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...website, as an instructive step-by-step guide.  Finally, when writing your alt text, use all of this information... equal to the buoyant force of the solution. Contaminants of lower densities are captured at the 17%, ...

...fluorophore, which emits light. No excitation illumination needed — just the luminescent substrate. This...you used? Share your tips below! This post was originally written by Jason Niehaus in June 2014 and was...

...supportive environment to build my management skills. Finally, developing emotional intelligence is vital. Learning...collaboration. The good news: It’s a journey, not a final choice Management is not a one-way path. As careers...

...biology – they can deliver materials to specific destinations, haul a variety of cargo, and even handle a ...applications Vaccine development Attenuated and inactivated viruses pioneered vaccine development well over...

...them ideal for applications with whole organisms. Finally, viral delivery is typically straightforward across...for multiple diseases, including hemophilia B and spinal muscular atrophy.   Type of Virus Pros Cons ...

...opportunity for homologous recombination. Before CRISPR, that recombination was a one in a million event...

...Ku then acts as a scaffold for recruitment of a kinase (DNA-PKcs) and a two subunit DNA ligase (XRCC4-...reduces breaks and mutations at off-target sites. Christina Mork contributed to the update of this post. Thank...

...interested in the same phosphate of your favorite kinase is a good start. Check out Joanne's Reddit AMA ...Networking - Building Relationships for Success" webinar with Joanne Kamens via ASCB Check out more Career...

...plasmids out of the library in the appropriate combination and do a restriction enzyme digest to cut out...scientists relied on traditional homologous recombination. That technology had been used for decades to...

... (such as LTRs or ITRs), hairpins, or strong terminators. Detailed instructions on other factors to consider...when designing your primers can be found here. Finally, you will want to have a well-organized database...

...LinkedIn may revoke your ability to send invitations. Finally, if you don’t know clearly why you are inviting...updates, or click bait of any kind. For more on inappropriate content see this article. However, do comment...

... DNA that you want to assemble together in a combination of different ways according to your cloning strategy... or PCR products from a gel. You will avoid contamination from other DNA fragments and you will remove...

... during, and after organizing such a workshop. Finally, the group put a lot of effort into ensuring that...them can do more harm than good. Check out this webinar for more on this. Be on the lookout for upcoming...

...expressed. Pho8Δ60 localizes to the cytosol in an inactive form unless autophagy is induced. Then non-selective... Pho8 from its uncleaved to cleaved form is the final read out of this assay. This is also a quantitative...

...plasmid copy number in a cell. One can attempt to eliminate fluctuations due to copy number by introducing...multi-protein complex, stabilized promoters can eliminate variability between the different components. ...

...easy detection with the appropriate fluorescent illumination in in vivo, ex vivo, or post-mortem experiments...Yerkes National Primate Research Center. Using a combination of in vivo and anatomical techniques, her research...

...combine the best of these mutations and tested the combination mutants for their on-target and off-target activity...that eSpCas9(1.1) and SpCas9-HF1 each stall in an inactive form after binding to a target site and propose...

...was contributed by Johnna Roose. This post was originally published on Johnna's New Under The Sun Blog ...In others, the experiments are geared towards eliminating possibilities until the results focus in on a...

...complexity. We have characterized almost 600 terminators. That gives us enough hardware to control hundreds...over the next year. Then there will both be a combination of parts in Addgene along with the software to...

...carrying through on the actions you committed to. Finally, hiring a paid coach will make you take the experience...sensitive to this and have coaching plans that are financially accessible. Sometimes only a few hours of coaching...