We narrowed to 1,093 results for: IND

...Addgene.org Find Fluorescent Protein Empy Backbones Visit Our Fluorescent Protein Guide Page Find Luciferase...more is often better. More transcription factor binding sites in a promoter lead to higher transcriptional...the power of SunTag for single molecule imaging, finding that plasma membrane-targeted CAAX-SunTag was 18...

...January. This involves going in front of a camera and kind of walking them through the steps of, for example... says Caitlin. Caitlin also works to meet the individual needs of schools interested in using eScience...what I did as an SME, and then 75% of the time I’m kind of the go-to person for my department.” Caitlin ...Addgene podcast with educator and science rap mastermind Tom McFadden Get tips about management for scientists...

...screening system and plans to employ the light‐inducible bacterial expression system pDawn to clone and...for their potential agricultural and medical use. Find More Optogenetic Tools Like pDawn  Making cassava...supplements Dr. Mrinalini Nair, University of Baroda, India Dr. Mrinalini Nair, Associate Professor (Right),...aims to leverage a glycine oxidase reaction to indirectly detect glyphosate via H2O2, one of the reaction...

...companies can recycle plastic-- you just have to find them (or keep reading). Corning takes back and recycles...take to action! These days, it’s important for individuals, companies, and universities to be sustainability... the Addgene blog Subscribe to the Addgene blog Find lab tips for your research Read another blog post...other scientists save resources by sharing plasmids Find plasmids at Addgene ...

... bench work. To really be happy, you may need to find a way to do, manage and lead all in one day.  How...is the ability to assess and initiate things independently. Taking initiative doesn’t mean going rogue,...listed a few of my favorites. Additionally, you can find more suggested resources on this topic and get the...Articles on Leadership, also can be purchased individually) ...

...Just as physical barcodes help librarians track individual books, genetic barcodes allow researchers to ... town! Pooled libraries can be designed for all kinds of different high-throughput experiments. Our most...species. This approach is useful for screening for binding properties. For example, several coronavirus libraries...teaching us more about viral evolution and antibody binding. Figure 3: Schematic of phage display screening...

...Resources on Addgene.org Find AAVs at Addgene Learn more about Addgene's viral service Find protocols for working...tagmentation-based library preparation procedures. This finding eliminates the double-stranding step and reduces... Lecomte E, Tournaire B, Cogné B, Dupont J-B, Lindenbaum P, Martin-Fontaine M, Broucque F, Robin C, Hebben...

...scientists using Addgene AAV viral preps to easily find and share useful technical data. These data focus...usage of these tools, which aren’t always easy to find in publications, so this is a much-needed community..., scientists can use the AAV Data Hub to quickly find details they need for planning their next experiments....org Read our AAV guide Browse all  AAV vectors Find ready-to-use AAV preps ...

... try to be as chronological as possible — if you find yourself jumping backwards in time to explain something...your lab or science or your CV has grown as well. Find your audience and fit them You don’t need to explain... paper. Build lots of versions of your story and find the one that fits your audience. Enjoy the story...Check out our blog posts on science communication Find interviews scientists in SciComm careers Check out...

...can always head over to the Viral Service page to find the full list of what’s available or suggest additional...beginners just starting work with virus. You can find all of our viral vector blog posts here. If you ...    www.addgene.org On the Addgene website you’ll find our updated viral vector pages as well as expanded...Addgene.org Overview of Viral Production at Addgene Find Virus Protocols Browse All Viral Vectors ...

...potentially rebind and nick the newly ligated DNA. Salt helps prevent topoisomerase I from rebinding, which...the "A" gets added to all PCR products. Keep in mind that Taq polymerase has an error rate of about 1...to Verify Your Plasmid Resources on Addgene.org Find More Plasmid Cloning Protocols Catch Up On Your ...

...Hybridization Sequence: The region of the primer that binds to the sequence to be amplified (usually 18-21bp... we want to amplify and design primers that will bind to and replicate it. The following image shows the...5'-ATGTGGCATATCTCGAAGTAC-3' for the region that binds the ORF and we will add the EcoRI restriction site... Reference Pages Deposit Your Completed Plasmids Find the Perfect Plasmid for Your Research ...

...and visualize. For more details on this procedure, find the protocol here.   Tips and troubleshooting ...Super-resolution mapping of neuronal circuitry with an index-optimized clearing agent." Cell reports14.11 (2016...Meng-Tsen, and Takeshi Imai. "Optical clearing and index matching of tissue samples for High-resolution fluorescence...viral service See our fluorescent protein collection Find AAVs at Addgene ...

... but did you know that Addgene also performs independent research? Since launching the viral service in...cells treated with sucrose or other osmolytes to induce hypertonicity display enhanced monoclonal antibody...blog Read our beginner's guide to viral vectors Find all of our viral vector blog posts Download our ...Addgene's viral service Learn about viral production Find viral protocols here ...

...they added flanking loxP sites and a heat-shock inducible Cre to generate a self-excising cassette (SEC)...genetics.115.178335. [Epub ahead of print] PubMed. Find plasmids from this publication at Addgene. Other...: 10.1038/nmeth.2641. Epub 2013 Sep 1. PubMed. Find plasmids from this publication at Addgene. Scalable...genetics.114.170423. Epub 2014 Sep 23. PubMed. Find plasmids from this publication at Addgene. Additional...

...having a mentor, developed some strategies for finding mentors and, perhaps, asked someone to support ...identified. Keep a journal to record topics, plans, findings, and progress over the course of an ongoing mentoring...Forced Choice Analysis is a fun example and you can find it in our Mentoring for Scientists Guide.  You can...steps (e.g. Meet 10 PhDs with interesting careers, find a summer internship, etc.). It is helpful to work...

...CD8 or CD4 epitopes, as well as their potential binding affinity to major histocompatibility complexes ...not yet possible for these algorithms to model individually-specific factors, such as previous exposures...prediction of immunogenicity is to validate the findings from the in silico assessments, as well as analyze...dentritic cells or macrophages through cytokine induction (Groell et.al, 2018). Thus, microscopic in-vitro...

... monogenetic diseases, the bulk of which you can find below. CRISPR for complex genetic diseases As CRISPR...single-guide RNA targeting the mutant allele (7). An independent team led by Hans Clevers at Hubrecht Institute...fibrosis report. “This is a big advantage over induced pluripotent stem cells, which need to go through...affects multiple organ systems. Nevertheless, the findings are proof-of-principle for use of CRISPR to correct...

... has a web and desktop version that works with Windows, Mac, and Linux operating systems.  Zotero  Zotero...desktop based program that’s compatible with Mac, Windows, and Linux.  Mendeley and Zotero have a lot of ...two citation managers. If you want more tips on finding and organizing papers, check out this related blog... blog post: Early Career Researcher Toolbox: Finding Relevant Papers. These are only a few of the many...

...motivated to do anything and everything we can to help find a cure -- and quickly. Normally drug discovery is...and help us figure out what is going on there!).  Find the Krogan Lab SARS-CoV-2 plasmids (Note: The Nsp6...over the world, as far flung as the EU, Russia, India and Saudi Arabia -- as well as closer to home in...QBI_UCSF and KroganLab), Instagram, and Facebook. Find more COVID-19 and coronavirus related plasmids and...