD, Sanjana NE, Zhang F. Genome-scale CRISPR-Cas9...

  7. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    ...interested audiences quickly and concisely – video might be part of the answer. Problems with written publications...TedTalks are very popular, though they focus mostly on highly innovative ideas rather than on the day-to-day ...that provides links to the videos, keeping the copyright and control over the videos with the researcher...

  8. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    ...recombination at a frequency three orders of magnitude higher than identical targeting vectors delivered through...Other investigators who had been working to develop high efficiency gene targeting approaches improved on...repair, it has proven more challenging to develop high efficiency CRISPR-based approaches for the generation...

  10. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    ...important that your gRNA sequence is designed to be highly active and reduce off targets. For CRISPRa and ...consuming process and should not be undertaken lightly! Indeed, truly achieving the gold standard requires...genetic screens using genome-wide libraries have shown high concordance between different sequences targeting...

  11. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    ... LITE system, which uses light to regulate transcription, also includes light-regulated histone methyltransferases... CA (2017) CRISPR–Cas9 epigenome editing enables high-throughput screening for functional regulatory elements...

  12. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    ...may be a good choice for targeting neurons, with high transduction frequencies in the central nervous ... our in-stock viral prep service.  AAV8 AAV8 has high transduction efficiency in multiple tissues, rapid...AAV9 AAV9 has relatively broad tissue tropism and high transduction efficiency, and is an excellent choice...

  13. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    ...using them. Indeed, the future of mouse work is bright as it is now easier than ever to manipulate the...technology, CRISPR, that is simple in concept, straightforward to use, and robust in performance. In its natural...BL, Xavier RJ, Root DE (2014) Rational design of highly active sgRNAs for CRISPR-Cas9–mediated gene inactivation...

  15. CRISPR Guide Update

    Type
    Blog Post
    ...existing content. Access the CRISPR Guide here! Highlights include a much-anticipated prime editing section...

  18. It’s CRISPR Week at Addgene!

    Type
    Blog Post
    ...generating knockouts using CRISPR and Wednesday will highlight some Cas9 variants. Next, we’ll look at various...

  19. The 12 Days of CRISPR

    Type
    Blog Post
    ...resources! Ready to sing along with this year's lineup? Right! We're ready to, so let's get started!  On the...sequences🎵On the 2nd day of CRISPR, Addgene gave to me: High-fidelity Cas13 variants for targeted RNA degradation...

  20. The Twelve Days of CRISPR

    Type
    Blog Post
    ...from the first generation of base editors to the eighth generation (and dual base editors, too!).  On the...gave to me: CRISPR methods for bacteria.  On the eighth day of CRISPR, Addgene gave to me: anti-CRISPR ...
Showing: 701 - 720 of 982 results