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We narrowed to 1,076 results for: IND;

Showing: 701 - 720 of 1076 results

  1. Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation

    Type
    Blog Post
    ...Resources on Addgene.org Find AAVs at Addgene Learn more about Addgene's viral service Find protocols for working...tagmentation-based library preparation procedures. This finding eliminates the double-stranding step and reduces... Lecomte E, Tournaire B, Cogné B, Dupont J-B, Lindenbaum P, Martin-Fontaine M, Broucque F, Robin C, Hebben...

  2. Celebrate Open Data Day with Addgene’s AAV Data Hub!

    Type
    Blog Post
    ...scientists using Addgene AAV viral preps to easily find and share useful technical data. These data focus...usage of these tools, which aren’t always easy to find in publications, so this is a much-needed community..., scientists can use the AAV Data Hub to quickly find details they need for planning their next experiments....org Read our AAV guide Browse all  AAV vectors Find ready-to-use AAV preps ...

  3. Tetbow: Bright Multicolor Labeling for Neuronal Tracing

    Type
    Blog Post
    ...and visualize. For more details on this procedure, find the protocol here.   Tips and troubleshooting ...Super-resolution mapping of neuronal circuitry with an index-optimized clearing agent." Cell reports14.11 (2016...Meng-Tsen, and Takeshi Imai. "Optical clearing and index matching of tissue samples for High-resolution fluorescence...viral service See our fluorescent protein collection Find AAVs at Addgene ...

  4. Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation

    Type
    Blog Post
    ... but did you know that Addgene also performs independent research? Since launching the viral service in...cells treated with sucrose or other osmolytes to induce hypertonicity display enhanced monoclonal antibody...blog Read our beginner's guide to viral vectors Find all of our viral vector blog posts Download our ...Addgene's viral service Learn about viral production Find viral protocols here ...

  5. Even more elegant: Single injection CRISPR/Cas9 in C. elegans

    Type
    Blog Post
    ...they added flanking loxP sites and a heat-shock inducible Cre to generate a self-excising cassette (SEC)...genetics.115.178335. [Epub ahead of print] PubMed. Find plasmids from this publication at Addgene. Other...: 10.1038/nmeth.2641. Epub 2013 Sep 1. PubMed. Find plasmids from this publication at Addgene. Scalable...genetics.114.170423. Epub 2014 Sep 23. PubMed. Find plasmids from this publication at Addgene. Additional...

  6. Mentoring for Scientists: I Have a Mentor, What Now?

    Type
    Blog Post
    ...having a mentor, developed some strategies for finding mentors and, perhaps, asked someone to support ...identified. Keep a journal to record topics, plans, findings, and progress over the course of an ongoing mentoring...Forced Choice Analysis is a fun example and you can find it in our Mentoring for Scientists Guide.  You can...steps (e.g. Meet 10 PhDs with interesting careers, find a summer internship, etc.). It is helpful to work...

  7. Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS

    Type
    Blog Post
    ...or off. Gal4 is a transcriptional activator that binds to UAS enhancer sequences found in DNA. It then ...recruits transcription machinery to the site to induce gene expression. Thus, genes and siRNA encoded ...neuronal compartment, you could express them individually or simultaneously. Do note for control purposes... much protein as overexpression of each gene individually. Applications of the Gal4/UAS system There are...

  8. Four Factors that Differentiate the Stem Cell Field

    Type
    Blog Post
    ... Resources on the Addgene Wesbite Find Reprogramming Plasmids Find Other Hot Plasmid Tools Visit Our ...paper showing that it was possible to generate induced pluripotent stem cells (iPSCs) capable of forming...like an understatement. Multiple speakers from top minds in the stem cell field have described the Yamanaka...

  9. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    ... and higher pH. We initially used this screen to find R body mutants that couldn’t extend at a normal ...the ciliate. It’s thought that they function as a kind of armor to protect their host from predation (Petroni....1021/acssynbio.5b00237. PubMed PMID: 26814170. Find Plasmids from This Publication at Addgene 4. Petroni...Resources on Addgene.org Read our Hot Plasmids Articles Find Plasmids for Bacterial Expression ...

  10. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

    Type
    Blog Post
    ...ssDNA molecules in vitro. Figure 1: Cas9 binding to the cleaved DNA duplex. Image from Richardson...diseases in which sequence replacement holds some kind of fitness advantage but error-prone repair of the...on Your HDR Basics Learn How to Design Your gRNA Find the Ideal CRISPR Software for Your Needs Resources... on Your CRISPR Background with Our CRISPR Guide Find CRISPR Plasmids for Your Research Check out Our ...

