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  1. Hot Plasmids - January 2023

    Type
    Blog Post
    ...JA. Precise transcript targeting by CRISPR-Csm complexes. Nat Biotechnol. 2023 Jan 23. doi: 10.1038/s41587...antibodies allows you to use them in parallel to help amplify your signal - giving you the benefit of a polyclonal...

  3. Choosing Your Fluorescent Proteins for Multi-Color Imaging

    Type
    Blog Post
    ... proteins should be separated by 50-60 nm. For example, CFP (ex 430 nm / em 474 nm) and YFP (ex 514 nm...protein of interest has a similar spectrum. For example, here's a screenshot from the Chroma Spectra Viewer...

  4. Fluorescent Proteins 101: Luciferase

    Type
    Blog Post
    ...and improved signal sensitivity since photons are simply measured – they are not required to initiate the... will result in luciferase expression and more complex cell treatments can be designed to further explore...

  5. Communicating Your Science With Help From ComSciCon

    Type
    Blog Post
    ...an interview setting, this means persuading an employer that your past work has prepared you to contribute...audience and ask for their feedback before you complete your work. If you’re giving a public talk, ask...

  6. Why and how to start studying chromatin (with Addgene's help)

    Type
    Blog Post
    ...interact with each other. Chromatin regulation is complex and influences--directly or indirectly--many other...phosphoinositol-dependent binding of the SWI/SNF-like BAF complex to chromatin after T lymphocyte receptor signaling...

  7. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    ... handy. This approach is especially suited to complement optogenetic tools, where light used for excitation...external light source. Therefore, especially thick samples may not be appropriate for use with Nano-lantern...

  9. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    ...conditions in order to maximize the mutation rate. For example the Phage Assisted Continuous Evolution (PACE) ...interest. A gRNA is used to direct the PolI3M-nCas9 complex to a DNA site of interest, which nCas9 nicks and...

  10. Viral Vectors 101: Types of viruses

    Type
    Blog Post
    ...that use reverse transcriptase to synthesize complementary DNA copies of their genome upon infecting a ... These viruses have a cargo capacity of ~ 8 kb, ample room for most experimental needs, and can transduce...

  11. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    ...shRNAs are short RNA sequences which target complementary mRNA sequence for degradation. Viral introduction...targeting an exon in your gene of interest, for example. Some customization is required, but many viral...

  12. How Addgene Creates Content

    Type
    Blog Post
    ... look at how we create this content, using the example of our Antibody Guide, which was published alongside...require updating or retiring. CRISPR/Cas9, for example, has gone through rapid change in the past few ...

  13. Antibodies 101: Isotypes

    Type
    Blog Post
    ...portion of different antibody isotypes. Binding to complement system proteins, which can initiate a cascade... next to a different constant domain gene. For example if a B cell switches to producing IgE antibodies...

  14. CasPEDIA: A Functional Classification of Cas Enzymes

    Type
    Blog Post
    ... the cis and trans activity of each entry. For example, SpyCas9a (one of the most frequently used Cas ... an advanced search option. For the former, you simply type in identifier keywords (Cas name, RefSeq ID...

  15. A Deep Dive into BioBuilder

    Type
    Blog Post
    ... in March to exhibit projects at any stage of completion, from pitch to prototype. Last year’s BioBuilderClub... for a local school’s Science Department, or to simply tour the lab as a starting point into further STEM...

  20. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    ...modified GFP-dCas9 and the gRNA scaffold to enhance complex assembly, thereby decreasing the amount of background...additional hairpin/fluorescent protein combination, for example, with a far-red FP, would increase the available...
Showing: 701 - 720 of 929 results