  11. Creating accessible biology activities in schools with BioBits

    Type
    Blog Post
    ... my head and left me thinking: I wish I had this kind of experience earlier! After all, students build...PMID: 30083609. PubMed Central PMCID: PMC6070313. Find the plasmids from this publication at Addgene! ... Additional Resources on the Addgene Blog Find planarian tools for teaching developmental biology Use... Check out our molecular biology reference pages Find plasmids from the BioBits BrightTM publication here...

  12. Plasmids 101: NGS Quality Control for Pooled Libraries

    Type
    Blog Post
    ...DNA is harvested, much like one would prep an individual plasmid. However, lots of library DNA is needed...obtain about 100 to 1,000 bacterial colonies per individual plasmid in the library. Bacterial growth must...representing perfect uniformity (see Figure 3A). Addgene finds that this representation is most easily understood...amplification looks like, but by looking at these kinds of graphs, making sure few/no plasmids have dropped...

  13. Scientists Map the SARS-CoV-2-Human Interaction Network

    Type
    Blog Post
    ...motivated to do anything and everything we can to help find a cure -- and quickly. Normally drug discovery is...and help us figure out what is going on there!).  Find the Krogan Lab SARS-CoV-2 plasmids (Note: The Nsp6...over the world, as far flung as the EU, Russia, India and Saudi Arabia -- as well as closer to home in...QBI_UCSF and KroganLab), Instagram, and Facebook. Find more COVID-19 and coronavirus related plasmids and...

  14. FlipGFP, a novel fluorescence protease reporter to study apoptosis

    Type
    Blog Post
    ... Normally, β10-11 are antiparallel. To prevent binding, the β strands in FlipGFP were redesigned so that...cells expressing FlipGFP with staurosporine, which induces apoptosis by activating caspase-3, and detected...understand these diseases and disorders. You can find the plasmids for FlipGFP and FlipCherry at Addgene...Addgene.org Browse the fluorescent protein collection Find empty backbones for fluorescent protein fusions ...

  15. Improving Transduction Efficiency with Exosome AAVs

    Type
    Blog Post
    ...Learn about Addgene's viral service Find viral protocols and videos Find AAVs for your research ...scientists have yet to decipher the mechanistic aspects behind these improvements. What have exosome AAVs achieved...Addgene blog Read all of our viral vectors blog posts Find tips for preparing AAVs Download our viral vectors...

  16. Using CRISPR/Cas9 to Edit Disease Out of the Genome

    Type
    Blog Post
    ... monogenetic diseases, the bulk of which you can find below. CRISPR for complex genetic diseases As CRISPR...single-guide RNA targeting the mutant allele (7). An independent team led by Hans Clevers at Hubrecht Institute...fibrosis report. “This is a big advantage over induced pluripotent stem cells, which need to go through...affects multiple organ systems. Nevertheless, the findings are proof-of-principle for use of CRISPR to correct...

  17. Early Career Researcher Toolbox: Free Tools for Science Writing

    Type
    Blog Post
    ... has a web and desktop version that works with Windows, Mac, and Linux operating systems.  Zotero  Zotero...desktop based program that’s compatible with Mac, Windows, and Linux.  Mendeley and Zotero have a lot of ...two citation managers. If you want more tips on finding and organizing papers, check out this related blog... blog post: Early Career Researcher Toolbox: Finding Relevant Papers. These are only a few of the many...

  18. Predicting Adverse Reactions to Monoclonal Antibody Drugs

    Type
    Blog Post
    ...CD8 or CD4 epitopes, as well as their potential binding affinity to major histocompatibility complexes ...not yet possible for these algorithms to model individually-specific factors, such as previous exposures...prediction of immunogenicity is to validate the findings from the in silico assessments, as well as analyze...dentritic cells or macrophages through cytokine induction (Groell et.al, 2018). Thus, microscopic in-vitro...

  19. New and Upcoming Viral Vectors - September 2019

    Type
    Blog Post
    ...Learn about Addgene's viral service Find viral protocols and videos Find AAVs for your research ...serotypes, since these 20 ul samples are distributed individually. See our Serotype Testing AAV inventory for ...Addgene blog Read all of our viral vectors blog posts Find tips for preparing AAVs Download our viral vectors...

  20. Addgene Welcomes John Doench to the Advisory Board

    Type
    Blog Post
    ... Podcast.       Resources at Addgene Find CRISPR resources Find CRISPR pooled libraries Learn about other...editing and screening) such that thousands of individual edits can be studied quickly. Involvement with.... the nonprofit, sharing ethic that Addgene has behind it is something that very much resonates with me...
Showing: 701 - 720 of 1076 results